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  • 1
    Electronic Resource
    Electronic Resource
    Postnatal development of neuropeptide Y- and calcitonin gene-related peptide-immunoreactive nerves in the rat urinary bladder (1994)
    Springer
    Anatomy and embryology 189 (1994), S. 361-373 
    Details
    ISSN: 1432-0568
    Keywords: Postnatal development ; Neuropeptide Y ; Calcitonin gene-related peptide ; Urinary bladder ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The postnatal development of neuropeptide Y- and calcitonin gene-related peptide-immunoreactive (NPY-IR and CGRP-IR) nerve fibers in the rat urinary bladder was investigated using whole-mount preparations and cryostat sections. In newborn and 3-day-old rats, many NPY-IR nerve fibers were observed in the subserous and muscle layers. Many NPY-IR nerve cell bodies clustered at branching points of the subserous nerve bundles. Within 4 weeks after birth, these cell bodies drastically decreased in number and spread along the bundles, although the number of NPY-IR nerve fibers increased moderately. In contrast, CGRP-IR nerve fibers in newborn and 3-day-old rats were less developed, and no CGRP-IR nerve cell body was observed in any rat. However, CGRP-IR nerve fiber distribution in the urinary tissues conspicuously increased within 4 weeks after birth. Especially, an increase of the infraepithelial fibers showing a meshwork appearance was prominent in the fundus and corpus of the bladder. The infra- and intraepithelial CGRP-IR nerve meshwork of the ventral wall was more dense than that of the trigone. At 4 weeks, NPY-IR and CGRP-IR nerves were similar to those of the adult rat (8–12 weeks old). The present study suggests a correlation between the development of the peripheral nervous system in the urinary bladder and maturation of micturition behavior in the rat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00190591
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  • 2
    Electronic Resource
    Electronic Resource
    A cation-exchange study of stability constants of complexes formed between bismuth/III/ and nitrate or chloride ions (1990)
    Springer
    Journal of radioanalytical and nuclear chemistry 145 (1990), S. 167-173 
    Details
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract A cation-exchange method was used for determining the stability constants of complexes formed between Bi/III/, present in tracer concentrations /ca. 10−11M Bi/, and nitrate ion or chloride ion in 1.OM H/ClO4, NO3/ or 1.OM H/Cl, ClO4/ solutions, respectively. The successive formation constants were calculated using the distribution data. The resulting 1g β1, 1g β2, and 1g β3 values were 0.74, 1.22, and 1.54 for 1.OM H/ClO4, NO3/ solutions, and 2.36, 3.61, and 4.95 for 1.OM H/Cl, ClO4/ solutions, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02202021
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  • 3
    Electronic Resource
    Electronic Resource
    Monoclonal antibody against human bone alkaline phosphatase (1991)
    Springer
    International orthopaedics 15 (1991), S. 61-64 
    Details
    ISSN: 1432-5195
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Un anticorps monoclonal qui réagit préférentiellement avec les phosphatases alcalines (PA) de l'os humain a été obtenu en utilisant comme immunogène la PA osseuse d'un ostéosarcome humain. L'anticorps ayant la sélectivité la plus élevée présente une liaison trois fois et demie plus importante avec la PA osseuse qu'avec la PA hépatique. La sélectivité a été confirmée en utilisant des mélanges d'échantillons d'isoenzymes osseux et hépatiques. Sa sélectivité a été également démontrée par des mesures de la PA osseuse dans le sérum de patients présentant des maladies osseuses ou hépatiques. Un tel anticorps sélectif peut mener à obtenir un anticorps monoclonal hautement spécifique vis-à-vis de la PA osseuse, qui pourra être utilisé pour améliorer la sensibilité de l'analyse quantitative de l'isoenzyme.
    Notes: Summary A monoclonal antibody which reacts preferentially with human bone alkaline phosphatase (AP) has been developed using human osteosarcoma bone AP as an immunogen. The antibody with the highest selectivity shows about three- and a-half fold greater binding to bone than to liver AP. The selectivity was confirmed using mixtures of authentic samples of the bone and liver isoenzymes. Its selectivity was also shown by measurements of bone AP in sera from patients with either bone or liver diseases. Such a selective antibody could lead to the development of a monoclonal antibody highly specific for bone AP which can be used for a more sensitive approach for quantitative analysis of the isoenzyme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210537
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  • 4
    Electronic Resource
    Electronic Resource
    The enhancement of catecholamine-induced Cl− current by cyclic GMP revealed using photolabile caged compounds in guinea-pig ventricular cells (1993)
    Springer
    Pflügers Archiv 424 (1993), S. 546-548 
    Details
    ISSN: 1432-2013
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Whole cell currents were recorded in single myocytes dissociated from guinea-pig ventricles, and caged compounds were loaded intracellularly through the patch electrodes. Flash photolysis of caged cyclic GMP (cGMP) increased the amplitudes of both catecholamine-induced Cl− (ICl) and Ca2+ currents (ICa) which were pre-activated by submaximum doses of isoprenaline. Transient activation of ICl by photo-release of cyclic AMP (cAMP) showed a half decay time (t1/2) of 16.7±1.4 sec (mean±S.E.M., n=14). This decay was markedly delayed by inhibiting phosphodiesterases using 3-isobutyl-1-methyl-xanthine (IBMX). The intracellular application of cGMP (10–50μ M)also prolonged the decay of the ICl response to caged cAMP (t1/2=38.0±7.1 sec, n=12). These findings strongly support the hypothesis that cGMP facilitates theβ-adrenergic response of ionic currents through the inhibition of phosphodiesterase in mammalian cardiac myocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00374920
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  • 5
    Electronic Resource
    Electronic Resource
    Gamma-aminobutyric acid (GABA) immunoreactivity in the mouse adrenal gland (1993)
    Springer
    Histochemistry and cell biology 100 (1993), S. 203-213 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Gamma-aminobutyric acid (GABA) immunoreactivity was revealed by immunocytochemistry in the mouse adrenal gland at the light and electron microscopic levels. Groups of weakly or faintly GABA immunoreactive chromaffin cells were often seen in the adrenal medulla. By means of immunohistochemistry combined with fluorescent microscopy, these GABA immunoreactive chromaffin cells showed noradrenaline fluorescence. The immunoreaction product was seen mainly in the granular cores of these noradrenaline cells. These results suggest the co-existence of GABA and noradrenaline within the chromaffin granules. Sometimes thick or thin bundles of GABA immunoreactive nerve fibers with or without varicosities were found running through the cortex directly into the medulla. In the medulla, GABA immunoreactive varicose nerve fibers were numerous and were often in close contact with small adrenaline cells and large ganglion cells; a few, however, surrounded clusters of the noradrenaline cells, where membrane specializations were formed. Single GABA immunoreactive nerve fibers, and thin or thick bundles of the immunoreactive varicose nerve fibers ran along the blood vessels in the medulla. The immunoreaction deposits were observed diffusely in the axoplasm and in small agranular vesicles of the GABA immunoreactive nerve fibers. Since no ganglion cells with GABA immunoreactivity were found in the adrenal gland, the GABA immunoreactive nerve fibers are regarded as extrinsic in origin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269093
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  • 6
    Electronic Resource
    Electronic Resource
    Immunocytochemical study of tyrosine hydroxylase and dopamine β-hydroxylase immunoreactivities in the rat pancreas (1994)
    Springer
    Histochemistry and cell biology 101 (1994), S. 313-323 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract An immunohistochemical and immunoelectron microscopic study was used to demonstrate tyrosine hydroxylase (TH) and dopamine β-hydroxylase (DBH) immunoreactivities in the rat pancreas. Small TH immunoreactive cells were found in close contact with large TH immunonegative ganglion cells among the exocrine glands and were occasionally found in some islets. Some of these TH immunoreactive cells were also DBH immunopositive. The immunoreaction product was seen diffusely in the cytoplasm and in the granule cores of TH immunoreactive cells. All intra-pancreatic ganglion cells were immunoreactive for DBH, but not for TH. The TH immunoreactive cells were identified as small intensely fluorescent (SIF) cells due to their localization and morphological characteristics and showed no insulin, glucagon, somatostatin or pancreatic polypeptide immunoreactivities. These results indicate that SIF cells may release dopamine or noradrenaline to adequate stimuli while the intra-pancreatic ganglion cells with only DBH may not synthesize catecholamines in a normal biosynthetic pathway. TH immunoreactive nerve bundles without varicosities and fibers with varicosities, associated or unassociated with blood vessels, were found in both the exocrine and endocrine pancreas. Close apposition of TH immunoreactive nerve fibers to the smooth muscle and endothelial cells of the blood vessels was observed. A close apposition between TH immunoreactive nerve fibers and exocrine acinar cells and islet endocrine cells was sometimes found in the pancreas. The immunoreaction product was seen diffusely in the axoplasm and in the granular vesicles of the immunoreactive nerve fibers. Since no TH immunoreactive ganglion cells were present in the rat pancreas, the present study suggests that noradrenergic nerve fibers in the pancreas may be extrinsic in origin, and may exert an effect on the regulation of blood flow and on the secretory acitivity of the acinar cells, duct cells and endocrine cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268992
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  • 7
    Electronic Resource
    Electronic Resource
    Alumina ceramic prostheses for bone tumor surgery (1992)
    Springer
    Archives of orthopaedic and trauma surgery 112 (1992), S. 1-4 
    Details
    ISSN: 1434-3916
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Between 1979 and 1990 reconstruction using a ceramic prosthesis with a polycrystal alumina segment and a monocrystal alumina stem was carried out in 65 patients after the resection of malignant or benign aggressive bone tumors. Resection of 18 osteosarcomas, 5 chondrosarcomas, 9 other sarcomas, 10 giant cell tumors, 20 metastatic bone tumors, and 3 other bone tumors was followed by replacement of 17 proximal femurs, 12 distal femurs, 12 proximal tibia, 11 proximal humeri, 3 distal radii, 5 midshafts of the long bone, 2 pelvises, and 3 other parts. Results were rated excellent in 4 cases, good in 43, fair in 13, and poor in 4. In the cases with benignly aggressive or low-grade malignant tumors and those with tumors of the proximal femur, proximal tibia, or midshaft, satisfactory results can be obtained. Four skin ulcers, three dislocations, three loosenings, two infections, and two breaks were noted. Close interfacing between the ceramic prosthesis and the bone was observed radiologically in all cases with cementless fixation except in cases with high-grade malignancies in the knee joint. These results demonstrate that the ceramic prosthesis can be beneficial for the management of patients with benignly aggressive or low-grade malignant bone tumors who have retained adequate muscle strength around the joint even after tumor resection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00431035
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  • 8
    Electronic Resource
    Electronic Resource
    A new model of osteopenia using ectopically induced bone: effects of parathyroid hormone and estradiol on bone turnover in induced bone (1993)
    Springer
    Osteoporosis international 3 (1993), S. 128-131 
    Details
    ISSN: 1433-2965
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01621886
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  • 9
    Electronic Resource
    Electronic Resource
    Molecular cloning of feline leukemia provirus genomes integrated in the feline large granular lymphoma cells (1990)
    Springer
    Archives of virology 111 (1990), S. 177-185 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Feline leukemia virus (FeLV) is a horizontally transmitted agent of the domestic cat which is known to be associated with wide spectrum of diseases of the hematopoietic system. In the present study, proviral DNAs of FeLV proviruses were examined in the tumor cells of natural killer cell lineage which is very rare in cats. In the chromosomal DNA of the tumor cells, 5 distinct bands corresponding to exogenous FeLV provirus genomes were detected by digestion withEcoRI which does not cut most FeLV isolates. Five clones of pLC1, pLC2, pLC3, pLC4, and pLC5 obtained from the 5 respective bands were analysed by restriction endonuclease mapping and Southern blot hybridization using gene-specific probes of FeLV. The results have clearly demonstrated that pLC4 and pLC5 contained large deletions in thepol and part ofgag regions, while the full-length proviruses could be observed in pLC1 and pLC2. Furthermore, pLC3 contained part of a variant FeLV genome having anEcoRI site in itsgag region. The molecular clones of defective and variant FeLV in this study may be useful for the further examination of tumorigenesis of large granular lymphoma in the cat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01311052
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  • 10
    Electronic Resource
    Electronic Resource
    Bethanechol and a G-protein activator, NaF/AlCl3, induce secretory response in Paneth cells of mouse intestine (1992)
    Springer
    Cell & tissue research 269 (1992), S. 213-220 
    Details
    ISSN: 1432-0878
    Keywords: Paneth cells ; Ultrastructure ; Morphometry ; Bethanechol ; Fluoride ion ; G-protein ; Mouse (Balb/c)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Paneth cells located at the bottom of intestinal crypts may play a role in controlling the bacterial milieu of the intestine. Using morphometry to clarify the secretory mechanism of the Paneth cells, we studied the ultrastructural changes in mouse Paneth cells produced following intra-arterial perfusion with Hanks' balanced salt solution containing a cholinergic muscarinic secretagogue (bethanechol), a neuroblocking agent (tetrodotoxin), or a G-protein activator (NAF/AlCl3). Bethanechol (2×10-4 mol/l) induced Paneth-cell secretion. Many Paneth cells massively exocytosed their secretory material into the crypt lumen; the enhanced secretion caused degranulation and vacuole formation. However, tetrodotoxin (2×10-6 mol/l) did not prevent the bethanechol-enhanced secretion by the Paneth cells. NaF (1×10-2 mol/l) and AlCl3 (1×10-5 mol/l) induced massive exocytosis of the Paneth cells; the exocytotic figures were similar to those observed in mice stimulated by bethanechol. G-protein activation was followed by a sequence of intracellular events, resulting in exocytosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00319611
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