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  • 1
    Electronic Resource
    Electronic Resource
    Storage of noncryopreserved periphered blood stem cells for transplantation (1996)
    Springer
    Annals of hematology 72 (1996), S. 303-306 
    Details
    ISSN: 1432-0584
    Keywords: Key words Peripheral blood stem cell ; CFU-GM ; Progenitor cell cloning assay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Mobilized peripheral blood stem cells (PBSC) were collected in autologous plasma and acid-citrate-dextrose formula A (ACD-A) by leukaphereses using the CS3000 cell separator (Baxter) and stored at 4  °C in a refrigerator for 8 days. We have looked at the viability of the nucleated cells with the trypan blue test and the proliferation and differentiation capacity using a standardized progenitor cell cloning assay. The changes in viability, granulocyte-macrophage colony-forming units (CFU-GM), erythroid burst-forming units (BFU-E), and mixed-lineage colony-forming units (CFU-GEMM) were determined daily during the storage period. Viability was 90.8% (SD 8%) at day 0 and declined to a mean of 69.5% (SD 15.5%) at day 8. CFU-GM decreased to 47% (SD 28.7%), CFU-GEMM to 48% (SD 42.2%), and BFU-E to 40.1% (SD 18.4%) after 6 days. After 5 days of storage the mean viability was 79.7% (SD 17.8%), whereas the mean CFU-GM were 65.3% (SD 28.4%) the mean CFU-GEMM were 61.8% (SD 30.4%) and the mean BFU-E were 55.1% (SD 18.2%). At day 4 viability was still 82.5% (SD 17.0%), recovery of CFU-GM was 78.5% (SD 28.8%), recovery of CFU-GEMM was 70.7% (SD 40.4%) and recovery of BFU-E was 65.0% (SD 17.5%). These data show, that PBSC can be stored safely over at least 5 days at 4  °C while the patient receives high-dose chemotherapy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002770050176
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Soluble CD56 (NCAM): a new differential-diagnostic and prognostic marker in multiple myeloma (1996)
    Springer
    Annals of hematology 73 (1996), S. 121-126 
    Details
    ISSN: 1432-0584
    Keywords: Key words Multiple myeloma ; Prognostic marker ; Neural cell adhesion molecule ; β2-Microglobulin ; Interleukin-6
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  A retrospective study was carried out to determine the diagnostic and prognostic value of the soluble form of the embryonal neural cell adhesion molecule NCAM (CD56) in paraproteinemia. NCAM, β2-microglobulin, and interleukin-6 levels were measured in the sera of 170 patients with paraproteinemia. Of these, 125 had multiple myeloma, 20 Waldenström's disease, and 25 monoclonal gammopathy of unknown significance. Serum NCAM proved superior to β2-microglobulin and interleukin-6 in distinguishing multiple myeloma from paraproteinemias of various causes, with a high specificity of 95.5% in detecting multiple myeloma, although with a low sensitivity of 40%. In multiple myeloma NCAM is significantly correlated with β2-microglobulin, paraproteinemia, and low albumin levels. For the determination of tumor load β2-microglobulin levels accord best with the Salmon and Durie classification scheme. When patients are grouped according to their clinical course, the disease development is reflected better by NCAM than by β2-microglobulin or interleukin-6. Survival data indicate that all three markers have prognostic potential. Prognostic accuracy with respect to overall survival is best with β2-microglobulin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002770050212
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  • 3
    Electronic Resource
    Electronic Resource
    Expression of vitamin D receptor in lung cancer (1996)
    Springer
    Journal of cancer research and clinical oncology 122 (1996), S. 356-359 
    Details
    ISSN: 1432-1335
    Keywords: Lung cancer ; Cell lines ; Vitamin D receptor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The active metabolite of vitamin D 1,25-dihydroxycholecalciferol is a hormone-like agent that regulates cell differentiation and proliferation. Various vitamin D derivatives have been shown to induce differentiation in neoplastic cells. The prerequisite for any hormone action is the presence of its receptor. We studied the expression of vitamin D receptor in human lung cancer cell lines and in primary lung cancer tissue. Employing the polymerase chain reaction, 10 out of 11 cell lines stemming from small-cell lung cancer and 15 out of 15 cell lines stemming from non-small-cell lung cancer demonstrated vitamin D receptor expression. An immunohistochemical analysis, using a specific monoclonal antibody, demonstrated vitamin D receptor protein expression in 31 out of 117 (26%) primary small-cell lung cancer cases tested. Positive cells exhibited a nuclear reaction pattern. Twenty-one out of 37 primary non-small-cell lung cancer cases, particularly adenocarcinomas (9/14) and squamous-cell carcinomas (10/15), exhibited vitamin D receptor. Results indicate that a subset of lung cancer cases may be susceptible to the differentiating effects of vitamin D analogues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01220803
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Immunologische Untersuchungen bei Wegenerscher Granulomatose mit Nachweis von γ-Globulin-Antikörpern (1964)
    Springer
    Journal of molecular medicine 42 (1964), S. 866-872 
    Details
    ISSN: 1432-1440
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary A case of Wegener's granulomatosis was characterised by a dysproteinaemia and a marked heterogenity of theγ-globulins in the serum and urine. By means of serological investigations a rheumatoid factor and another antibody toγ-globulin has been demonstrated in the serum of the patient. It is assumed that this is an antibody to autologousγ-globulin, which becomes autoantigenic in the pathogenesis of this disease. Relationships to periarteriitis nodosa and to rheumatoid arthritis have been discussed.
    Notes: Zusammenfassung Bei einem Fall von Wegenerscher Granulomatose fand sich neben einer Verschiebung des Proteinspektrums eine deutliche Heterogenität derγ-Globuline in Serum und Harn. Durch serologische Untersuchungen konnte ein Rheumafaktor sowie ein weiterer gegenγ-Globulin gerichteter Antikörper im Serum des Patienten nachgewiesen werden. Vermutlich handelt es sich hierbei um einen Antikörper gegen autologesγ-Globulin, welches bei dem Krankheitsprozeß zum Autoantigen wird. Verbindungen zur Periarteriitis nodosa und zur chronischen Polyarthritis werden aufgezeigt und diskutiert.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01488673
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Fifth I.A.S.L.C. Lung Tumor Biology Workshop, August 13–17, 1996, Ermatingen, Switzerland (1997)
    Springer
    Annals of oncology 8 (1997), S. 27-36 
    Details
    ISSN: 1569-8041
    Keywords: biology ; lung cancer ; molecular biology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008282400210
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    Paper (German National Licenses)
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