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  • 1
    Digitale Medien
    Digitale Medien
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 71 (1992), S. 5500-5503 
    ISSN: 1089-7550
    Quelle: AIP Digital Archive
    Thema: Physik
    Notizen: The influence of the crystallographic phase transformation on the work function was studied on a Co(0001) surface by a precise measurement of the contact potential difference. The characteristic hysteresis of the work function due to the hcp(arrow-right-and-left)fcc allotropic transformation was found. The work function changes abruptly by about 2 meV with the phase transformation while decreasing monotonically with temperature except in the transformation range. The contributions of the surface dipole barrier and the chemical potential of electrons to the work function are discussed from the point of view of cellular approximation.
    Materialart: Digitale Medien
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  • 2
    Digitale Medien
    Digitale Medien
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 73 (1993), S. 1100-1103 
    ISSN: 1089-7550
    Quelle: AIP Digital Archive
    Thema: Physik
    Notizen: The alloying behavior of copper atoms into gold clusters at temperatures lower than 300 K was examined using a 200 kV transmission electron microscope equipped with a double source evaporator in the specimen chamber. Isolated gold clusters of about 4 nm in mean diameter were prepared on a supporting film and were cooled down to 245, 215, 165, or 125 K. Copper atoms were then deposited onto the same film. Upon the deposition of copper, the gold clusters quickly changed into completely or partially alloyed clusters, depending upon the substrate temperature. The copper diffusivity estimated from such spontaneous alloying was many orders of magnitude faster than that in bulk gold. A possible mechanism behind the spontaneous alloying is discussed.
    Materialart: Digitale Medien
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  • 3
    Digitale Medien
    Digitale Medien
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 61 (1992), S. 2173-2174 
    ISSN: 1077-3118
    Quelle: AIP Digital Archive
    Thema: Physik
    Notizen: Alloying behavior of gold atoms into copper clusters has been studied using a 200 kV transmission electron microscope (TEM) equipped with a double source evaporator in the specimen chamber. Isolated copper clusters of approximately 4 nm in diameter were first prepared on a supporting film of amorphous carbon, and then gold atoms were deposited onto the same film kept at room temperature. Upon the deposition of gold, the copper clusters quickly changed into homogeneously mixed Cu-Au alloy clusters. The gold diffusivities in the clusters estimated from the experimental results are at least 19 orders of magnitude faster than those in the bulk copper.
    Materialart: Digitale Medien
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  • 4
    ISSN: 1365-3083
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: MyD88 is a key adaptor molecule for signalling via Toll-like receptors (TLRs) and the response to gut commensal microbes. To investigate the role of TLRs/MyD88 pathway in the development of the gut-associated lymphoid tissue (GALT), we examined the development of Peyer's patches (PPs) and cryptopatch (CP), and also one of effector compartment, intraepithelial lymphocyte (IEL) in MyD88–/–, TLR2–/– and TLR4–/– mice. In MyD88–/– mice, the organogenesis of PPs was not disturbed. However, PPs in 2-week-old MyD88–/– mice were significantly smaller than those in MyD88+/– mice. Also, in 2-week-old TLR4–/–, but not TLR2–/– mice, PPs did not develop rapidly. The development of PPs in MyD88–/– and TLR4–/– mice was completely recovered in 10 weeks. PP cells from MyD88–/– mice showed significant decrease in proliferation when stimulated with lipopolysaccharide. The development of CP and IEL was also normal in 10-week-old MyD88–/– mice. These results suggest that the TLRs/MyD88 pathway might be involved in the development of PPs only at early postnatal stage, and TLRs/MyD88-independent signalling is critically involved in the development of GALT in adult mice.
    Materialart: Digitale Medien
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  • 5
    ISSN: 1365-3040
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: The addition of gibberellin A3 (GA3) to culture media induced a dramatic elongation of the first internode in Hong Mang Mai, a wheat variety tolerant to deep-seeding conditions. The length of the first internode in the GA3-treated seedlings reached approximately 45 cm, which is twice as long as that of the control, whereas first internodes of other varieties were 3 to 12 cm long and hardly affected by GA3. The level of endogenous gibberellins (GA) in Hong Mang Mai was not greater than that of other wheat varieties. The expression of GAMyb, a transcription factor, was abundant in the first internode and substantially increased by GA3 application in Hong Mang Mai, compared with other wheat varieties. These results suggest that the first internode of Hong Mang Mai is more sensitive to GA in inducing strong elongation. The presence of potassium in the culture media was indispensable for the first internode elongation. Application of GA3 enhanced the uptake of potassium in Hong Mang Mai. Higher sensitivity of the first internode to GA could cause strong elongation by increasing the amount of osmotic solute, which plays an important role in the tolerance mechanism of Hong Mang Mai to deep-seeding conditions.
    Materialart: Digitale Medien
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  • 6
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 119 (2000), S. 0 
    ISSN: 1439-0523
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: The molecular and biochemical aspects of Tibetan β-amylase-less mutant barley were investigated. An enzyme assay showed that there were eight β-amylase-less mutant lines in the Tibetan landrace barley. Following Southern analysis and polymerase chain reaction, it is suggested that these mutants result from the same insert mutation in the latter part of the β-amylase structural gene. The α-glucosidase activity level of the mutant line was high in the initial period of germination, and the glucose ratio in the sugar conformation was remarkably high in the germinated seed of the mutant line. These results suggest that this β-amylase-less mutant line could be a new breeding material for beer production.
    Materialart: Digitale Medien
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  • 7
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 122 (2003), S. 0 
    ISSN: 1439-0523
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: The barley cultivar ‘Lenins’ was found to be a genotype showing high shoot regeneration ability in cultures derived from immature embryos. Five cultivars different from ‘Lenins’ in shoot regeneration ability were reciprocally crossed with ‘Lenins’ and the inheritance of tissue culture traits was investigated. F2 plants showed continuous distributions in callus growth and percentage of shoot regeneration, suggesting that these traits were controlled by polygenes. The F2 population, derived from a cross between ‘Lenins’ and ‘6721′, showed a monogenic segregation for the number of regenerated shoots, and the segregation ratio fitted 1:2:1. Tissue culture traits of ‘Lenins’ were controlled by several genes, whereas the number of regenerated shoots related to the efficiency of shoot regeneration is controlled by one major gene.
    Materialart: Digitale Medien
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  • 8
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 123 (2004), S. 0 
    ISSN: 1439-0523
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: A high-throughput single nucleotide polymorphism (SNP) genotyping procedure was developed to select amylose-free barley mutants whose waxy genes had a C- to T-base substitution in exon 5, which converted Gln-89 of the wild-type gene into a termination codon. An F2 population carrying an amylose-free waxy gene was checked for segregation. Polymerase chain reaction with confronting two-pair primers (PCR-CTPP) produced allele-specific PCR products that have different sizes and are inherited in a co-dominant manner. Two alleles of the barley waxy gene with SNP were correctly identified in parental strains using the PCR-CTPP procedure. Segregation of the SNP as detected by PCR-CTPP in an F2 population fitted the expected 1:2:1 ratio. The PCR-CTPP procedure can provide a time saving and cost-effective alternative to derived cleaved amplified polymorphic sequence in marker-assisted selection.
    Materialart: Digitale Medien
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  • 9
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 122 (2003), S. 0 
    ISSN: 1439-0523
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Eighty-four cultivars of barley (Hordeum vulgare) and 95 wild strains (82 of H. spontaneum and 13 of H. agriocrithon) were surveyed for the production of callus, callus growth, and shoot regeneration in cultures derived from immature embryos. All cultivars except for ‘Turkey 381′, induced calli from more than 90% of embryos. On the other hand, the wild lines showed a large variation in the percentage of callus induction from 0 to 100%. Among the cultivars, those with the brittle rachis genotype, bt Bt2, on chromosome 3H, regenerated shoots with a significantly higher percentage than the cultivars with the Bt bt2 genotype. Green shoots were produced in a higher ratio (0.84) in the cultivars than in the wild lines (0.52). Among the lines examined,‘Lenins’ regenerated shoots efficiently (90.4%) and produced the highest number of calli with green shoots per embryo (4.77) followed by ‘Golden Promise’ (3.15). Examination of callus growth and shoot regeneration from embryos at different developmental stages revealed that scutellum development affected the quantity and quality of callus and shoot regeneration.
    Materialart: Digitale Medien
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  • 10
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 23 (1993), S. 0 
    ISSN: 1365-2222
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: The relation between non-specific bronchial responsiveness and allergic sensitivity was evaluated in children with asthma. Bronchial responsiveness was determined by methacholine inhalation challenge test, and was expressed as a provocative concentration of methacholine causing a 20% fall in FEV1 (PC20). RAST titre for Dermatophagoides farinae and serum IgE level were evaluated as parameters of allergic sensitivity. When the PC20 vaiues, RAST titres for D. farinae. and serum IgE levels of 47 asthmatic children and 16 normal controls were compared. the asthmatic children had significantly lower PC20 values and higher D. farinae-RAST titres and serum IgE levels than the normal controls. The correlation analyses in 47 asthmatic children have shown that there is no significant correlation between PC20 values and RAST titres for D. farinae (r= 0.04. P 〉 0.1) or between PC20 values and serum IgE levels (r= 〈 0.03 P 〉 0.1). These results suggest thaty although both bronchial hyperresponsiveness and allergic sensitizalion may be in some way related to one another in children with asthma, the magnitude of allergie sensilization does not influence the degree of the non-specific bronchial responsiveness.
    Materialart: Digitale Medien
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