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  • 1
    ISSN: 1432-1440
    Keywords: Hyperthyroidism ; Erythrocytes ; Na-K ATPase ; Na-Li countertransport ; Intracellular sodium concentration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary To investigate the effect of thyroid hormones on erythrocyte cation transport systems and intracellular electrolyte content we have measured the activity of Na-K ATPase, Na-Li countertransport, as well as red cell sodium and potassium contents in patients with hyperthyroidism and in euthyroid controls. Intracellular Na- and K-concentrations were determined in erythrocytes washed three times in isotonic MgCl2 solution. Ouabain-sensitive Na-transport was estimated as the increase of Na before and after addition of ouabain in an erythrocyte suspension in isotonic Na-free medium. Na-Li countertransport was measured according to the method described by Canessa et al. [2]. The patients with hyperthyroidism exhibited a significantly elevated intracellular sodium content as well as a highly increased Na-K ATPase activity. Intracellular potassium content was not altered in the hyperthyroid subjects, but Na-Li countertransport was markedly decreased as compared to the controls. The results indicate that different ion transport systems of the erythrocyte membrane are influenced by thyroid hormones. We suggest that the elevation of Na-K ATPase activity might be due to the increased intracellular sodium concentration which is caused by the diminished countertransport pathway. Furthermore, the activity of Na-K ATPase, Na-Li countertransport, and intracellular sodium content in erythrocytes might be a useful peripheral indicator of thyroid hormone excess.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Annals of hematology 39 (1979), S. 141-145 
    ISSN: 1432-0584
    Keywords: Alkalische Leukozytenphosphatase (ALP) ; maligne Erkrankungen ; Metastasen ; Leukocyte alkaline phosphatase (LAP) ; Malignant disease ; Metastases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The leukocyte alkaline phosphatase (LAP) levels were determined in 183 patients with malignant diseases and 71 normal controls. The median LAP scores were 64 units (range 0 to 290) for the patients and 55 (range 2 to 158) for the controls, respectively, and no significant difference could be established. When analyzed according to primary malignancy, only in patients with Hodgkin's disease (n = 14) was the median value higher than normal (p 〈 0.001). In patients with distant metastases (n = 48), higher LAP levels were demonstrated (M = 76, range 21 to 290) as compared to patients with no evidence of metastases (M = 53, range 0 to 229), (p 〈0.01). Thus, LAP activity has very limited value in the diagnosis of malignancies. Its elevation in the presence of malignant disease might, however, indicate metastases.
    Notes: Zusammenfassung Bei 183 Patienten mit malignen Erkrankungen und 71 Kontrollpersonen wurde der ALP-Index untersucht. Der Median für die Patienten war 64 (range 0–290) und für die Kontrolle 55 (range 2–158). Dieser Unterschied ist jedoch statistisch nicht signifikant. Bei der Analyse der ALP-Indices nach Tumorgruppen konnte eine signifikante Erhöhung des ALP-Index nur bei Morbus Hodgkin (n = 14) festgestellt werden (p 〈0,001). Der ALP-Index war auch bei Patienten mit Metastasen (n = 48) (M = 76, range 21 zu 290) im Vergleich zum ALP-Index bei Patienten ohne Metastasen (n = 118, M = 53 range 0–229) erhöht (p 〈0,01). Die ALP-Aktivität hat also nur einen beschränkten Wert in der Diagnostik bei malignen Erkrankungen. Eine Erhöhung kann jedoch auf das Vorliegen von Metastasen hinweisen.
    Type of Medium: Electronic Resource
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