Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Modulation of 5-fluorouracil with methotrexate and low-dose N-(phosphon-acetyl)-L-aspartate (PALA) is inactive in advanced pancreatic carcinoma (1997)
    Springer
    Annals of oncology 8 (1997), S. 917-918 
    Details
    ISSN: 1569-8041
    Keywords: biochemical modulation ; 5-fluorouracil ; methotrexate ; pancreatic cancer ; phosphonacetyl-L-aspartate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Purpose: To evaluate the effect of biochemical modulation by PALA and methotrexate on the therapeutic activity of 5-fluorouracil (5-FU) in patients with advanced pancreatic adenocarcinoma. Patients and methods: The treatment protocol consisted of phosphonacetyl-L-aspartate (PALA) 250 mg/m2 i.v. 15-minute infusion followed by methotrexate 200 mg/m2 i.v. 30-minute infusion on day 1 and 5-FU 600 mg/m2 i.v. push on day 2. Folinic acid was given at 15 mg/m2 p.o. every six hours for eight doses, starting 24 hours after methotrexate infusion. Cycles were repeated every two weeks. Results: Thirty patients with advanced chemotherapy-naive pancreatic cancer were included; 26 had measurable disease. Median age 56 years (27–72); median PS 1 (0–2). One PR (3.9%) was achieved; nine patients had stable disease. Median time to progression was 91 days. Median survival was 177 days and one year survival was 13.3% (4 of 30 patients). Treatment was well tolerated; diarrhea WHO grade 2 or 3 occurred in six patients; stomatitis WHO grade 2 and 3 in nine patients. Conclusions: Modulation of 5-FU by PALA and MTX given in this dose and schedule appears to be ineffective in patients with advanced pancreatic adenocarcinoma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008298102758
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Isolation of rapidly labelled nuclear RNA of high specific activity from human leukaemic cells (1973)
    Springer
    Journal of molecular medicine 51 (1973), S. 677-679 
    Details
    ISSN: 1432-1440
    Keywords: Human Leukaemia ; 32P-orthophosphate ; nuclear RNA ; Menschliche Leukämie ; 32P-Orthophosphat ; nucleare RNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Es werden Methoden zur Präparation von nuklearer RNA hoher spezifischer Aktivität aus menschlichen Leukämiezellen beschrieben. Das Inkubationsmedium basiert auf Hepes-Puffer und der Verwendung von dialysiertem Kalbsserum zur Verbesserung der Bedingungen für den32P-Orthophosphat-Einbau in die hochmolekulare Kern-RNA. Die erreichten spezifischen Aktivitäten erlauben detaillierte Nucleotid- und Oligonucleotidanalysen der verschiedenartigen Ribonucleinsäurespezies in menschlichen Leukämiezellen.
    Notes: Summary Methods are presented which provide the preparation of highly labelled nuclear RNA from cells of the different forms of human leukaemia. An incubation medium is described that is based on Hepes buffer and on the use of exhaustively dialyzed fetal calf serum offering suitable conditions for the uptake of32P-orthophosphate into the RNA of leukaemic nuclei. The specific activities reached may allow more detailed nucleotide and oligonucleotide analyses of the various RNA species present in human leukaemic cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01468172
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Differences in nuclear RNA labelling patterns of BURKITT lymphoma, leukaemic lymphosarcoma and various human leukaemias (1973)
    Springer
    Journal of molecular medicine 51 (1973), S. 680-684 
    Details
    ISSN: 1432-1440
    Keywords: BURKITT lymphoma ; leukaemic lymphosarcoma ; chronic myelotic leukaemia (CML) ; acute myeloblastic leukaemia (AML) ; chronic lymphocytic leukaemia (CLL) ; 32P-orthophosphate ; nuclear RNA ; Burkitt-Lymphom ; leukämische Lymphosarcomatose ; akute myeloische Leukämie (AML) ; chronischmyeloische Leukämie (CML) ; chronisch-lymphatische Leukämie (CLL) ; 32P-Orthophosphat ; nucleare RNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Aus Zellen des Burkitt-Lymphoms, eukämischer Lymphosarcomatose, chronisch-lymphatischer Leukämie, chronisch-myeloischer und akuter myeloischer Leukämie wurden nach sechsstündiger32P-Markierung in einem phosphatarmen Medium Zellkerne mit Hilfe des Zitronensäure-Verfahrens isoliert und die nucleare RNA mit der heißen Phenol-SDS-Methode extrahiert. Nach Fraktionierung der kernspezifischen Nucleinsäuren über Zucker-Dichtegradienten fanden sich markante Unterschiede in der32P-Radioaktivitätsverteilung. Insbesondere war eine differente Markierung der nuclearen 45S RNA, welche als Vorläufer ribosomaler 28S und 18S RNA gilt, festzustellen. Die niedrigsten spezifischen Aktivitäten des ribosomalen Vorläufers fanden sich bei der CML, die höchsten bei AML, leukämischem Lymphosarkom und Burkitt-Tumor. Bemerkenswert erscheint die aktive Synthese präribosomaler (45S/35S) RNA in Zellen der CLL, deren DNA-Syntheserate äußerst niedrig ist. Zur Klärung der Frage, ob die im Nucleolus der unreifen Zellen gebildeten hochmolekularen Nucleinsäuren quantitative oder qualitative Unterschiede zwischen myeloischen und lymphatischen Zellen aufweisen, sind strukturchemische Untersuchungen im Gange.
    Notes: Summary Nuclear RNA was isolated from citric acid nuclei derived from AML, CML, CLL, leukaemic lymphosarcoma and BURKITT lymphoma cells after 6 hours incubation with32P-orthophosphate in a phosphate-free medium. In fractionations on sucrose density gradients, marked differences were found in the distribution of the32P-radioactivity mainly in the 45S fraction containing the ribosomal precursor RNA. The lowest specific activities of nuclear 45S RNA were found in CML; very high labelling accurred in cells of AML, leukaemic lymphosarcoma and BURKITT lymphoma. In CLL cells which are known for lack in DNA synthesis, pre-ribosomal 45S and 35S RNA were labelled to a remarkable extent. Studies are in progress in order to define possible differences in nuclear RNA structures between lymphocytic and granulocytic cell lines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01468173
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...