ISSN:
1432-8798
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The host range of the bovine RS virus, strain NMK7, recovered from a diseased cow during the 1968/69 outbreak in Japan was examined both in experimental animals and cell cultures. The virus strain could be propagated serially in the brain of one-day-old DDY mice, although the virus yield was low and no illness was produced. On the other hand, the virus failed to infect adult DDY mice by various routes of inoculation. Young guinea pigs inoculated with virus intracerebrally, intranasally, intratracheally or intraperitoneally developed no illness but produced low titers of specific neutralizing antibody, indicating the occurrence of an inapparent infection. In tissue cultures, the bovine RS virus showed a wide range of susceptible host cells. It replicated in all cell species (kidney, testicle, thyroid, thymus, duodenum, rectum) of bovine origin as well as in cell cultures from swine (embryonic kidney), hamster (lung, kidney), monkey kidney (Vero), and human (embryonic lung and kidney, HeLa, HEp-2). Better growth was observed in bovine than in human cell cultures. The simultaneously tested human RS virus (strain Long) exhibited a smaller host cell range in cultures of animal provenance and showed optimal growth in human cells. Both virus strains failed to propagate in chicken cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01240616
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