ISSN:
1432-069X
Keywords:
2,4-Dinitrochlorobenzene
;
2,4-Dinitrophenylated epidermal cells
;
Contact sensitivity
;
Spontaneous flare
;
Delayed-type skin reaction
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The percutaneous administration of in vitro haptenated epidermal cells (EC) has become established as a procedure to produce contact sensitivity (CS) in experimental animals for routine use. The cells have also been found to elicit a significant delayed-type skin reaction by intradermal test in the animals sensitized by painting the skin with the hapten. The fate of 2,4-dinitrophenylated (DNP) isogeneic epidermal cell suspensions (EC) injected intradermally was investigated histologically in intact or 2,4-dinitrochlorobenzene (DNCB)-sensitized strain 13 guinea pigs to study the role of the cells in CS. DNP-EC were found to proliferate actively in the dermis and formed EC nests with central keratinization and then elicited inflammatory reaction associated with necrosis of the epidermal structures 7 days after injection in the intact animals. DNP-EC injected intradermally into the animals which had received and reacted against DNCB underwent a suppression of EC proliferation. These findings are discussed in relation to the role of the haptenated EC in CS.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00585933
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