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  • 1
    Electronic Resource
    Electronic Resource
    IDENTIFICATION OF A RECOMBINATIONAL SIGNAL SEQUENCE-SPECIFIC DNA-BINDING PROTEIN(S) OF Mr 115,000 IN THE NUCLEAR EXTRACTS FROM IMMATURE LYMPHOID CELL LINES (1990)
    Oxford, UK : Blackwell Publishing Ltd
    International journal of immunogenetics 17 (1990), S. 0 
    Details
    ISSN: 1744-313X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Rearrangements of immunoglobulin genes are mediated by highly conserved heptamer and nonamer recombinational signal sequence. Using a protein-blotting procedure, a heptamer and nonamer recombinational signal sequence-specific DNA-binding protein(s) was examined in the nuclear extracts from lymphoid and nonlymphoid cell lines. Nuclear extracts were subjected to SDS-polyacrylamide gel electrophoresis, and transferred by electroblotting to nitrocellulose filters. Then the filters were hybridized to *P-labelled synthetic double-stranded heptamer-23bp-nonamer or nonamer- l2bp-heptamer recombinational signal sequence probes. A relatively large amount of a DNA-binding protein(s) of M, 115,000 for both probes was detected in the nuclear extracts from immature B and immature T cell lines. No DNA-binding proteins were detected in a myeloma cell line. Interestingly, this DNA-binding protein(s) might be able to recognize both heptamer and nonamer. Recombinational signal sequence-specific DNA-binding activity of the protein(s) and the presence of the protein(s) in a stage-specific manner strongly suggest that the protein(s) of M, 115,000 detected here may play an important role in the recombination of Ig and TCR genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1744-313X.1990.tb00860.x
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  • 2
    Electronic Resource
    Electronic Resource
    Charged particle densities and kinetics in a radio-frequency SF6 plasma (1994)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 76 (1994), S. 7221-7230 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Electron, negative ion, and positive ion densities in a capacitively-coupled radio-frequency (rf, 13.56 MHz) SF6 plasma have been investigated as functions of the pressure (30–700 mTorr) and rf power. The decay of the charged particle densities in the afterglow has also been studied to obtain information about their kinetics. The electron density was determined by using a microwave cavity resonance technique. Negative ions were detected by measuring the density of photodetached electrons produced by pulsed laser irradiation of the plasma. The positive ion density was obtained from Langmuir probe measurements. At a rf power of 0.13 W/cm2 and at low pressures ((approximately-less-than)100 mTorr) the positive ion density, which is virtually equal to the negative ion density, is found to be larger than the electron density by a factor of a few hundred; the ion/electron density ratio increases with increasing pressure to reach a value of a few thousand at high pressures. Wavelength-dependent photodetachment measurements indicate that the dominant contribution to the photodetachment signal at 266 nm comes from F−, but F− is a minor negative-ion species with respect to the density; that is, the photodetachment efficiency for the dominant negative ion species (supposed to be SF−5) is low. The recombination rate constant for F− (supposedly with SF+5) is suggested to be an order of magnitude larger than that for the dominant ion species, the latter being estimated to be slightly smaller than 10−7 cm3 s−1. © 1994 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.358003
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  • 3
    Electronic Resource
    Electronic Resource
    X-ray area detector based on CCD readout for diffraction study (abstract) : Proceedings of the 4th International conference on synchrotron radiation instrumentation (1992)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 63 (1992), S. 659-659 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: An x-ray area detector based on charged coupled device (CCD) readout has been designed and constructed for diffraction studies. Incident x-rays are converted to visible photons by a phosphor screen [Y2O2S or CsI(Na)] which is attached via fiber optic to the input surface of the demagnifying image intensifier with a 4:1 demagnification. The intensified visible image created on the output phosphor screen of the image intensifier is viewed through a 1:1 optical lens system by the cooled CCD (Thomson:THX31156) which has 1024×1024 pixels (pixel size: 19 μm×19 μm). The detector system should have the following characteristics: active area: 100 mm in diameter, spatial resolution: 0.1 mm×0.1 mm, detective quantum efficiency (DQE): about 70% for 8 keV, dynamic range: more than 104, readout time: 4 s. Nonuniformity of response due to the image intensifier is corrected by software. The CCD is cooled to −30° to −40° to reduce the dark current. The background noise level of the detector system corresponds to less than 10 x-ray photons per pixel. Detailed hardware and software configuration and measured performance of the system will be described. The system will be applied to small-angle x-ray scattering (SAXS) and protein crystallography.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1142682
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  • 4
    Electronic Resource
    Electronic Resource
    Inhibitory Action of CCK-OP on Rat Proximal Colon (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 713 (1994), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1994.tb44109.x
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  • 5
    Electronic Resource
    Electronic Resource
    Spontaneous activation of heat shock protein gene transcription in peripheral blood mononuclear cells of individuals with active systemic lupus erythematosus (1990)
    Springer
    Clinical rheumatology 9 (1990), S. 182-185 
    Details
    ISSN: 1434-9949
    Keywords: Heat Shock Protein ; Gene Transcription ; Systemic Lupus Erythematosus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In the present study, we show the spontaneous elevation of the transcription of heat shock protein (hsp 70) gene in peripheral blood mononuclear cells (PBMC) of individuals with active systemic lupus erythematosus (SLE) by nuclear run-on transcription assay. For quantification analysis of hsp 70 gene transcription, we first found more than ten times of the enhanced transcription of hsp 70 gene in PBMC of individuals with active SLE, as compared to normal healthy subjects or patients with bronchial asthma as controls. Our observation suggests that heat shock proteins may be produced during an active immune response in individuals with active systemic lupus erythematosus and is involved in lupus-related change of possible essential function in PBMC.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02031966
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  • 6
    Electronic Resource
    Electronic Resource
    Antidromically evoked facial nerve responses in guinea pigs: a basis for clinical applications in patients with facial palsy (1990)
    Springer
    European archives of oto-rhino-laryngology and head & neck 247 (1990), S. 151-155 
    Details
    ISSN: 1434-4726
    Keywords: Antidromic facial nerve response ; Facial paralysis ; Guinea pig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We investigated the differences in wave form in antidromically evoked facial nerve responses in guinea pigs with and without facial nerve dysfunction. The antidromic facial nerve responses were evoked with alternative stimulation of positive and negative square-wave pulses of 0.1 ms duration and recorded at the bony fallopian canal near the geniculate ganglion. One hundred responses were summed by a signal processor. The application of alternative stimulations made it possible to eliminate stimulus artifacts and to analyze precisely the waves with latencies shorter than 0.5 ms in the test animals. Normal antidromic facial nerve responses showed a triphasic wave form with two positive and one negative peaks. A blockade of the nerve between the recording and stimulating sites resulted in transformation of the wave into a monophasic one. A proximal blockade to the recording site changed the wave form to a biphasic shape. These findings suggest that the site of an intratemporal facial nerve lesion can be predicted from the wave forms evoked by antidromic facial nerve responses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00175966
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  • 7
    Electronic Resource
    Electronic Resource
    Growth advantage by overexpression of normal Harvey ras proto-oncogene in cultured rat epidermal keratinocytes (1990)
    Springer
    Archives of dermatological research 282 (1990), S. 330-334 
    Details
    ISSN: 1432-069X
    Keywords: Harvey ras proto-oncogene ; Gene expression ; Keratinocytes ; Cell growth control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The ras proto-oncogene is frequently amplified and overexpressed in the hyperproliferative conditions of epidermal keratinocytes. To investigate the effects of its overexpression on the growth of keratinocytes in a model system, we constructed expression vectors for normal human Ha-ras and introduced them into FRSK cells, a fetal rat epidermal keratinocyte cell line. Several clones containing the transfected Ha-ras were isolated, and two of them overexpressed this gene. In these clones DNA synthesis and cell growth were greater than in other clones expressing this gene at low levels. Thus we suggest that overexpression of normal ras gene may provide growth advantage to epidermal keratinocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00375728
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  • 8
    Electronic Resource
    Electronic Resource
    Enhancement of surface acidity and catalytic activity by glass formation of calcium metaphosphate (1991)
    Springer
    Catalysis letters 11 (1991), S. 49-53 
    Details
    ISSN: 1572-879X
    Keywords: Calcium metaphosphate glass ; surface acidity ; catalytic activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Effective acid sites for the dehydration of 2-propanol and isomerization of 1-butene were found to be generated by glass formation of calcium metaphosphate. The number of these sites with strong acidity remarkably decreased by the crystallization of the glass, where the condensation of hydroxyl groups were significant. Thus, the sites must be the monohydrogen phosphate protons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00866900
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  • 9
    Electronic Resource
    Electronic Resource
    Effect of hydrogen on carbon deposition from carbon monoxide on nickel catalyst (1994)
    Springer
    Catalysis letters 23 (1994), S. 237-243 
    Details
    ISSN: 1572-879X
    Keywords: Carbon deposition ; hydrogen effect ; nickel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The role of hydrogen in carbon deposition on Ni has been studied at H2/CO 〈 1 and 698 K by determining the respective rates of the carbon-forming reactions: (1) CO + H2 →-C + H2O and (2) 2CO → C + CO2. The steady-state rate of reaction (1) increases in proportion to H2 pressure. On the other hand, reaction (2) is facilitated by the addition of an extremely small amount of H2, so that the rate becomes about eight times that for pure CO but hardly varies as more H2 is added. Similarly, there is a great difference in catalytic activity for ethylene hydrogenation between spent catalysts obtained in the deposition with and without H2. These findings suggest that hydrogen, even in a small amount, makes free Ni surface area larger than for pure CO.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00811358
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  • 10
    Electronic Resource
    Electronic Resource
    Nuclear Resonant scattering experiments with synchrotron radiation at KEK (1992)
    Springer
    Hyperfine interactions 71 (1992), S. 1491-1494 
    Details
    ISSN: 1572-9540
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Experiments of nuclear Resonant scattering carried out at PF and TRISTAN-AR of KEK are described, which include nuclear Bragg scattering in time domain as well as energy domain, time-resolved nuclear resonant forward scattering, and interferometric studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02397365
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