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Digitale Medien

An immunofluorescence study of influenza virus filament formation (1980)

Cox, J. C. ; Hampson, A. W. ; Hamilton, R. C.

Springer

Archives of virology 63 (1980), S. 275-284

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ISSN: 1432-8798

Quelle: Springer Online Journal Archives 1860-2000

Thema: Medizin

Notizen: Summary A study is described in which filamentous forms of influenza virus were observed budding from host cell surfaces. Cell cultures infected with influenza virus were stained by indirect immunofluorescence using an antiserum to purified haemagglutinin. Filaments greater than 100 µm in length, with several branch points along their length were observed; the number and length of the filaments varied according to the virus strain and the time after infection. Examination of infected cells by electron microscopy confirmed the presence of branched structures with an ultrastructure typical of filamentous forms of influenza virus. The immunofluorescence technique was quicker than thin section electron microscopy and was a more sensitive procedure for the detection of filamentous forms of influenza virus than electron microscopy using negative stain. It also enabled the antigenic composition of the filaments to be observed.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF01315033

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Digitale Medien

The ultrastructure ofEncephalitozoon cuniculi growing in renal tubules of rabbits (1981)

Hamilton, R. C. ; Cox, J. C.

Springer

Parasitology research 64 (1981), S. 271-278

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ISSN: 1432-1955

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Abstract A wild type rabbit was infected orally with cell culture-grownEncephalitozoon cuniculi. Twelve weeks after infection the rabbit was killed and blocks of kidney tissue were fixed for histology and electron microscopy.E. cuniculi were observed within kidney collecting tubule cells. The ultrastructure and development ofE. cuniculi in these cells was similar to that described in cultured cells and peritoneal macrophages.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00927374

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Digitale Medien

High level production of potyvirus-like particles in insect cells infected with recombinant baculovirus (1994)

Edwards, S. J. ; Hayden, M. B. ; Hamilton, R. C. ; [weitere]

Springer

Archives of virology 136 (1994), S. 375-380

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ISSN: 1432-8798

Quelle: Springer Online Journal Archives 1860-2000

Thema: Medizin

Notizen: Summary The full length gene for the coat protein (CP) of the potyvirus, Johnsongrass mosaic virus, was incorporated into recombinant baculovirus and expressed in insect cells. Western blot and Coomassie-stained polyacrylamide gel electrophoresis analysis of infected insect cells demonstrated that CP was produced in large quantity. Electron microscopic examination of these cells showed the presence of numerous potyvirus-like particles in the cytoplasm. Morphologically the particles resembled potyvirus particles assembled in vitro in the absence of viral RNA and those found inEscherichia coli expressing the recombinant CP gene.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF01321065

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