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  • 1
    Electronic Resource
    Electronic Resource
    Postnatal development of neuropeptide Y- and calcitonin gene-related peptide-immunoreactive nerves in the rat urinary bladder (1994)
    Springer
    Anatomy and embryology 189 (1994), S. 361-373 
    Details
    ISSN: 1432-0568
    Keywords: Postnatal development ; Neuropeptide Y ; Calcitonin gene-related peptide ; Urinary bladder ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The postnatal development of neuropeptide Y- and calcitonin gene-related peptide-immunoreactive (NPY-IR and CGRP-IR) nerve fibers in the rat urinary bladder was investigated using whole-mount preparations and cryostat sections. In newborn and 3-day-old rats, many NPY-IR nerve fibers were observed in the subserous and muscle layers. Many NPY-IR nerve cell bodies clustered at branching points of the subserous nerve bundles. Within 4 weeks after birth, these cell bodies drastically decreased in number and spread along the bundles, although the number of NPY-IR nerve fibers increased moderately. In contrast, CGRP-IR nerve fibers in newborn and 3-day-old rats were less developed, and no CGRP-IR nerve cell body was observed in any rat. However, CGRP-IR nerve fiber distribution in the urinary tissues conspicuously increased within 4 weeks after birth. Especially, an increase of the infraepithelial fibers showing a meshwork appearance was prominent in the fundus and corpus of the bladder. The infra- and intraepithelial CGRP-IR nerve meshwork of the ventral wall was more dense than that of the trigone. At 4 weeks, NPY-IR and CGRP-IR nerves were similar to those of the adult rat (8–12 weeks old). The present study suggests a correlation between the development of the peripheral nervous system in the urinary bladder and maturation of micturition behavior in the rat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00190591
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  • 2
    Electronic Resource
    Electronic Resource
    Nutrient Composition of Lamb of Two Age Groups (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 49 (1984), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The nutrient composition of Spring and Fall lambs were investigated. Seven retail cuts from carcasses of lambs raised under commercial conditions, and representing two age groups (4–4½ mo and 8–9 mo) were analyzed in both raw and cooked form. Separable lean meat was analyzed for proximate composition, 8 vitamins, 8 inorganic nutrients, cholesterol and 12 fatty acids. Except for moisture, total lipid, riboflavin, niacin, Zn and Fe, there were no practical differences in nutrients between cuts or age groups. Thiamin had the lowest cooking retention with a range of 29.0-63.5%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1984.tb14961.x
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  • 3
    Electronic Resource
    Electronic Resource
    Nutrient Composition of Fresh Retail Pork (1983)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 48 (1983), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The nutrient composition of fresh pork was studied in samples from 71 carcasses. On separable lean, nutrient composition was determined for 7 raw retail cuts from one side of each of 11 carcasses, and nutrient retention was determined on the 7 matching cuts from the other side that had been cooked by common household methods. Loins from 60 additional carcasses were analyzed to determine whether USDA grades 1, 2, and 3 and region of production affected nutrient composition. The data indicated that variation in nutrient composition of pork is more dependent on the retail cut within the carcass than either the grade or the region of production of the carcass. Cooking method significantly affected retention of most of the nutrients analyzed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1983.tb05080.x
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  • 4
    Electronic Resource
    Electronic Resource
    EFFECT OF ELECTRICAL STIMULATION ON CARCASSES FROM STRESSED AND UNSTRESSED STEERS (1983)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food quality 6 (1983), S. 0 
    Details
    ISSN: 1745-4557
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Twelve steers of known breeding and management history were assigned to two antemortem treatment groups (stress or no stress). After evisceration and splitting, sides in each group were randomly assigned to two electrical stimulation treatments (no stimulation or stimulated intermittently with one amp current for 2 min). ES did not affect any of the carcass characteristics in the stressed group except lean and overall maturity. Although ES did not affect lean color in stressed carcasses, they were darker in color than unstressed carcasses. The unstressed stimulated carcasses were more tender and contained less panel detectable connective tissue than the unstressed-unstimulated carcasses. ES did not improve tenderness of stressed carcasses. Thus, it appears that ES will be effective only on carcasses that have not severely depleted their stores of muscle glycogen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1745-4557.1983.tb00758.x
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  • 5
    Electronic Resource
    Electronic Resource
    Vitamin B12, E and D Content of Raw and Cooked Beef (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 47 (1982), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The relationship of carcass grade, primal cuts and cooking to vitamin B12, E and D in separable lean beef was studied. The average vitamin B12 content in 471 samples of raw and cooked beef was 3.17 μg/100g. The vitamin B12 content of raw and cooked beef was similar; however, on considering the moisture and fat losses during cooking, there was a 27–33% loss of B12. The vitamin E content of raw and cooked beef was similar and averaged 133 μg/100g for 464 samples. From 33–44% of the original vitamin E in the meat was lost upon cooking. Raw and cooked beef contained 80–100 ng of vitamin D/100g, with 35–42% of the original vitamin D content being lost upon cooking. The content of vitamins E and D in beef is low and of little nutritional importance; however, beef is an important dietary source of vitamin B12.〈section xml:id="abs1-2"〉〈title type="main"〉SUMMARY & CONCLUSIONSRAW OR COOKED separable lean contained approximately 80–100 ng of vitamin D/100g, with 35–42% of the original vitamin D being lost upon cooking. Raw and cooked separable lean contained approximately 40–800 μg of vitamin E/100g with a mean value of 133 μg for 464 samples. From 33–44% of the original vitamin E was lost during cooking. Fat trim contained 300–350 μg of vitamin E/100g. HPLC showed that α-tocopherol was the major E vitamer in meat. Vitamin B-12 content of raw and cooked separable lean ranged from 1 – 10 μg/100g, with a mean value of 3.17 μg B-12/100g for 471 samples. Fat trim contained approximately 1.5 μg of B-12/100g. RID was a feasible method for determining B-12 in meat, with the B-12 values reported herein being appreciably greater than typical values reported for B-12 in beef as determined by microbiological assays. Beef consumption makes an important contribution towards meeting an individual's dietary requirement for B-12.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1982.tb12883.x
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  • 6
    Electronic Resource
    Electronic Resource
    Sampling of Fresh Pork for Nutrient Analysis (1981)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 46 (1981), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Paired loins from 18 barrow and gilt carcasses were randomly selected to evaluate the effect of marketing on the nutrient composition of fresh pork loins. The paired loin from one side was shipped through a predetermined marketing system, while the control loin was transported immediately back to Beltsville, MD. Rib chops from each loin were analyzed for key nutrients. The overall results from this study indicate that pork loins subjected to extreme marketing conditions have weights, appearance and quality traits and nutritional composition similar to loins shipped from a slaughter plant directly to the research facility. Thus, pork samples handled by a controlled research technique and sampled at the point of slaughter are basically equivalent to what the consumer purchases at the retail level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1981.tb02978.x
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  • 7
    Electronic Resource
    Electronic Resource
    A cation-exchange study of stability constants of complexes formed between bismuth/III/ and nitrate or chloride ions (1990)
    Springer
    Journal of radioanalytical and nuclear chemistry 145 (1990), S. 167-173 
    Details
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract A cation-exchange method was used for determining the stability constants of complexes formed between Bi/III/, present in tracer concentrations /ca. 10−11M Bi/, and nitrate ion or chloride ion in 1.OM H/ClO4, NO3/ or 1.OM H/Cl, ClO4/ solutions, respectively. The successive formation constants were calculated using the distribution data. The resulting 1g β1, 1g β2, and 1g β3 values were 0.74, 1.22, and 1.54 for 1.OM H/ClO4, NO3/ solutions, and 2.36, 3.61, and 4.95 for 1.OM H/Cl, ClO4/ solutions, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02202021
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  • 8
    Electronic Resource
    Electronic Resource
    Monoclonal antibody against human bone alkaline phosphatase (1991)
    Springer
    International orthopaedics 15 (1991), S. 61-64 
    Details
    ISSN: 1432-5195
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Un anticorps monoclonal qui réagit préférentiellement avec les phosphatases alcalines (PA) de l'os humain a été obtenu en utilisant comme immunogène la PA osseuse d'un ostéosarcome humain. L'anticorps ayant la sélectivité la plus élevée présente une liaison trois fois et demie plus importante avec la PA osseuse qu'avec la PA hépatique. La sélectivité a été confirmée en utilisant des mélanges d'échantillons d'isoenzymes osseux et hépatiques. Sa sélectivité a été également démontrée par des mesures de la PA osseuse dans le sérum de patients présentant des maladies osseuses ou hépatiques. Un tel anticorps sélectif peut mener à obtenir un anticorps monoclonal hautement spécifique vis-à-vis de la PA osseuse, qui pourra être utilisé pour améliorer la sensibilité de l'analyse quantitative de l'isoenzyme.
    Notes: Summary A monoclonal antibody which reacts preferentially with human bone alkaline phosphatase (AP) has been developed using human osteosarcoma bone AP as an immunogen. The antibody with the highest selectivity shows about three- and a-half fold greater binding to bone than to liver AP. The selectivity was confirmed using mixtures of authentic samples of the bone and liver isoenzymes. Its selectivity was also shown by measurements of bone AP in sera from patients with either bone or liver diseases. Such a selective antibody could lead to the development of a monoclonal antibody highly specific for bone AP which can be used for a more sensitive approach for quantitative analysis of the isoenzyme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210537
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  • 9
    Electronic Resource
    Electronic Resource
    The enhancement of catecholamine-induced Cl− current by cyclic GMP revealed using photolabile caged compounds in guinea-pig ventricular cells (1993)
    Springer
    Pflügers Archiv 424 (1993), S. 546-548 
    Details
    ISSN: 1432-2013
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Whole cell currents were recorded in single myocytes dissociated from guinea-pig ventricles, and caged compounds were loaded intracellularly through the patch electrodes. Flash photolysis of caged cyclic GMP (cGMP) increased the amplitudes of both catecholamine-induced Cl− (ICl) and Ca2+ currents (ICa) which were pre-activated by submaximum doses of isoprenaline. Transient activation of ICl by photo-release of cyclic AMP (cAMP) showed a half decay time (t1/2) of 16.7±1.4 sec (mean±S.E.M., n=14). This decay was markedly delayed by inhibiting phosphodiesterases using 3-isobutyl-1-methyl-xanthine (IBMX). The intracellular application of cGMP (10–50μ M)also prolonged the decay of the ICl response to caged cAMP (t1/2=38.0±7.1 sec, n=12). These findings strongly support the hypothesis that cGMP facilitates theβ-adrenergic response of ionic currents through the inhibition of phosphodiesterase in mammalian cardiac myocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00374920
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  • 10
    Electronic Resource
    Electronic Resource
    Gamma-aminobutyric acid (GABA) immunoreactivity in the mouse adrenal gland (1993)
    Springer
    Histochemistry and cell biology 100 (1993), S. 203-213 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Gamma-aminobutyric acid (GABA) immunoreactivity was revealed by immunocytochemistry in the mouse adrenal gland at the light and electron microscopic levels. Groups of weakly or faintly GABA immunoreactive chromaffin cells were often seen in the adrenal medulla. By means of immunohistochemistry combined with fluorescent microscopy, these GABA immunoreactive chromaffin cells showed noradrenaline fluorescence. The immunoreaction product was seen mainly in the granular cores of these noradrenaline cells. These results suggest the co-existence of GABA and noradrenaline within the chromaffin granules. Sometimes thick or thin bundles of GABA immunoreactive nerve fibers with or without varicosities were found running through the cortex directly into the medulla. In the medulla, GABA immunoreactive varicose nerve fibers were numerous and were often in close contact with small adrenaline cells and large ganglion cells; a few, however, surrounded clusters of the noradrenaline cells, where membrane specializations were formed. Single GABA immunoreactive nerve fibers, and thin or thick bundles of the immunoreactive varicose nerve fibers ran along the blood vessels in the medulla. The immunoreaction deposits were observed diffusely in the axoplasm and in small agranular vesicles of the GABA immunoreactive nerve fibers. Since no ganglion cells with GABA immunoreactivity were found in the adrenal gland, the GABA immunoreactive nerve fibers are regarded as extrinsic in origin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269093
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