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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Transplant international 11 (1998), S. S357 
    ISSN: 1432-2277
    Keywords: Key words MHC ; Hepatocyte ; CTL ; Gene transfer ; Liver
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Previous studies suggest liver transplants can protect other transplanted organs. This effect may be mediated by hepatocytes secreting large amounts of soluble MHC class I antigen. Here our aim was to determine whether immunologic priming by membrane-bound alloantigen could be inhibited by allospecific antigen produced in a secreted form. Cultured Lewis (RT1.Al) hepatocytes were transfected with plasmids encoding either a membrane-bound or secreted form of the alloantigen, RT1.Aa. Cytotoxic T-lymphocyte (CTL) precursor assays were performed on Lewis splenocytes cultured with transfected hepatocytes, or hepatocytes were injected into the portal vein of prospective Lewis recipients of an ACI (RT1.Aa) liver allograft. Results showed CTL priming by membrane-bound RT1.Aa was inhibited in vitro by soluble RT1.Aa. Similarly, acceleration of ACI allograft rejection induced by membrane-bound antigen was abrogated following co-injection of hepatocytes secreting donor alloantigen. In conclusion, production of soluble donor alloantigen by liver transplants may provide protection against the alloimmune response.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1435-2451
    Keywords: Colon suture ; Leakage ; Fibrin gluing ; Colon-Naht ; Nahtinsuffizienz ; Fibrinklebung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Im Vergleich zum übrigen Gastrointestinaltrakt ist die Chirurgie am Colon mit einer unverhältnismäßig hohen Rate an Anastomoseninsuffizienzen behaftet. Die Wertigkeit verschiedener Techniken der zusätzlichen Gewebeklebung (Fibrinklebung mit Tissucol auch mit Kollagenvlies; Gentamycin-haltiges Kollagen-Fibrinogen-Lyophilisat) wurde am Rattencolon untersucht. In der postoperativen Frühphase (−24 h p.op.) erreichen die zusätzlich mit den Klebetechniken versorgten Nähte statistisch signifikant höhere mechanische Belastbarkeit. Bei den Kollagengeweben wird eine die Wundheilung offensichtlich nicht störende Fremdkörperreaktion beobachtet. Der Kollagen-Fibrinogen-Verbund bietet bei Aminoglycosidzusatz den Vorteil des lokalen Antibioticumschutzes.
    Notes: Summary Colorectal surgery is still burdened by a comparatively high rate of anastomotic leakages. The benefits of different fibrin gluing techniques (fibrin adhesive Tissucol, also with collagen tissue; gentamicinincorporated collagen-fibrinogen lyophilisate) were studied in the rat colon. In the early postoperative course (up to 24h p.o.), the sutures that were also sealed by the gluing techniques showed a statistically significant capacity to withstand more stress. In the collagen tissues, the foreign body reaction, has no side effects on wound healing. The gentamicin-collagen-fibrogen tissue has the advantage of local antibiotic protection.
    Type of Medium: Electronic Resource
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