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  • 1
    Electronic Resource
    Electronic Resource
    Selenium requirement for active xanthine dehydrogenase from Clostridium acidiurici and Clostridium cylindrosporum (1979)
    Springer
    Archives of microbiology 121 (1979), S. 255-260 
    Details
    ISSN: 1432-072X
    Keywords: Purine fermentation ; Xanthine dehydrogenase ; Selenium ; Tungsten ; Molybdenum ; Clostridium acidiurici ; Clostridium cylindrosporum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The xanthine dehydrogenase of Clostridium acidiurici and C. cylindrosporum was assayed with methyl viologen as acceptor. In C. acidiurici the basal activity level was about 0.3 μmol/min x mg of protein. Cells grown on uric acid in the presence of 10-7 M selenite showed a 14-fold increase in xanthine dehydrogenase activity, which decreased with higher selenite concentrations (10-5 M). The supplementation with 10-7 M molybdate or tungstate was without effect. High concentrations of tungstate decreased the xanthine dehydrogenase if selenite was also present. In comparison, high concentrations of molybdate affected only a small decrease in activity level at the optimal concentration for selenite and relieved to some degree the inhibitory effect of 10-5 M selenite. With hypoxanthine and xanthine as substrates for growth again only the addition of selenite was necessary to show a similar increase in xanthine dehydrogenase activity. C. acidiurici could be grown in a mineral medium. Both xanthine dehydrogenase and formate dehydrogenase exhibited the highest level of activity if selenite and tungstate were present in that medium. In C. cylindrosporum the basal activity level of xanthine dehydrogenase was about 0.95 μmol/min x mg of protein. The addition of 10-7 M selenite to the growth medium increased the activity level about 3-fold, but the highest level (3.7 U/mg) was reached if 10-7 M molybdate was also added. The presence of tungstate resulted in a decreased enzyme activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425064
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  • 2
    Electronic Resource
    Electronic Resource
    Comparative studies on physiology and taxonomy of obligately purinolytic clostridia (1984)
    Springer
    Archives of microbiology 138 (1984), S. 345-353 
    Details
    ISSN: 1432-072X
    Keywords: Clostridium acidiurici ; Clostridium cylindrosporum ; Clostridium purinolyticum ; Purine metabolism ; Selenite ; Antibiogram ; DNA homology ; Taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Eleven strains of obligately purinolytic clostridia have been studied with respect to their assignment to the three type strains of Clostridium acidiurici, C. cylindrosporum, and C. purinolyticum. DNA/DNA-hybridization proved to be the method of choice for differentiation whereas phenotypic characteristics such as spore morphology, substrate spectra, nutritional requirements, product formation, and sensitivity against various antibiotics did not allow unequivocal identification. All strains depended on selenite for growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00410902
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  • 3
    Electronic Resource
    Electronic Resource
    Accumulation and incorporation of 185W-tungsten into proteins of Clostridium acidiurici and Clostridium cylindrosporum (1987)
    Springer
    Archives of microbiology 147 (1987), S. 295-299 
    Details
    ISSN: 1432-072X
    Keywords: Clostridium acidiurici ; Clostridium cylindrosporum ; Tungsten uptake ; Molybdate antagonism ; Formate dehydrogenase ; Tungsten-binding-storage protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Uptake of tungstate by growing cells was unaffected by the presence of molybdate in Clostridium cylindrosporum, whereas in C. acidiurici the accumulation was decreased by molybdate at 10-6 mol/l tungstate and higher concentrations. The labelling pattern of soluble proteins by 185W-tungsten indicated after gel chromatography the presence of three different tungstoproteins in both bacteria. Formate dehydrogenase activity always eluted at a maximum of tungsten labelling. The incorporation of tungsten into formate dehydrogenase containing fractions and a possible tungsten-binding-storage protein was independent of the presence of excess molydate pointing to a genuine role for tungstate in these bacteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00463491
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  • 4
    Electronic Resource
    Electronic Resource
    Protonierung von Purin, Adenin und Guanin NMR.-Spektren und Strukturen der Mono-, Di- und Tri-KationenProtonenresonanzspektren von Pteridinen und Purinen, VIII. Mitteilung; VII. Mitt. vgl. [1]. (1971)
    New York, NY : Wiley-Blackwell
    Helvetica Chimica Acta 54 (1971), S. 1543-1558 
    Details
    ISSN: 0018-019X
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Proton NMR. spectra of purine, adenine, guanine and methylsubstituted guanines have been measured in CF3COOH, FSO3H and FSO3H—SbF5—SO2 at 27° and low temperatures. These conditions permit to study multiple protonation of purines, using chemical shifts of CH, NH and OH protons. The spectra of mono-, di- and tri-cations are described and fully assigned.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/hlca.19710540604
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  • 5
    Electronic Resource
    Electronic Resource
    Protonierung von Amino- und Hydroxypyrimidinen NMR-Spektren und Strukturen der Mono- und DikationenProtonenresonanzspektren von Pteridinen, VII. Mitteilung VI vgl. [1]. (1970)
    New York, NY : Wiley-Blackwell
    Helvetica Chimica Acta 53 (1970), S. 299-320 
    Details
    ISSN: 0018-019X
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Proton-NMR. spectra of amino- and hydroxypyrimidines including biologically important bases have been measured in four solvents: CF3COOH, CF3COOH—SO2, FSO3H and FSO3H—SbF5-SO2 at 27° and -55°C. In CF3COOH mono-cations are formed, whereas in FSO3H and FSO3H—SbF5-SO2 double protonation occurs. In each case the structures of the protonated species are derived from the chemical shifts of CH, NH and OH protons and proton-proton spin coupling constants. A combination of the measurements described leads to a complete assignment of all proton resonances of the protonated pyrimidines. This approach is also recommended for the structural determination of heterocyclic compounds.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/hlca.19700530210
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  • 6
    Electronic Resource
    Electronic Resource
    Ueber die reducirende Wirkung der Soda bei Löthrohrversuchen (1850)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 49 (1850), S. 191-192 
    Details
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/prac.18500490118
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  • 7
    Electronic Resource
    Electronic Resource
    Löslichkeit des Chlornatriums in Alkohol (1847)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 40 (1847), S. 448-448 
    Details
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/prac.184704001107
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  • 8
    Electronic Resource
    Electronic Resource
    Löslichkeit des Chlornatriums in Alkohol (1848)
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 64 (1848), S. 293-293 
    Details
    ISSN: 0075-4617
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jlac.18480640317
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