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  • 1
    ISSN: 1432-1424
    Keywords: intestine ; epithelial transport ; chloride channels ; selectivity ; inactivation ; bilayer reconstitution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary Vesicles derived from epithelial cells of the colonic mucosa of the rat were fused to planar phospholipid bilayer membranes, revealing spontaneously switching anion-conducting channels of 50 pS conductance (at-30 mV with 200mm Cl− each side). The equilibrium selectivity series was I− (1.7)/Br− (1.3)/Cl− (1.0)/F− (0.4)/HCO 3 − (0.4)/Na (〈0.11.). Only one dominant open-state conductance could be resolved, which responded linearly to Cl− concentrations up to 600mm. The singlechannel current-voltage curve was weakly rectifying with symmetrical solutions. When 50 mV were exceeded at the highconductance branch of the curve, switching was arrested in the closed state. At more moderate voltages (±40 mV) kinetics were dominated by one open state of about 35-msec lifetime and two closed states of about 2 and 9-msec lifetime. Of these, the more stable closed state occurred less often. At these voltages one additional closed state of significantly longer lifetime (〉0.5 sec) was observed.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 23 (1988), S. 0 
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A double labeling technique was developed to detect T cell phenotype and HLA-DR (activation) antigens on infiltrating cells in gingival biopsy sites classified as either periodontally active (≥ mm attachment loss within past 3 months), clinically similar but stable, or healthy. Serial cryostat sections were obtained from each of the above disease category biopsies in 13 periodontal maintenance patients, and were processed with sequential Leu series (T cell subsets) avidinbiotin-peroxidase followed by anti-HLA-DR indirect alkaline phosphatase labeling protocols. Labeled cells were counted in repeatable fields in the sulcular, middle and oral thirds of each section, and HLA-DR+ T cells (activated) were calculated. Total HLA-DR-labeled cells and HLA-DR-positive pan-T cells and T helper cells (Th) were all more numerous in active specimens than in healthy sites (p〈0.05), and in sulcular fields than in the oral third of the section (p〈0.05). Although activated T suppressor cell (T5) densities did not vary statistically according to tissue location, active biopsies had more cells per field than either stable or healthy specimens (p〈0.05), especially in the sulcular third. A majority of pan-T, Ts and Th cells appeared to display activation markers in active, stable and healthy biopsies. This study supports the functional role of T lymphocytes in modulating the immune response in periodontal tissues.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 329 (1988), S. 507-508 
    ISSN: 1434-601X
    Keywords: 21.10−k ; 27.60.+j
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The low spin structure of128Xe has been studied by means of the (α,n) reaction using beam energies near the Coulomb barrier. An extended level scheme with spins I≤10ħ and many new spin and parity assignments could be established. The results point to the completeness of the reaction at low spins and excitation energies below 2.5 MeV.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 330 (1988), S. 221-222 
    ISSN: 1434-601X
    Keywords: 21.10.Re ; 23.20.Lv ; 27.60.+j
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract High spin states of126Xe have been populated by means of the116Cd(13C, 3n) reaction using the OSIRIS-12 detector system. A level scheme consisting of 13 bands has been established. More than 40 new levels were observed and several spin and parity assignments could be made. Experimental Routhians and alignments of selected bands are presented.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 323 (1986), S. 487-488 
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Excited states of126Ba have been investigated via the Sn(13C, 3n)-reaction by means of inbeam gamma ray spectroscopy using compton suppressed germanium detectors of the OSIRIS facility. 37 new levels were observed and spin and parity assignments could be made. Besides the ground and the quasi-gamma band two positive parity superbands and at least 4 more sidebands of presumably negative parity were established.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 327 (1987), S. 251-265 
    ISSN: 1434-601X
    Keywords: 21.10−k ; 21.10.Hw ; 27.60.+j
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract High spin states of126Ba have been populated via the116Sn (13C, 3n) reaction and were investigated by inbeam gamma-ray spectroscopy. Partially NaJ (Tl)-BGO anti-compton shields were used. Two positive parity band crossings were established. The yrast band was observed up to a tentative 18+ level. The gamma-bands and two band structures of negative parity were found. In addition a tentative 3− state has been seen. Two-quasiparticle Routhians are constructed from the one-quasiparticle Routhians of the bands in the odd neighbouring isotopes and isotones and are compared to the ones observed in126Ba. This leads to ah 11/2 g 7/2-neutron structure of the strongly coupled negative parity bands and a two-quasiproton structure of the decoupled bands of negative parity. The branching and mixing ratios for the neutron bands are analyzed.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 328 (1987), S. 15-18 
    ISSN: 1434-601X
    Keywords: 21.10.Re ; 23.20.Lv ; 27.60.+ j
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract High spin states of128Ba have been investigated by means of the OSIRISγ-spectrometer with 6 asymmetric anti-compton shields and one additional germanium detector. The110Pd(22Ne, 4n) reaction was used at 96 MeV. Several new high spin states and a new band have been established. The onset of the ph 11 2/2 nh 11 2/2 4 quasiparticle band has possibly been found.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 331 (1988), S. 113-114 
    ISSN: 1434-601X
    Keywords: 21.10.−k ; 23.20.Lv ; 27.60,+j
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Excited states of129Xe have been investigated through the126Te(α,n)129Xe reaction at the incident energy E=16MeV by means of in-beam gamma ray spectroscopy. Numerous levels below the excitation energy of 2.5MeV were found. Spin assignments were made from side feeding excitation functions. A new level at 274.2keV, which is suggested as spin 9/2 negtive parity bandhead, was observed for the first time.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Plasma chemistry and plasma processing 5 (1985), S. 293-316 
    ISSN: 1572-8986
    Keywords: Plasma chemistry ; halocarbon decomposition ; kinetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Technology
    Notes: Abstract For the case of weak feed gas decomposition, where the concentration of CCl4 exceeds those of decomposition and built-up products, the emission of CCl* is shown to originate from dissociative excitation of CCl4. With electron concentration measured independently, the kinetics of CCl4 decomposition has been extracted from the time dependence of the CCl* intensity. Supported by EPR determinations of radical concentrations in rapidly flowing CCl4 and CCl4/O2 afterglows, the primary decomposition reaction is shown to be the electron impact dissociation into CCl3 and Cl. Its rate constant (k 1=4×10−8 cm3s−1) indicates strongly that dissociative electron attachment is the main reaction channel at least at r.f. power densities just above the threshold of a self-maintaining discharge. At extremely low mean electron energies the emission of a continuum is observed, which is tentatively ascribed to the radiative CCl3-Cl recombination.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1612-1112
    Keywords: Discontinuous reversed-phase chromatography ; 50S subunit ribosomal proteins ; Sulfolobus acidocaldarius ; Archaebacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The total protein of the 50S ribosomal subunit (TP50) from the archaebacterium Sulfolobus acidocaldarius was prefractionated by discontinuous reversed-phase HPLC with several column combinations. The purity of the eluted fractions was tested by sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) or two-dimensional PAGE (2D-PAGE). With regard to the load capacity and selectivity, best results were obtained with a semi-preparative three-column combination (total length 67.5cm). Twelve A230 units of TP50 were separated into 35 fractions, 19 of which contained nearly pure proteins. When the sample load was increased to 176A230 units (109mg), 13 of 28 collected fractions still contained nearly pure proteins. The selectivity of a semi-preparative short-column combination (total length 12cm) was similar to that of the semi-preparative column combination, and separation time could be reduced to one third of that required for the longer column combination. The load capacity of the short-column combination was lower than that of the semi-preparative column combination.
    Type of Medium: Electronic Resource
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