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  • 1965-1969  (62)
  • Biochemistry and Biotechnology  (51)
  • Physical Chemistry  (11)
  • 1
    Digitale Medien
    Digitale Medien
    Large-scale isolation of prolyl-tRNA synthetase from Phaseolus aureus (1967)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 9 (1967), S. 343-356 
    Details
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Some of the problems involved in the scale-up of enzyme isolation procedures have been examined. In particular the isolation of prolyl-tRNA synthetase has been investigated. Using a modified procedure (500 × laboratory scale) the enzyme has been separated from 25 kg. of Phaseolus aureus (mung bean) and Partially purified by acid precipitation followed by fractional precipitation with ammonium sulfate. The yield of enzyme was 42 g. (10.5% recovery).
    Zusätzliches Material: 2 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bit.260090307
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  • 2
    Digitale Medien
    Digitale Medien
    Studies with dried airborne vaccinia virus in a small environmental chamber (1968)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 10 (1968), S. 151-157 
    Details
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Dried vaccinia virus was found to be very stable in aerosol form in our chamber at a temperature of 75°F and a relative humidity (RH) of 85% when the virus was protected with 1.5% lysine, 1.5% sodium glutamate, 0.5% isoniazid, 0.5% thiourea, and either 2.5% heart infusion broth (HIB) or a combination of 3.75% lactose plus 1.25% raffinose prior to freeze-drying. Utilization of the Environmental chamber technique resulted in (a) selection of two diluents which protected vaccinia virus against the effects of high relative humidity, (b) measurement of the effect of various chemical additives in the diluents, and (c) quantitative measurement of the moisture absorbed by the various dried products.
    Zusätzliches Material: 2 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bit.260100204
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  • 3
    Digitale Medien
    Digitale Medien
    In vitro synthesis of polyribonucleotides (1968)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 10 (1968), S. 359-372 
    Details
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Polyribonucleotide: orthophosphate nucleotidyl transferase, commonly known as polynucleotide phosphorylase catalyzes the reversible polymerization of ribonucleoside diphosphates with the liberation of orthophosphate. The equilibrium constant is approximately 1.0. Although isolated from a variety of sources, the enzyme occurs essentially as two types: one which does not require a primer for reaction initiation and a second which does. A parallel study of an E. coli preparation representing the first type and an M. lysodeikticus preparation representing the second showed differences other than the primer requirement. Rates of polymerization were different as were the Kms. The E. coli preparation catalyzed the synthesis of polyguanylic acid while the M. lysodeikticus preparation did not although synthesis of hetoropolymers containing guanylic acid was catalyzed by the M. lysodeikticus enzyme. Use of repurified commercial substrates made the validity of some primer-requirement experiments suspect. End group analysis of product polymers served only to raise questions concerning the reaction-initiating compounds and the reaction mechanism. A study of hetero-polymer synthesis showed not only that the rate of polymerization was different, from that of homopolymers but that uncompetitive inhibition rather than competitive inhibition occurred when two ribonucleoside diphosphates were present in the reaction mixture. Furthermore, the experiments showed a preferential uptake of one substrate over another and an “enrichment” which was constant. It has also been shown that RNA polymerase, a DNA-RNA directed polymerase, can be used to synthesize polyribonucleotides if the appropriate template is provided.
    Zusätzliches Material: 8 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bit.260100309
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  • 4
    Digitale Medien
    Digitale Medien
    Large-Scale isolation of acid phosphatase from human erythrocytes (1968)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 10 (1968), S. 829-843 
    Details
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: A laboratory process for the isolation of type B acid phosphatase from human erythrocytes has been scaled up 80 times. The enzyme was purified 1300-fold by adsorption on calcium phosphate gel, ammonium sulfate precipitation, and gel filtration. The yield was 27%. Results for the type A enzyme are also presented.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bit.260100609
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  • 5
    Digitale Medien
    Digitale Medien
    Kinetics of product inhibition in alcohol fermentation (1968)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 10 (1968), S. 845-864 
    Details
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: The inhibitory effect of ethanol concentration p in a medium on the specific rates of growth μ and ethanol production ν of a specific strain of baker's yeast was studied in a chemostat, where except for ethanol as the product, only the concentration of glucose S was controlled to limit the metabolic activity of the yeast. This was designed to supplement the previous findings from the batch experiment, in which ethanol was added artificially and no substrate components were limiting the metabolism of the same yeast, that μ = μ0e-k1p and ν = ν0e-k2p, where k1 and k2 are empirical constants and subscript the 0 denotes respective values at p = 0. The effects of p on the values of μ and ν were confirmed by the Lineweaver-Burk plot to belong to noncompetitive inhibition. The formulas here for μ and ν as affected by p, if extrapolated to the case of no limiting substrates, were in good agreement in respective forms with those derived previously from the batch experiment, though the values of corresponding coefficients in these formulas were different. The differential equations for μ and ν as functions of both p and S and, in addition for the rate of glucose consumption as correlated by the yield factors either with the cell growth rate or the rate of ethanol production, were solved properly with a digital computer. A kinetic, pattern calculated so far was discussed with reference to the data obtained in the batch experiment and those relevant to actual “sake” brewing.
    Zusätzliches Material: 11 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bit.260100610
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  • 6
    Digitale Medien
    Digitale Medien
    The isolation of an aliphatic amidase from Pseudomonas aeruginosa (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 283-292 
    Details
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: A pilot-scale process for the isolation of an aliphatic, amidase from Pseudomonas aeruginosa has been developed. A constitutive, partially irrepressible mutant was employed to give a high initial enzyme concentration. An existing laboratory isolation procedure has been scaled up and modified particularly by substitution of polyethylene glycol for ammonium sulfate precipitation as the first stage in the conversion of the fractionation to continuous operation. Full recovery of activity was achieved with the modification. The recovery of enzyme from a subsequent chromatographic stage was 85% and the maximum overall purification was 28-fold.
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bit.260110303
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  • 7
    Digitale Medien
    Digitale Medien
    Growth of mouse fibroblast-like cells in an autoclavable medium (1968)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 10 (1968), S. 61-67 
    Details
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: A relatively simple, autoclavable medium was developed that would support the growth of the mouse fibroblast (MFL) cell line in suspension culture. This medium was prepared in three stages with decreasing quantities of serum. As the serum was reduced from 5% to none, the amount of Bacto-Peptone was increased from none to 0.5%. The reduction and finally elimination of serum did not affect proliferation adversely, but actually seemed to produce more rapid growth and higher levels of cell population.
    Zusätzliches Material: 3 Tab.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bit.260100105
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  • 8
    Digitale Medien
    Digitale Medien
    Kinetics of product inhibition in alcohol fermentation-temperature effect in the “sake” brewing (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 1285-1287 
    Details
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: One of the kinteic equations derived previously from a series of sophisticated batch and continuous alcohol fermentations by using a respiration-deficient mutant of baker's yeast is as follows: \documentclass{article}\pagestyle{empty}\begin{document}$$ {{dp} \mathord{\left/ {\vphantom {{dp} {dt}}} \right. \kern-\nulldelimiterspace} {dt}} = v_0 e^{ - k_2 p} \left[{{S \mathord{\left/ {\vphantom {S {\left({K_s ^\prime + S} \right)}}} \right. \kern-\nulldelimiterspace} {\left({K_s ^\prime + S} \right)}}} \right]X $$\end{document} where dp/dt = ethanol production rate, v0 = specific rate of ethanol production at p = 0, k2 = empirical constant, K′s = saturation constant, S = glucose concentration, and X = cell mass concentration. The above equation was confirmed in the previous paper to fit, the brewing of “sake.”The temperature of the specific brewing is not always constant (10 to 18°C). The effect of temperature on v0 was assessed from the Arrhenius plot, assuming that k2 was independent of temperature. Values of dp/dt taken from the “sake” brewing data were rearranged, taking the temperature change into account. These datu, corrected for the temperature, were found to follow quite favorably the kinetic equation mentioned above. So far, a prediction of the ethanol production rate in practice was rectified to the extent of p = 19%.
    Zusätzliches Material: 1 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bit.260110621
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  • 9
    Digitale Medien
    Digitale Medien
    Studies of cage reactions III. A test over a wide temperature range (1969)
    New York, NY : Wiley-Blackwell
    International Journal of Chemical Kinetics 1 (1969), S. 357-359 
    Details
    ISSN: 0538-8066
    Schlagwort(e): Chemistry ; Physical Chemistry
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Zusätzliches Material: 1 Tab.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/kin.550010308
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  • 10
    Digitale Medien
    Digitale Medien
    Antimicrobial agents as liquid chemosterilizers (1965)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 435-443 
    Details
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Many antimicrobial agents are available for commercial use, but only a few are truly sporicidal and can be used as chemosterilizers. The action of ethylene oxide, propylene oxide, and β-propiolactone is well documented. Strong acids, e.g., hydrochloric acid, or alkalis may be used as chemosterilizers but their use is extremely limited. The synthetic phenolics accompanied by boiling were recommended, but heat cannot always be employed. Chlorine and iodine have been used in higher concentrations. The use of 8% formaldehyde or 20% formalin was suggested by Spaulding.17 Other aldehydes possess antimicrobial potency. According to work performed in our laboratories, the sporicidal activity of certain alcoholic, saturated dialdehyde solutions equalled or surpassed that of formaldehyde. These included glyoxal, succinaldehyde, and glutaraldehyde. Aqueous alkaline glutaraldehyde solutions killed the most resistant bacterial spores, e.g., B. subtilis and Cl. tetani, within a few hours (Borick1,2). Glutaraldehyde, a 5-carbon dialdehyde, was shown to be a chemosterilizer when tested by various methods, destroying bacteria, including M. tuberculosis, fungi, and viruses. Acid glutaraldehyde solutions stored at room temperature are highly stable, whereas alkaline solutions show a significant change in pH and a diminution of glutaraldehyde concentration on alkalinization. For this reason, fresh (not over 2 weeks old) alkaline glutaraldehyde solutions can be used as chemosterilizers.
    Zusätzliches Material: 1 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bit.260070310
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