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  • 1990-1994  (4)
  • 1965-1969  (1)
  • 1
    ISSN: 1432-2307
    Keywords: Tubular basement membrane ; Peritubular capillary ; Cationic antigen ; Ovalbumin ; Trinitrophenol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We developed an experimental protocol for planting exogenous antigens with different molecular weights and charges on the constituents of the renal tubulointerstitium. The cationized antigens were injected selectively into the left renal arteries of Wistar rats. Antigen localization was documented by immunohistochemistry on frozen sections. Cationized bovine serum albumin (BSA; 68 kDa, isoelectric point =9.5) localized almost exclusively along the glomerular capillary wall. After application of highly cationic polyethyleneimine, cationized BSA given subsequently was found in a linear distribution along the glomerular capillary wall and along the peritubular capillaries. The fate of highly cationized ovalbumin conjugated with trinitrophenol (TNP-OA), subjected to gel filtration to obtain monomers (42 kDa, isoelectric point 〉10) differed; it was deposited in a linear pattern on the tubular basement membrane (TBM) and Bowman's capsule, and remained up to 36 h after injection. Noncationized, monomeric TNP-OA (42 kDa, isolectnic point =4.6) showed fine granular deposition in the tubular epithelium exclusively. These findings indicate that the barrier of the glomerular BM acts selectively on antigens with different molecular weights. They either settle on the peritubular capillaries, after passing the glomerular, or reach the urinary space, after which they are reabsorbed by the tubular epithelial cells to reach the TBM.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2307
    Keywords: Tubular basement membrane ; Peritubular capillary ; Cationic antigen ; Ovalbumin ; Trinitrophenol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We developed an experimental protocol for planting exogenous antigens with different molecular weights and charges on the constituents of the renal tubulointerstitium. The cationized antigens were injected selectively into the left renal arteries of Wistar rats. Antigen localization was documented by immunohistochemistry on frozen sections. Cationized bovine serum albumin (BSA; 68 kDa, isoelectric point =9.5) localized almost exclusively along the glomerular capillary wall. After application of highly cationic polyethyleneimine, cationized BSA given subsequently was found in a linear distribution along the glomerular capillary wall and along the peritubular capillaries. The fate of highly cationized ovalbumin conjugated with trinitrophenol (TNP-OA), subjected to gel filtration to obtain monomers (42 kDa, isoelectric point 〉10) differed; it was deposited in a linear pattern on the tubular basement membrane (TBM) and Bowman's capsule, and remained up to 36 h after injection. Noncationized, monomeric TNP-OA (42 kDa, isolectnic point =4.6) showed fine granular deposition in the tubular epithelium exclusively. These findings indicate that the barrier of the glomerular BM acts selectively on antigens with different molecular weights. They either settle on the peritubular capillaries, after passing the glomerular, or reach the urinary space, after which they are reabsorbed by the tubular epithelial cells to reach the TBM.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    European archives of psychiatry and clinical neuroscience 209 (1967), S. 450-461 
    ISSN: 1433-8491
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Zusammenfassung Von insgesamt 246 psychotischen Kranken wurden unter anderem auch 149 Schizophrene kinematographisch auf ihre mimischen Reaktionen hin getestet, zum Teil ist der Verlauf mit erfaßt. Die Besonderheiten der klinischen Gruppierungen mit deren jeweiliger Relation zu den Kretschmerschen Typen sind eingangs hervorgehoben. Leptosome und Dysplastiker traten zum Teil in „Kümmerformen“ auf; reine Pyknische waren seltener als Übergänge zum Dysplastischen. In 10% der Fälle ergaben sich abnorm symmetrische Gesichter von archaisch-infantiler Wirkung, in 20% auffällige Asymmetrien. — Hyperkinesen wie Grimassen, Faxen, Paramimien treten episodisch auf, es bleiben schließlich nur die Stereotypien, die sich vornehmlich in oberen Gesichtsbereichen abspielen; Ähnlichkeiten zu subcorticalen Hyperkinesen oder zum akinetischen Symptomenkomplex ergeben sich nicht; auch zu anderen Psychosen besteht mimisch keine Verwandschaft, bei exogenen Reaktionstypen ist allerdings eine Abgrenzung aus dem Zustandsbild allein nicht immer möglich, — Im Verlauf zerfällt die Mimik Schizophrener, wobei Ausgangstyp, Intensität und Dauer eine Rolle spielen; am ungünstigsten verhalten sich dabei „einfache Verläufe“ an Dysplastikern. Die Veränderung beginnt meist oral mit Innervationsunsicherheit der Lippen; in sieben Fällen war dabei ein halbseitiges Überwiegen nach Art einer „mimischen Parese“ festzustellen. Die Züge können völlig erschlaffen, was jedoch Ausnahmen sind; meist bleibt eine Überspannung in frontoorbitalen Bereichen zurück, wie man es ähnlich bei hirnatrophischen Prozessen findet; im Unterschied zu letzteren sind die atonischen unteren Gesichtsbereiche bei Schizophrenen jedoch rein motorisch intakt, dafür zeigt sich hier aber ein ungraziöses, seelenloses mimisches Verhalten, was Verfasser als thalamisches Syndrom irreversibler Natur deuten möchte.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 3Y1 rat fibroblasts transformed by adenovirus type 12 or by its E1A gene formed syncytia when cocultivated with the Friend as well as other ecotropic murine leukemia-virus-producing cells. In contrast, the parental 3Y1 cells and those transformed by other tumor viruses or by a chemical carcinogen exhibited no syncytium formation.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Rat 3Y1 fibroblasts transformed by adenovirus type 12 or its E1A gene formed syncytia by cocultivation with Friend murine leukemia virus (MuLV)-producing cells. On the other hand, parental 3Y1 cells and those derivatives induced by other tumor viruses or chemical carcinogen showed no MuLV-mediated syncytium formation [N. Momozaki et al. (1990) Arch. Virol. 115: 123–126]. The expression of major histocompatibility complex (MHC) class I mRNA and antigens was significantly reduced in these Ad12- and E1A-transformed 3Y1 cells. In contrast, other tumor virus- and chemical carcinogen-transformed 3Y1 cells expressed MHC class I almost in normal levels as did parental 3Y1 cells. Furthermore, Ad12-transformed 3Y1 cells which started to express the transfected exogenous MHC class I gene,H-2L d, showed no more MuLV-mediated 3Y1 cell fusion. These results indicate that the expression of MHC class I on the cell membrane is closely related to the inhibition of 3Y1 cell fusion by MuLV.
    Type of Medium: Electronic Resource
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