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  • *Membrane transport  (1)
  • 07.60. Hv  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 30 (1996), S. 107-114 
    ISSN: 1432-0983
    Keywords: Key words  Yeast ; Glutamine permease ; *Membrane transport ; Nitrogen-regulated gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   Glutamine uptake in S. cerevisiae is mediated by at least three transporters: high- and low-affinity glutamine permeases and the general amino-acid permease. We have isolated the gene encoding the high-affinity glutamine permease and named it GNP1. The amino-acid sequence of GNP1, and its hydropathy profile of 12 transmembrane domains, closely resemble those of known amino-acid permeases. The Km of GNP1 for glutamine uptake was determined to be 0.59 mM. Cells lacking GNP1 exhibit reduced levels of glutamine transport, and are resistant to a toxic analog of glutamine, L-glutamic acid γ-monohydroxamate. Unlike other amino-acid permeases, whose expression is nitrogen-source limited, GNP1 is expressed on both rich and poor nitrogen sources.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Applied physics 52 (1991), S. 317-322 
    ISSN: 1432-0630
    Keywords: 78.65. -s ; 07.60. Hv ; 68.35. Fx
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract We report the detection of linear optical diffraction of a He-Ne laser probe beam from a monolayer grating of molecular adsorbates up to the fifth order with no electronic or electromagnetic enhancement. The advantages of using linear diffraction to probe surface diffusion of adsorbates are discussed.
    Type of Medium: Electronic Resource
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