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  • Chemistry  (29)
  • Denitrification  (4)
  • Food Science, Agricultural, Medicinal and Pharmaceutical Chemistry  (3)
  • 1
    Digitale Medien
    Digitale Medien
    Copper-containing nitrite reductase from Pseudomonas aureofaciens is functional in a mutationally cytochrome cd 1-free background (NirS−) of Pseudomonas stutzeri (1993)
    Springer
    Archives of microbiology 160 (1993), S. 18-26 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Nucleotide sequence ; Apocytochrome cd 1 ; Heme d 1 incorporation ; Denitrification ; Copper coordination ; Signal peptide ; Escherichia coli
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The structural gene, nirK, for the respiratory Cu-containing nitrite reductase from denitrifying Pseudomonas aureofaciens was isolated and sequenced. It encodes a polypeptide of 363 amino acids including a signal peptide of 24 amino acids for protein export. The sequence showed 63.8% positional identity with the amino acid sequence of “Achromobacter cycloclastes” nitrite reductase. Ligands for the blue, type I Cu-binding site and for a putative type-II site were identified. The nirK gene was transferred to the mutant MK202 of P. stutzeri which lacks cytochrome cd 1 nitrite reductase due to a transposon Tn5 insertion in its structural gene, nirS. The heterologous enzyme was active in vitro and in vivo in this background and restored the mutationally interrupted denitrification pathway. Transfer of nirK to Escherichia coli resulted in an active nitrite reductase in vitro. Expression of the nirS gene from P. stutzeri in P. aureofaciens and E. coli led to nonfunctional gene products. Nitrite reductase activity of cell extract from either bacterium could be reconstituted by addition of heme d 1, indicating that both heterologous hosts synthesized a cytochrome cd 1 without the d 1-group.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00258141
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  • 2
    Digitale Medien
    Digitale Medien
    Identification of a copper protein as part of the nitrous oxide-reducing system in nitrite-respiring (denitrifying) pseudomonads (1982)
    Springer
    Archives of microbiology 132 (1982), S. 322-328 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Nitrous oxide reduction ; Copper proteins ; Cytochrome patterns ; Denitrification ; Pseudomonas
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Copper is the essential transition element for nitrous oxide respiration in Pseudomonas perfectomarinus. Two novel kinds of copper proteins were detected in this organism. Their distribution was studied under different growth conditions and in other pseudomonads, as well as their association with N2O reduction of intact cells. A low molecular mass copper protein (M r 38,000) with a single absorption band at 340 nm (oxidized form), was found only in P. perfectomarinus and was not required for N2O reduction. N2O respiration was consistently associated with a high molecular mass copper protein (M r 120,000) in P. perfectomarinus, Pseudomonas stutzeri, and in strains of Pseudomonas fluorescens that were capable of this type of respiration. The oxidized protein was violet to pink with absorption bands at 350, 480, 530, 620, and 780 nm. Pseudomonas chlororaphis and Pseudomonas aureofaciens which did not respire with N2O as electron acceptor, did not contain the novel type of copper protein. Cytochrome patterns were compared in these denitrifying pseudomonads to search for the physiological electron carrier to N2O reductase. The content and nature of the soluble c-type cytochromes depended strongly on the species and the particular growth condition.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00413383
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  • 3
    Digitale Medien
    Digitale Medien
    Characterization of the membranous denitrification enzymes nitrite reductase (cytochrome cd1) and copper-containing nitrous oxide reductase from Thiobacillus denitrificans (1996)
    Springer
    Archives of microbiology 165 (1996), S. 55-61 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Key words Cytochrome cd1 ; Nitrite reductase ; Nitrous ; oxide reductase ; Denitrification ; Thiobacillus ; denitrificans ; Pseudomonas stutzeri ; DNA hybridization
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Cytochrome cd 1-nitrite reductase and nitrous oxide reductase of Thiobacillus denitrificans were purified and characterized by biochemical and immunochemical methods. In contrast to the generally soluble nature of the denitrification enzymes, these two enzymes were isolated from the membrane fraction of T. denitrificans and remained active after solubilization with Triton X-100. The properties of the membrane-derived enzymes were similar to those of their soluble counterparts from the same organism. Nitrous oxide reductase activity was inhibited by acetylene. Nitrite reductase and nitrous oxide reductase cross-reacted with antisera raised against the soluble enzymes from Pseudomonas stutzeri. The nirS, norBC, and nosZ genes encoding the cytochrome cd 1-nitrite reductase, nitric oxide reductase, and nitrous oxide reductase, respectively, from P. stutzeri hybridized with genomic DNA from T. denitrificans. Cross-reactivity and similar N-terminal amino acid and gene sequences suggest that the primary structures of the Thiobacillus enzymes are homologous to the soluble proteins from P. stutzeri.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002030050296
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  • 4
    Digitale Medien
    Digitale Medien
    Enzyme diversity and mosaic gene organization in denitrification (1997)
    Springer
    Antonie van Leeuwenhoek 71 (1997), S. 43-58 
    Details
    ISSN: 1572-9699
    Schlagwort(e): Denitrification ; mosaic gene organization ; nitrous oxide reductase ; nitric oxide reductase ; structural models ; cytochrome c oxidase
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Denitrification is a main branch of the global nitrogen cycle. In the past ten years unravelling the underlying biochemistry and genetics has proceeded at an increasing pace. Fungal denitrification has become a new field. The biochemical investigation of denitrification has culminated in the description of the crystal structures of the two types of nitrite reductases. The N2O reductase shares with cytochrome c oxidase the CuA center as a structurally novel metal site. The cytochrome b subunit of NO reductase has a striking conservation of heme-binding transmembrane segments versus the subunit I of cytochrome c oxidase. Another putative denitrification gene product shows structural relation to the subunit III of the oxidase. N2O reductase and NO reductase may be ancestors of energy-conserving enzymes of the heme-copper oxidase superfamily. More than 30 genes for denitrification are located in a 〉30-kb cluster in Pseudomonas stutzeri, and comparable gene clusters have been identifi ed in Pseudomonas aeruginosa and Paracoccus denitrificans. Genes necessary for nitrite reduction and NO reduction have a mosaic arrangement with very few conserved locations within these clusters and relative to each other.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1000112008026
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  • 5
    Digitale Medien
    Digitale Medien
    Bildungsweise und Struktur des Pentabromocarbonat-Anions [[CBr5⊖]n - Zur Oxidation von Enaminen (1976)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 109 (1976), S. 306-313 
    Details
    ISSN: 0009-2940
    Schlagwort(e): Chemistry ; Inorganic Chemistry
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Beschreibung / Inhaltsverzeichnis: Formation and Structure of the Pentabromocarbonate Anion [CBr5⊖]n - On the Oxidation of En-aminesOxidation of enamines 5 with tetrabromomethane yields stable crystalline pentabromocarbonates 6, which may easily be converted into the corresponding perchlorates 7. From the pentabromocarbonates, tetrabromomethane cannot be extracted either by organic solvents or by heating in vacuo; with silver nitrate, however, silver bromide can be precipitated from tetraphenylphosphonium pentabromocarbonate (9). If labelled 9 - prepared from tetraphenylphosphonium [82Br]bromide and tetrabromomethane - is treated with silver nitrate, all the activity is found in the silver bromide precipitate. This is clear evidence against a trigonal bipyramidal structure of the pentabromocarbonate anion CBr5⊖]n.
    Notizen: Bei der Oxidation von Enaminen 5 mit Tetrabrommethan entstehen stabile kristalline Pentabromocarbonate 6, die sich leicht in die entsprechenden Perchlorate 7 überführen lassen. Aus den Pentabromocarbonaten kann Tetrabrommethan weder durch Extraktion mit organischen Solventien noch durch Erhitzen i. Vak. abgespalten werden, mit Silbernitrat kann jedoch aus Tetraphenylphosphonium-pentabromocarbonat (9)Silberbromid ausgefällt werden. Bei der Umsetzung von markiertem 9 - dargestellt aus [82Br] Tetraphenylphosphoniumbromid und Tetrabrommethan - mit Silbernitrat findet man die Gesamtaktivität im Silberbromid-Niederschlag. Dies spricht gegen die Struktur einer trigonalen Bipyramide für das CBr5⊖]n-Anion.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/cber.19761090130
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  • 6
    Digitale Medien
    Digitale Medien
    Synthesis and crystal structure of 2,6-dibromo-3,7-dimethyl-1H,5H-pyrazolo[1,2-a]Pyrazole-1,5-dione (1986)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 328 (1986), S. 648-650 
    Details
    ISSN: 0021-8383
    Schlagwort(e): Chemistry ; Organic Chemistry
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Zusätzliches Material: 1 Tab.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/prac.19863280428
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  • 7
    Digitale Medien
    Digitale Medien
    Prediction of the properties of model polymer solutions and blends (1994)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 40 (1994), S. 878-887 
    Details
    ISSN: 0001-1541
    Schlagwort(e): Chemistry ; Chemical Engineering
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: In this work we present an extension of the statistical associating fluid theory (SAFT) developed for associating fluids by applying this extension to polymer solutions and blends. The polymer molecule is modeled as a flexible chain made of bonded spherical segments. These segments interact with the Lennard-Jones potential. The extension of SAFT is compared with molecular simulation results for polymer solutions and blends. Since the same force model is used in the simulation and theory, this comparison is a strong test of the assumptions made in deriving the theory. Results are presented for pure polymers of up to 50 segments long, polymer solutions of a polymer 20 segments long dissolved in its own monomer, and blends of polymers of 25 segments each. The theory produces results that are in better agreement with simulation results than the Flory-Huggins theory for the systems studied.
    Zusätzliches Material: 9 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/aic.690400514
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  • 8
    Digitale Medien
    Digitale Medien
    A therotical basis for the “solvent effect” (1978)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 1 (1978), S. 275-276 
    Details
    ISSN: 0935-6304
    Schlagwort(e): Gas Chromatography ; Capillary ; Splitless injection ; Theoretical basis for reconcetration by solvent effects ; Guidelines for an effective solvent effect, in literature citation ; Chemistry ; Analytical Chemistry and Spectroscopy
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Zusätzliches Material: 1 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/jhrc.1240010513
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  • 9
    Digitale Medien
    Digitale Medien
    Kathodische Korrosion von Blei im Erdboden (1969)
    Weinheim [u.a.] : Wiley-Blackwell
    Materials and Corrosion/Werkstoffe und Korrosion 20 (1969), S. 578-583 
    Details
    ISSN: 0947-5117
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Maschinenbau
    Beschreibung / Inhaltsverzeichnis: Cathodic corrosion of lead in the soilCathodic corrosion of lead in the soil can be expected in exceptional cases only. The corrosion found on a particular lead cable could be simulated in the laboratory. It could be confirmed, that  -  probably by decomposition of primary lead hydride  -  metallic lead is deposited below layers of Ca and Mg compounds already at potentials below -1,7 V. A special switching method was developed to account for breakthrough polarisation.
    Notizen: Kathodische Korrosion von Blei im Boden ist grundsätzlich nur in Ausnahmefällen zu erwarten. Diese an einem Kabel gefundene Korrosion wurde im Laboratorium nachgebildet. Hierbei konnte bestätigt werden, daß unter Deckschichten aus Calcium- und Magnesiumverbindungen bei Potentialen schon unter -1,7 V metallisches, Blei abgeschieden wurde, wahrscheinlich durch Zersetzung von primär entstandenem Bleihydrid. Für derartige Untersuchungen wurde eine spezielle Schaltmethode entwickelt, bei welcher auch die Durchtrittspolarisation berücksichtigt wird.
    Zusätzliches Material: 8 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/maco.19690200704
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  • 10
    Digitale Medien
    Digitale Medien
    Kunststoffe im Automobilbau - Anwendung und Wiederverwertung (1991)
    Weinheim : Wiley-Blackwell
    Materialwissenschaft und Werkstofftechnik 22 (1991), S. 275-278 
    Details
    ISSN: 0933-5137
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Maschinenbau
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/mawe.19910220710
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