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  • 1
    ISSN: 1432-0789
    Keywords: Key wordsMacrophomina phaseolina ; Antagonists ; Germination ; Agglutination ; Pseudomonas fluorescence ; Trichoderma harzianum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The colonization of Macrophomina phaseolina sclerotia by microbial parasites was evaluated in unsterilized field soil at different levels of soil moisture (0, –5, and –10 kPa) and temperature (20, 30, and 40°C). The maximum colonization of sclerotia was recorded in soil held at –5 or –10 kPa at 30–40°C. Trichoderma harzianum isolate 25–92 and Pseudomonas fluorescens isolate 4-92 were recorded as potential sclerotial parasites, and they significantly (P=0.05) reduced the germination of sclerotia by 60–63%. Cells of P. fluorescens and buffer-washed conidia of T. harzianum were completely agglutinated at 28°C with crude agglutinin of M. phaseolina. The ability of different antagonists to parasitize the sclerotia were correlated with the agglutination ability of the antagonists.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0789
    Keywords: Macrophomina phaseolina ; Antagonists ; Germination ; Agglutination ; Pseudomonas fluorescence ; Trichoderma harzianum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The colonization of Macrophomina phaseolina sclerotia by microbial parasites was evaluated in unsterilized field soil at different levels of soil moisture (0,-5, and-10 kPa) and temperature (20, 30, and 40°C). The maximum colonization of sclerotia was recorded in soil held at-5 or-10 kPa at 30–40°C. Trichoderma harzianum isolate 25–92 and Pseudomonas fluorescens isolate 4–92 were recorded as potential sclerotial parasites, and they significantly (P=0.05) reduced the germination of sclerotia by 60–63%. Cells of P. fluorescens and buffer-washed conidia of T. harzianum were completely agglutinated at 28°C with crude agglutinin of M. phaseolina. The ability of different antagonists to parasitize the sclerotia were correlated with the agglutination ability of the antagonists.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 3
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    Journal of Chemical Technology AND Biotechnology 73 (1998), S. 23-30 
    ISSN: 0268-2575
    Keywords: vitamin C ; structured mathematical model ; continuous culture kinetics ; sorbose ; sorbitol ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The kinetics of the biotransformation of D-sorbitol to L-sorbose by Acetobacter suboxydans (NRRL B-72) features both substrate and product inhibition. Model-based reactor operating strategies can eliminate the inhibitions and improve the yield and productivity of the fermentation. Unstructured models are usually unable to acknowledge complex intracellular physiological changes and therefore their application to dynamic growth conditions is limited. By considering RNA as a bottleneck growth limiting compound a structured mathematical model was developed to describe the transient growth of Acetobacter suboxydans. The model parameters were determined by a non-linear regression technique which minimized the deviation between the model prediction and the experimental (extracellular and intracellular) continuous culture data. The model was used to simulate the transient culture behaviour during the step-down in the dilution rates (from 0·1 h-1 to 0·05 h-1) in the continuous culture experiments. The experimental results and the model simulation results showed reasonable agreement. © 1998 Society of Chemical Industry
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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