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  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 148 (1987), S. 107-114 
    ISSN: 1432-072X
    Schlagwort(e): Autotrophy ; Clostridia ; Clostridium aceticum ; Plasmids
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A plasmid screening was performed on 150 strains out of 75 clostridial species using a modification of the alkaline-lysis procedure. In 26 strains representing 21 species one or more plasmid bands were detected ranging in size from 3 to more than 100 kilobase pairs. Clostridium aceticum proved to contain a single small plasmid (pCA1) of 5.4 kbp as revealed by restriction analysis and electron microscopy. A physical map of pCA1 has been constructed. Spontaneous mutants of C. aceticum defective in autotrophic growth have been isolated. No direct correlation between plasmid content and autotrophy could be found.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 128 (1981), S. 288-293 
    ISSN: 1432-072X
    Schlagwort(e): Clostridium aceticum ; Acetic acid formation ; Chemolithotrophic growth ; Hydrogen uptake
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Spores of Clostridium aceticum (Wieringa) from 1947 have been revived, and a study for a redescription of this microorganism has been carried out. C. aceticum was Gram negative. The cells were rodshaped and peritrichously flagellated. Round spores were formed in terminal position. The DNA contained 33 mol % guanine plus cytosine. The organism was obligately anaerobic and grew either chemolithotrophically with H2+CO2 or chemoorgranotrophically with compounds such as fructose, l-glutamate, l-malate or pyruvate. H2 and CO2 were converted to acetic acid according to the following equation: $$2{\text{ CO}}_{\text{2}} + 4{\text{H}}_{\text{2}} \to {\text{CH}}_{\text{3}} {\text{COOH + 2H}}_{\text{2}} {\text{O}}$$ The optimal temperature for growth was 30°C. The optimal pH for chemolithotrophic growth was 8.3. The doubling times for chemolithotrophic and chemoorganotrophic growth were 25 and 8h, respectively.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 128 (1981), S. 294-298 
    ISSN: 1432-072X
    Schlagwort(e): Acetobacterium woodii ; Clostridium aceticum ; Mixotrophic growth ; H2 utilization ; CO2 fixation ; H2 inhibition of chemoorganotrophic growth
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract During growth of Acetobacterium woodii on fructose, glucose or lactate in a medium containing less than 0.04% bicarbonate, molecular hydrogen was evolved up to 0.1 mol per mol of substrate. Under an H2-atmosphere growth of A. woodii with organic substrates was completely inhibited whereas under an H2/CO2-atmosphere rapid growth occurred. Under these conditions H2+CO2 and the organic substrate were utilized simultaneously indicating that A. woodii was able to grow mixotrophically. Clostridium aceticum differed from A. woodii in that H2 was only evolved in the stationary phase, that the inhibition by H2 was observed at pH 8.5 but not at pH 7.5, anf that in the presence of fructose and H2+CO2 only fructose was utilized. The hydrogenase activity of fructose-grown cells of C. aceticum amounted to only 12% of that of H2+CO2-grown cells. With A. woodii a corresponding decrease of the activity of this enzyme was not observed.
    Materialart: Digitale Medien
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 70 (1985), S. 207-212 
    ISSN: 1432-2242
    Schlagwort(e): Pisum mutants and recombinants ; Phytotron experiments ; Gene-ecology ; Temperature
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The flowering behaviour of 17 Pisum mutants and 20 recombinants was studied under three different temperatures using long-day phytotron conditions. A constant low temperature of 12.5 ° C led to a strong delay in flowering in all the genotypes tested but distinct relative differences could be found between them. Relative differences were also present with regard to speed of ontogenetic development under a permanent high temperature of 25.5 °C or under an alternating change between low and high temperature. Under the low temperature, recombinants R 20D and R 20E, carrying gene efr for earliness, entered the flowering period more than 4 weeks later than the donor of efr, demonstrating thereby a negative influence of one of the other mutant genes on efr. The high temperature of 25 °C influenced the flowering behaviour of 4 fasciated genotypes negatively — in contrast to the other strains studied. The plants of recombinant R 405 produced only tiny flower buds under these conditions. None of the plants of recombinant R 142F flowered under either the constant low or high temperature — they need the change of low and higher temperature for normal flower formation. The experiments show that most of the genotypes tested react specifically to the three temperature conditions offered to them.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 75 (1988), S. 344-349 
    ISSN: 1432-2242
    Schlagwort(e): Pisum mutants and recombinants ; Phytotron experiments ; Gene-ecology ; Photoperiod ; Suppressor genes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The flowering behavior of 59 Pisum mutants and 228 recombinants was studied in the phytotron in four different photoperiods (continuous light, long-day 18/6 h, short-day 12/12 h, extreme short-day 6/18 h). There was no or little difference in the response of the genotypes to long-day and permanent light, whereas great differences were observed between long- and short-day 12/12 h and between the two short-day trials. About half the genotypes tested were unable to survive or to flower in extreme short-day. Some recombinants, however, had an almost normal development under these unfavorable conditions. Gene fis controls the photoperiodic reaction of the plants: they are unable to flower in short-day. Gene fds negatively influences gene efr for earliness: it causes a strong delay of flowering of efr recombinants in long-day and suppresses the formation of functionable flowers in short-day. Most of the genotypes tested showed a specific reaction to the four photoperiods different from that of the mother variety and the other genotypes. The practical aim of our phytotron experiments is the preselection of Pisum genotypes which might be suited for cultivation in countries with short-day climate.
    Materialart: Digitale Medien
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  • 6
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 56 (1980), S. 71-79 
    ISSN: 1432-2242
    Schlagwort(e): Gene-ecology ; Fasciated mutants ; Penetrance ; Flowering behaviour ; Seed production
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Six mutants and nine recombinants of Pisum sativum were grown along with the mother variety at Kurukshetra, North India. The findings obtained were compared with those obtained for the same material grown at Bonn, Germany. The following observations were made. Stem length and degree of branching are influenced differentially in the various genotypes tested in India as a consequence of a specific reaction of the genes to the climatic conditions. A gene for weak stem fasciation and gene efr for earliness in a specific gene combination are unable to express their action in North India whereas they are fully active in Germany. Furthermore, in Kurukshetra early flowering of some recombinants does not result in early ripening because their seeds require about double the time for full ripening than those of the mother variety. At Kurukshetra, recombinant R 674A proved to be highly heat susceptible. All the plants died in early stages of ontogenetic development. Four other genotypes died due to heat before completing seed ripening. One mutant and three recombinants were found to be more tolerant to powdery mildew attack than the mother variety and Indian local lines. The seed production of eight genotypes in relation to that of the initial line was essentially better in North India than in Germany. They are obviously better adapted to the semi-arid conditions. Some of them appear to be useful for pea breeding in India. In contrast, a fasciated mutant, high yielding in Germany, is not able to express this potentiality at Kurukshetra. At Udaipur (Rajasthan, Western India), this mutant is unable to flower. Another four genotypes, tested at both Indian locations, exhibited an essentially poorer seed production at Udaipur than at Kurukshetra due to some ecological factors. The findings indicate a specific response of some of the genotypes tested to the specific ecological conditions of the three locations, their response differing from that of the mother variety demonstrating thereby a different adaptational optimum.
    Materialart: Digitale Medien
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