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  • Analytical Chemistry and Spectroscopy  (11)
  • Brassica oleracea  (5)
  • 1
    Electronic Resource
    Electronic Resource
    Screening of Portuguese cole landraces (Brassica oleracea L.) with Peronospora parasitica and Plasmodiophora brassicae (1993)
    Springer
    Euphytica 67 (1993), S. 135-141 
    Details
    ISSN: 1573-5060
    Keywords: downy mildew ; clubroot ; disease resistance ; Tronchuda cabbage ; Galega kale ; Brassica oleracea ; Peronospora parasitica ; Plasmodiophora brassicae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Forty-four landraces of Portuguese coles (Brassica oleracea L.), representing different morphotypes, were screened at the cotyledon stage for downy mildew resistance, caused by Peronospora parasitica. Two isolates of the pathogen, PHW 630 and PHW 828, were used in the experiment. The mean disease interaction phenotype varied among the landraces. Overall, the Portuguese coles have shown resistance to both isolates, particularly to isolate PHW 828. Some landraces were identified with differential reaction to the two P. parasitica isolates. Some of the Portuguese coles are, therefore, good sources of resistance genes to P. parasitica, and could be used in breeding programs or in the development of a downy mildew host differential set. Among the morphotypes tested, Couve Algarvia (ISA 207), Couve Galega (ISA 243 and 187) and Couve Glória de Portugal (ISA 84) have presented the highest resistance interaction. For the development of host differential sets, the landraces ISA 2 (Penca de Mirandela), ISA 35 (Couve de Valhascos) and ISA 600 (Penca Espanhola) could be further selected for the differentiation of P. parasitica isolates. They are resistant to PHW 828 and susceptible to PHW 643. The same morphotypes and landraces were also screened at the seedling stage for resistance to clubroot, caused by Plasmodiophora brassicae Woron.. Although some variation for resistance could be noticed in some the landraces tested, mainly Galega kale types, none of them presented a sufficient level of resistance as to be directly be useful in breeding for resistance to race 6 of P. brassicae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00022736
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  • 2
    Electronic Resource
    Electronic Resource
    Genetic diversity and taxonomy of Portuguese Tronchuda cabbage and Galega kale landraces using isozyme analysis (1994)
    Springer
    Euphytica 75 (1994), S. 221-230 
    Details
    ISSN: 1573-5060
    Keywords: allozyme variation ; allozyme polymorphism ; Brassica oleracea ; classification ; Portuguese coles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Isozyme analysis was used to study the genetic variation and the genetic relationships of a collection of 48 Brassica spp. including 31 Portuguese coles (Brassica oleracea L.) accessions representative of the different landraces cultivated in Portugal. Other brassicas included in this experiment were Jersey kale, kailaan, common cabbages, broccolis, cauliflower, nine-chromosome wild brassica and turnip. Nine enzymes used in the starch gel electrophoresis included: PGM, PGI, AAT, LAP, TPI, FBP, SOD, IDH and GR. Twenty-one putative loci were revealed, with 3 showing invariance and the other 18 contained 50 alleles. The allelic frequencies at these loci represented by 40 plants per accession were used to calculate the following estimators of genetic variation: % of polymorphic loci, average number of alleles per polymorphic loci, average number of alleles per locus, and index of heterozygosity. The genetic relationships were evaluated considering Nei (1978) and Rogers (1972) genetic distances between each pair of accessions whose matrices were hierarchically clustered by the UPGMA method. The accessions were also studied using the principal coordinate analysis. Portuguese Tronchuda cabbages and Galega kales have shown high genetic diversity in comparison with the other accessions. This indicates their potential variation for use in breeding programs. The UPGMA results show that the 48 accessions, with the exception of B. insularis, B. cretica, and turnip, can be clustered into 6 groups: (a) Portuguese Tronchuda cabbages, Galega kales and Algarve cabbages; (b) common cabbages and kales; (c) Couve Poda do Algarve and broccoli; (d) Algarve cabbage and common cabbages; (e) kailaan; (f) broccoli and cauliflower. The groupings obtained by the isozyme analysis are difficult to interpret considering the origin of the Brassica spp. and the morphological resemblance among the accessions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00025607
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  • 3
    Electronic Resource
    Electronic Resource
    Screening of Portuguese cole landraces (Brassica oleracea L.) with Leptosphaeria maculans and Xanthomonas campestris pv. campestris (1992)
    Springer
    Euphytica 65 (1992), S. 219-227 
    Details
    ISSN: 1573-5060
    Keywords: Phoma lingam ; Leptosphaeria maculans ; Xanthomonas campestris pv campestris ; black leg ; black rot ; disease resistance ; Brassica oleracea ; Portuguese coles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Fifty-six landraces of Portuguese coles (Brassica oleracea l.), representing fourteen morphotypes, were screened for resistance to Leptosphaeria maculans at the cotyledon stage. Three isolates of the pathogen belonging to three pathogenicity groups were used in the screening. The mean disease interaction phenotype of the B. oleracea landraces with the L. maculans isolates confirmed their differences in pathogenicity. When compared to pathogenicity grouping using a B. napus differential set, a different classification was observed on B. oleracea for the three L. maculans isolates, suggesting possible differences in the genetic interaction between the two plant species and the pathogen. Most of the Portuguese cole landraces were found to be susceptible to all three isolates of L. maculans, although a few have shown intermediate interaction phenotype. Some accessions, on the other hand, elicit a differential reaction with the three isolates tested, and can be further selected to be used in host differential sets or in breeding for resistance to black leg. Fifty-four landraces of Portuguese coles, representing eleven morphotypes, were also screened in the field for resistance to Xanthomonas campestris pv. campestris. A good level of resistance to the bacterium was found in several of the morphotypes. Accessions of the ‘Penca’ type, particularly, were identified as highly resistant. Landraces belonging to the same Portuguese cole group presented a similar range of mean interaction phenotype with the bacterium. Some Portuguese landraces of B. oleracea are potential sources for use in breeding programs for black rot resistance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023086
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  • 4
    Electronic Resource
    Electronic Resource
    Molecular taxonomy of Portuguese tronchuda cabbage and kale landraces using nuclear RFLPs (1991)
    Springer
    Euphytica 58 (1991), S. 221-229 
    Details
    ISSN: 1573-5060
    Keywords: Brassica oleracea ; landrace group ; molecular markers ; numerical taxonomy ; Portuguese coles ; RFLPs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary RFLPs were used to study the genetic relationships of 31 Portuguese coles (Brassica oleracea L.) representing the different ecotypes or landraces grown in Portugal. Other cole crops such as Jersey kale, Chinese kales, common cabbages, broccolis and cauliflower, two nine-chromosome wild species, and one accession of turnip also were included to determine the evolutionary relationships of Portuguese coles to other related coles. Principal coordinates and cluster analyses were conducted using a package of computer programs and RFLP data from 55 nuclear DNA probes, detecting 291 polymorphic restriction fragments. The results showed that the 48 accessions clustered into five groups: (a) a dispersed group with turnip and the wild brassicas; (b) Chinese kales; (c) broccolis and cauliflower; (d) a disperse group including Algarve and Jersey kales, thousand head kale, cabbages, and savoy cabbage; (e) a large and compact group containing all Portuguese tronchuda cabbages and kales. This unique and closely related group containing Portuguese tronchuda cabbages and kales could be further divided into five subgroups corresponding to the major areas of cultivation. The phenetic groupings of Portuguese tronchuda cabbages and kales based on RFLP data corresponded more with their geographic origins (collecting sites) than with their morphological similarities. A mechanism involving geographic isolation and frequent intercrossing within local areas is proposed to explain genetic relationships among Portuguese tronchuda cabbages and kales.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00025253
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  • 5
    Electronic Resource
    Electronic Resource
    Numerical taxonomy of Portuguese Tronchuda cabbage and Galega kale landraces using morphological characters (1993)
    Springer
    Euphytica 69 (1993), S. 51-68 
    Details
    ISSN: 1573-5060
    Keywords: Brassica oleracea ; classification ; landrace groups ; morphology ; Portuguese coles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A morphological study was carried out to determine the relationships among Portuguese cole landraces using 58 accessions belonging to: (i) Portuguese Tronchuda cabbage (Brassica oleracea var. tronchuda Bailey syn. var. costata De Candolle, Couve Tronchuda); (ii) Portuguese Galega kale (B. oleracea var. acephala De Candolle, Couve Galega); and (iii) other economically less important coles such as Algarve cabbages (B. oleracea var. capitata L., Couve do Algarve or B. oleracea var. sabauda L., Couve Repolho-lombarda do Algarve). The cole accessions were collected from the growers, studied under field conditions during two consecutive years, and characterized using 46 morphological characters from seedling stage to ripe silique. Morphological data were analysed by numerical taxonomy techniques using UPGMA (Unweighted Pair Group Method Using Arithmetic Averages) and by principal component analysis (PCA). Phenograms based on correlation and distance coefficients showed the existence of 8 main groups (A-H). Five of these groups correspond to Tronchuda cabbage landraces: (A) “Couves de Trás-os-Montes”; (B) “Couves do Minho”; (C) “Couves from Central Portugal”; (D) “Couve Portuguesa”; (E) “Couves from Southern inland Portugal”. The three other groups correspond to: (F) cabbages; (G) miscellaneous coles; and (H) Galega kales. Landrace groups are primarily associated with morphological differences among accessions and secondly with accession geographical origin. The interannual character variation did not affect the clustering patterns of the accessions and therefore the stability of landrace classification. PCA was congruent with the landrace groups defined by the phenograms and gave supplementary information on the usefulness of the characters for the definition of the various groups.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021725
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  • 6
    Electronic Resource
    Electronic Resource
    Rotational isomers of methyl trans-cinnamate: A Raman study (1991)
    Chichester [u.a.] : Wiley-Blackwell
    Journal of Raman Spectroscopy 22 (1991), S. 519-523 
    Details
    ISSN: 0377-0486
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The Raman and infrared spectra of methyl trans-cinnamate were measured as a function of temperature in the liquid and solid phases. The temperature dependence of the band intensities established the presence of two conformers in the liquid phase (the s-cis and s-trans forms, with C=C—C=O dihedral angles equal to 0° and 180°, respectively; ΔH(s-trans)-(s-cis) = 3.43 ± 0.84 kJ mol-1) and led to the conclusion that the thermodynamically most stable s-cis form is the only form present in the solid.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jrs.1250220909
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  • 7
    Electronic Resource
    Electronic Resource
    Conformational equilibria for 3-methylstyrene: Raman and FTIR spectra and ab initio calculations (1995)
    Chichester [u.a.] : Wiley-Blackwell
    Journal of Raman Spectroscopy 26 (1995), S. 1033-1037 
    Details
    ISSN: 0377-0486
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The Raman spectra of liquid 3-methylstyrene show pairs of bands whose temperature-dependent intensities clearly suggest the occurrence of a conformational equilibrium. From these bands, assigned to cis and trans conformers, a trans-cis energy difference of 0.8 + 0.2 kJ mol-1 was obtained for the liquid phase. On the other hand, ab initio calculations at the 3-21G** and MP2/3-21G* levels predict a cis form more stable than the trans form by less than 0.15 kJ mol-1, in good agreement with gas-phase literature results.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jrs.1250261203
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  • 8
    Electronic Resource
    Electronic Resource
    Meta-substituted styrene molecules included in cydodextrins: A Raman spectroscopic study (1994)
    Chichester [u.a.] : Wiley-Blackwell
    Journal of Raman Spectroscopy 25 (1994), S. 599-605 
    Details
    ISSN: 0377-0486
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Raman spectra of styrene (S), 3-fluorostyrene (3FS), 3-chlorostyrene (3CS) and 3-methylstyrene (3MS) and their complexes in α-, β- and γ-cyclodextrins (α-, β-, γCD) were recorded at various temperatures. The substituted styrene molecules were found to provide good group frequencies, namely, vC=C and vC—H, for probing the guest molecules. Generally, the C=C oscillator is shown to be a good probe for studying conformational equilibria of the guest molecule perturbed by complex formation with the various CD molecules. The CH oscillators provide information concerning plausible structures for the complexes. On the whole, all the spectral observations are consistent with different types of structures for the (S, 3MS)-αCD and (3FS, 3CS)-αCD complexes. In particular, the Raman spectroscopic results suggest that, for 3MS-αCD the vinyl group keeps some rotational freedom. For 3FS-αCD and 3CS-αCD, it is suggested that the most electronegative fluorine and chlorine atoms are left outside the the CD cavity, with the vinyl group rotationally hindered. For the γCD complexes, the wider CD cavity sould be able to accommodate all the guest molecules considered keeping the conformer distributions of the pure liquids approximately. However, the inclusion process seems to hinder the internal rotation in such a way that even below 200 K the conformer distribution remains similar to that of the pure liquid at room temperature.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jrs.1250250721
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  • 9
    Electronic Resource
    Electronic Resource
    Raman spectra, conformational stability and normal coordinate analysis of ethylmethylamineThis paper is dedicated to Professor D. A. Long on the occasion of his 70th birthday. (1995)
    Chichester [u.a.] : Wiley-Blackwell
    Journal of Raman Spectroscopy 26 (1995), S. 653-661 
    Details
    ISSN: 0377-0486
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Raman spectra of ethylmethylamine and ethylmethylamine-N-d, both in the liquid phase, at different temperatures, and in the solid phase were recorded. Additionally, Fourier transform IR (4000 - 400 cm-1) spectra of these compounds in the liquid phase were also obtained. Normal coordinate calculations were performed for each conformer (T, G and G′), using an optimized local symmetry force field for secondary amines. These calculations allowed the assignment of the vibrational spectra, in good agreement with the experimental evidence. Whereas in the liquid phase all the three possible conformers were detected, in the solid phase only the bands ascribed to the T conformer were observed. From the temperature dependence study of the Raman spectrum of the liquid, the enthalpy differences between conformers were determined, yielding the values 4.6 ± 0.3 kJ mol-1 for ΔHG′-T′ 3.6 ± 0.3 kJ mol-1 for ΔHG - T and 1.1 ± 0.3 kJ mol-1 for ΔHG′- G. These were compared with ab initio SCF-MO results, for the isolated molecule, in order to assess the relative importance of intra-and intermolecular effects on conformational equilibria.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jrs.1250260811
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  • 10
    Electronic Resource
    Electronic Resource
    A Raman spectroscopic study of molecular interaction in long-chain primary amines systems (1982)
    Chichester [u.a.] : Wiley-Blackwell
    Journal of Raman Spectroscopy 13 (1982), S. 56-62 
    Details
    ISSN: 0377-0486
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The N—H, C—H and C—C stretching regions at 3100-3400 cm-1, 2800-3000 cm-1 and 1000-1150 cm-1, respectively, and the low-frequency accordion-like vibration region, have been investigated by Raman spectroscopy for the octyl-, nonyl-, dodecyl-, tetradecyl- and hexadecyl-normal primary amines series, at different temperatures and for different physical states. The spectra were interpreted in terms of structural changes, particularly of the conformations of the paraffin chains, the chain length and the physical state. The changes in the N—H region were associated mainly with molecular association, whereas the changes in the other spectral regions studied were associated mainly with the molecular packing of the chains.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jrs.1250130111
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