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  • 1
    Electronic Resource
    Electronic Resource
    Distribution of neuropeptide Y in the prosencephalon of man and Cotton-head Tamarin (Saguinus oedipus): colocalization with somatostatin in neurons of striatum and amygdala (1990)
    Springer
    Anatomy and embryology 181 (1990), S. 157-166 
    Details
    ISSN: 1432-0568
    Keywords: Neuropeptide Y ; Human brain ; Immunocytochemistry ; High performance liquid chromatography ; Colocalization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The presence, chromatographic properties and localization of neuropeptide Y was demonstrated in postmortem human brain areas of neurologically and neuropsychiatrically normative controls using immunocytochemistry and high performance liquid chromatography combined with radioimmunoassay. NPY-immunoreactivity was found in many regions of the prosencephalon. Numerous perikarya and fibers were present in the neocortex, basal ganglia and limbic-hypothalamic areas. A moderate number of neurons and fibers was observed in the basal forebrain, including the septal complex. A comparative immunohistochemical investigation in perfusion-fixed brains of the old-world ape Saguinus oedipus revealed an almost identical distribution of NPY-immunoreactivity with only minor differences. Colocalization experiments on 1–2 μm thin consecutive paraffin sections revealed a large number of NPY neurons throughout the human neostriatum and amygdaloid complex that were also positive for somatostatin. Our findings indicate that detection of neuropeptides in fresh or fixed post-mortem human tissue by different immunochemical methods may actually reflect the in vivo conditions. In addition, the wide distribution of NPY throughout the human brain and its colocalization with other neurotransmitters suggests a physiological role as neuroactive substance, i.e. neuromodulator in the primate central nervous system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00198955
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  • 2
    Electronic Resource
    Electronic Resource
    Visualization of lectin-like proteins in human placenta by means of anti-plant lectin antibodies (1993)
    Springer
    Anatomy and embryology 187 (1993), S. 465-473 
    Details
    ISSN: 1432-0568
    Keywords: Placenta ; Trophoblast ; Immunocytochemistry ; Gel electrophoresis ; Immunochemistry ; Lectin activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Proteins antigenically cross-reactive with lectins were sought in the placenta by immunohistochemistry using polyclonal antibodies raised in rabbit against four well-known lectins: Concanavalin A, Wheat germ agglutinin, Ulex europaeus agglutinin, and Phaseolus vulgaris leukoagglutinin (PHA-L), as well as one antibody raised in goat against PHA-L. Even at high dilutions of the primary antibody, strong staining was obtained after short incubations, in patterns generally resembling those obtained for placental lectins by other means, such as those based on binding capacity for glycosylated probes. One of the immunohistochemical patterns distinguishes with great clarity between the trophoblast cell layers, thus relating to developmental and functional parameters; another localises PHA-L-immunoreactivity to the syncytiotrophoblast. These results underline the validity of the immunohistochemical screening as an approach in its own right. Both positive and negative controls were applied to the immunohistochemical methodology. These controls showed that the staining patterns obtained relate to the specificities of the primary antibodies employed; i.e. to lectins. The PHA-Llike cross-reactivity was analysed immunochemically. In electrophoretically separated and Western-blotted placental extracts there were found anti-PHA-L-binding fractions of apparent molecular weights 30 kDa, 58 kDa and 67 kDa. Control studies of the PHA-L antigen showed anti-PHA-L-binding fractions of approximate molecular weights 32 kDa and 60 kDa. The 30 kDa fraction from placenta and the 32 kDa fraction from PHA-L antigen bound lactosylated BSA but not fucosylated BSA. Taken together, the immunohistochemical and biochemical data reveal the presence in the placenta of lectins, one of which resembles PHA-L not only antigenically but also in molecular weight and in sugar-binding specificity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00174422
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  • 3
    Electronic Resource
    Electronic Resource
    DX-Centers in Silicon δ-Doped GaAs and Al〈sub〉x〈/sub〉Ga〈sub〉1-x〈/sub〉As? (1991)
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 65-66 (Jan. 1991), p. 461-466 
    Details
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/02/20/transtech_doi~10.4028%252Fwww.scientific.net%252FMSF.65-66.461.pdf
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  • 4
    Electronic Resource
    Electronic Resource
    Chromosomal Assignment of Three Enzyme Coding Loci (Icd1, Nad-Mdh1 and Aco1) Using Primary Trisomics in Beet (Beta vulgaris L.) (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 111 (1993), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Chromosomal assignment of three enzyme coding loci was established by evaluation of the segregation distortion in the offspring of crosses between heterozygous primary trisomics of a complete series in beet (Beta vulgaris) and heterozygous diploid pollinators. Depending on the rate of isozyme polymorphism in the original plant material, at least one, but more often two, crossing cycles were needed to obtain the desired segregating populations. In one case, a backcross was used to confirm the segregation distortion, and in two cases, the chromosomal assignment of the isozyme loci was confirmed by studying the dosage shift in the electrophoretic pattern of the critical trisomics. The isocitrate dehydrogenase locus (Icd1) is situated on chromosome II, the NAD-dependent malate dehydrogenase locus (Nad-Mdh1) on chromosome III, and the aconitase locus (Aco1) on chromosome IV.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1993.tb00627.x
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  • 5
    Electronic Resource
    Electronic Resource
    Resistance to Polymyxa betae in Beta Species of the Section Procumbentes, in Hybrids with B. vulgaris and in Monosomic Chromosome Additions of B. procumbens in B. vulgaris (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 109 (1992), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Resistance to Polymyxa betae was studied in Beta species of the section Procumbentes, in hybrids of B. vulgaris with B. procumbens or B. patellaris, and in monosomic additions of chromosomes of B. procumbens in B. vulgaris. In all experiments P. betae infested with beet necrotic yellow vein virus (BNYVV) was used. This virus causes rhizomania in sugar beet, and the effect of vector resistance was studied by measuring virus concentrations. Cystosori of P. betae were not found in the wild species and the hybrids. Virus concentrations in these plants were low, and in half the number of plants the virus could not be detected. Results of experiments with the monosomic additions indicate, that resistance to P. betae in B. procumbens is located on chromosomes 4 and 8. Some cystosori were present in these addition types, while cystosori were abundantly present in other addition types and all sib-plants. Virus concentrations in the addition types 4 and 8 were lower than in their sib-plants, but in almost all plants the virus could be detected. A significant correlation (r = 0.91; P 〈 0.05) between average numbers of cystosori and average virus concentrations was found when addition families of type 8 were tested together with B. procumbens and B. vulgaris cv. ‘Regina’.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1992.tb00184.x
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  • 6
    Electronic Resource
    Electronic Resource
    Peptidergic innervation within the prostate gland and seminal vesicle (1990)
    Springer
    Urological research 18 (1990), S. 337-340 
    Details
    ISSN: 1434-0879
    Keywords: Neuropeptides ; Prostate gland ; Seminal vesicle ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary An immunohistochemical study in which antisera against several neuropeptides were used demonstrated the presence of neuropeptide Y(NPY) and vasoactive intestinal polypeptide (VIP) immunoreactivity in nerve fibers in the human prostate gland and seminal vesicle, whereas no immunostaining for substance P and calcitonin gene-related peptide was observed. The peptidergic innervation was found to be generally moderate to low. NPY-and VIP-immunoreactive fibers were localized in the subepithelial connective tissue as well as the smooth muscle layers in both organs, although the peptidergic fiber networks were more prominent in the seminal vesicle. Most NPY-immunoreactive fibers were observed in the musculature of the seminal vesicle.In addition, NPY-and VIP-immunoreactive fibers were demonstrated in the walls of blood vessels. The results of our study suggest that the innervation of the prostate gland and seminal vesicle by various neuroactive peptides may be involved in the autonomic regulation of these organs in adult man, as well as sympathetic and parasympathetic nerve fibers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00300783
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  • 7
    Electronic Resource
    Electronic Resource
    Isozyme analysis of primary trisomies in beet (Beta vulgaris L.). Genetical characterization and techniques for chromosomal assignment of two enzyme-coding loci: leucine aminopeptidase and glutamate oxaloacetate transaminase (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 761-768 
    Details
    ISSN: 1432-2242
    Keywords: Primary trisomies ; Beet ; Beta vulgaris ; Isozyme polymorphism ; Chromosomal assignment ; Distorted segregation ; Dosage shift
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Segregating families of beet (Beta vulgaris) were used to verify the monofactorial inheritance of two enzyme-coding loci, leucine aminopeptidase (Lap1) and glutamate oxaloacetate transaminase (Got3). With a series of primary trisomies and using three methods to discriminate between the critical trisomic (the locus is situated on the triplicated chromosome) and the non-critical ones, it was possible to allocate the two loci to beet chromosomes I and II, respectively. For the locus Lap1 distorted segregation ratios were estimated, and the incorporation of three alleles into one plant was attempted. In the case of Got3 the measurement of the allele dosage effect after electrophoresis was chosen as the major strategy. The output of laser densitometric scans were subjected to the non-parametrical Wilcoxon-Mann-Whitney test.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222667
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  • 8
    Electronic Resource
    Electronic Resource
    Long-range organization of a satellite DNA family flanking the beet cyst nematode resistance locus (Hs1) on chromosome-1 of B. patellaris and B. procumbens (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 459-466 
    Details
    ISSN: 1432-2242
    Keywords: Molecular markers Beet cyst nematode resistance ; Hs1 Heterodera schachtii Schm ; Satellite DNA Monosomic fragment additions Beta ; Pulsed-field gel electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract New members of a satellite DNA family (Sat 121), specific for wild beets of the section Procumbentes of the genus Beta, were isolated. Sequence analysis showed that the members of Sat-121 fall into two distinct classes. The organization of Sat-121 in the vicinity of a beet cyst nematode (Heterodera schachtii Schm.) resistance locus (Hs1) in B. patellaris and B. procumbens was investigated by pulsed-field gel electrophoresis (PFGE) using DNA from a series of resistant monosomic fragment additions, each containing an extra chromosome fragment of B. patellaris chromosome-1 (pat-1) in B. vulgaris. In this way several clusters of Sat-121 flanking the Hs1 pat-1 locus were identified. In nematode resistant diploid introgressions (2n=18), which contain small segments of B. procumbens chromosome-1 (pro-1) in B. vulgaris, only two major Sat-121 clusters were detected near the Hs1 pro-1 locus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225381
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  • 9
    Electronic Resource
    Electronic Resource
    Apparent decrease and elimination of BCR/ABL mRNA-expressing residual cells in patients with chronic myelogenous leukemia after allogeneic bone marrow transplantation (1991)
    Springer
    Annals of hematology 63 (1991), S. 189-194 
    Details
    ISSN: 1432-0584
    Keywords: Chronic myelogenous leukemia ; Allogeneic bone marrow transplantation ; Minimal residual disease ; BCR/ABL mRNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A modified two-step polymerase chain reaction (PCR) was used for the amplification of BCR/ABL mRNA in 16 patients with Philadelphia chromosomepositive (Ph+) chronic myelogenous leukemia (CML) following allogeneic bone marrow transplantation (BMT). At different intervals after BMT, patient cells were assessed for the presence of BCR/ABL mRNA by two subsequent rounds of PCR amplification; this procedure increased the sensitivity for the detection of one Ph+ cell in 104–5 to one cell in 105–6. Eight of 16 patients were negative by two-step PCR 1–39 months after BMT, suggesting an elimination of Ph-positive cells or a decrease below the threshold of detection. Although five patients showed negative results by the one-step PCR only, they were tested positive when nested primers were used, indicating a substantial decrease in the amount of BCR/ABL target mRNA compared with earlier pre- or post-transplant analyses. One patient who was still PCR positive 27 months after BMT became negative 12 months later. Persistence of BCR/ABL mRNA-expressing cells correlated with subsequent clinical relapse only when the transplantation was performed during blast crisis. All patients who underwent transplantation in chronic phase, including those with BCR rearrangement by PCR, are in clinical and hematological remission between 24 and 95 months after BMT. We conclude that aggressive chemotherapy combined with total body irradiation is unable to completely eradicate the malignant clone in all CML patients, and it might be speculated that other mechanisms (e.g., graft versus host reaction [GVHD] or graft versus leukemia effect [GVL]) may effectively eliminate residual leukemic cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01703441
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  • 10
    Electronic Resource
    Electronic Resource
    Medullary histiocytosis following treatment of severe aplastic anemia with recombinant human interleukin-3 in combination with antilymphocyte globulin, cyclosporin A, and methylprednisolone (1991)
    Springer
    Annals of hematology 63 (1991), S. 229-231 
    Details
    ISSN: 1432-0584
    Keywords: Aplastic anemia ; Therapy ; Interleukin-3
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary This case report describes the clinical use of recombinant human interleukin-3 as adjunct to immunosuppressive therapy with antilymphocyte globulin, cyclosporin A, and methylprednisolone for refractory severe aplastic anemia. Hematopoietic response to treatment was moderate and peripheral blood counts (neutrophils, eosinophils, monocytes, reticulocytes) increased only slightly. Unexpectedly, during the time of interleukin-3 administration a substantial bone marrow infiltration by macrophages became detectable, consistent with the diagnosis of medullary histiocytosis, that may have prevented recovery of normal hematopoiesis in this patient. This observation may indicate the need for careful use of interleukin-3 in patients with drug-induced immunodeficiency.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01703450
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