ZIB

Hits per page

hits 1 - 2 | 2 hits

Sorting

Electronic Resource

Progressive diffuse leukoencephalopathy in patients with acquired immune deficiency syndrome (AIDS) (1985)

Kleihues, P. ; Lang, W. ; Burger, P. C. ; [et al.]

Springer

Acta neuropathologica 68 (1985), S. 333-339

add to mindlist on the mindlist

Details

ISSN: 1432-0533

Keywords: Acquired immune deficiency syndrome (AIDS) ; Leukoencephalopathy ; Cytomegalovirus ; Papovavirus ; HTLV-III/LAV

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Summary Two adult patients with acquired immune deficiency syndrome (AIDS) presented with psychoorganic symptoms produced by an extensive cerebral and cerebellar leukoencephalopathy. Diffuse loss of myelin and axons with reactive astrocytosis and distinctive multinucleated giant cells were prominent in the deep white matter, but less so in the subcortical white matter and in compact myelinated pathways. Bilateral involvement of the centrum semiovale produced distal Wallerian degeneration of the descending pyramidal tracts, which in one patient correlated with progressive paraparesis and bladder dysfunction. Although there were morphological indications of cytomegalovirus infection and immunohistochemical evidence of papovavirus antigens, the neuropathology did not resemble that usually associated with infection by these opportunistic agents. The possibility is entertained that the progressive diffuse leukoencephalopathy (PDL) in these patients was directly related to infection with human T-cell lymphotropic virus (HTLV-III/LAV), the etiologic agent of AIDS.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00690837

Permalink

Library	Location	Call Number	Volume/Issue/Year	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

Electronic Resource

Preparation of biological tissue sections for correlative ion, electron, and light microscopy (1984)

Kupke, K. G. ; Pickett, J. P. ; Ingram, P. ; [et al.]

New York, NY : Wiley-Blackwell

Journal of Electron Microscopy Technique 1 (1984), S. 299-309

add to mindlist on the mindlist

Details

ISSN: 0741-0581

Keywords: Electron microscopy ; Ion microscopy ; Correlative microscopy ; Electron probe microanalysis ; Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Natural Sciences in General

Notes: In order to correctly interpret the chemical images obtained using ion microscopy (IM), it is useful to correlate them with the information provided by conventional light microscopy (LM), secondary electron imaging (SEI), backscattered electron imaging (BEI), and electron probe microanalysis (EPMA). Accordingly, we have devised a technique of specimen preparation which allows for the application of several different microanalytical techniques to a single histologic section mounted on the same substrate. Sections are cut onto polyester plastic coverslips (devoid of peaks for any element with atomic number 〉 9 using EPMA) and studied by LM. After a light rotary coating with carbon (to prevent charging), the section can then be examined by SEI, BEI, and EPMA. Specific areas can be marked for IM study either with an objective-mounted pin tissue microlocater, or by placing small pieces of metal foil, cut in specific geometric shapes, over features of interest. After sputter-coating the sample with platinum, metal-free shadows are visible using a low-power reflected light microscope available on a typical IM sample chamber as a guide for ion beam placement. The conductive coatings also minimize specimen charging during IM. Post-IM light microscopy, SEI, and BEI are used to confirm the location of specific areas probed in the IM experiments and to provide information on differential ion-sputtering artifacts and tissue contaminants. This new correlative technique should permit better understanding of the images obtained with these diverse instruments.

Additional Material: 7 Ill.