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  • 1
    Electronic Resource
    Electronic Resource
    Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification (1990)
    Springer
    Plant cell reports 9 (1990), S. 30-33 
    Details
    ISSN: 1432-203X
    Keywords: Cryopreservation ; Vitrification ; Nucellar cells ; Navel orange ; Citrus sinensis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The nucellar cells of navel orange(Citrus sinensis Osb. var. brasiliensis Tanaka) were successfully cryopreserved by vitrification. In this method, cells were sufficiently dehydrated with highly concentrated cryoprotective solution(PVS2) prior to direct plunge in liquid nitrogen. The PVS2 contains(w/v) 30% glycerol, 15% ethylene glycol and 15% DMSO in Murashige-Tucker medium(MT) containing 0.15 M sucrose. Cells were treated with 60% PVS2 at 25°C for 5 min and then chilled PVS2 at 0°C for 3 min. The cell suspension of about 0.1 ml was loaded in a 0.5 ml transparent plastic straw and directly plunged in liquid nitrogen for 30 min. After rapid warming, the cell suspension was expelled in 2 ml of MT medium containing 1.2 M sucrose. The average rate of survival was about 80%. The vitrified cells regenerated plantlets. This method is very simple and the time required for cryopreservation is only about 10 min.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232130
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Survival of cultured cells and somatic embryos of Asparagus officinalis cryopreserved by vitrification (1989)
    Springer
    Plant cell reports 8 (1989), S. 418-421 
    Details
    ISSN: 1432-203X
    Keywords: Cryopreservation ; Vitrification ; Plant germplasm ; Asparagus ; Asparagus officinalis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cultured cells and somatic embryos derived from the mesophyll tissue of asparagus (Asparagus officinalis L.) were cryopreserved by vitrification. The vitrification solution (PVS) contains (w/v) 22% glycerol, 15% ethylene glycol, 15% propylene glycol and 7% DMSO in Murashige-Skoog medium enriched with 0.5M sorbitol. After initial cryoprotection with sorbitol supplemented MS medium containing 12% ethylene glycol, cells or embryos were exposed stepwise to 85% PVS at 0°C. They were loaded into 0.5 ml transparent straws, and were then plunged directly into liquid nitrogen. After rapid warming, PVS was removed and diluted stepwise. The highest survivals of vitrified cells and embryos were about 65 and 50%, respectively. Surviving embryos developed into plantlets.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00270083
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Cryopreservation of in vitro-cultured multiple bud clusters of asparagus (Asparagus officinalis L. cv Hiroshimagreen (2n=30) by the techniques of vitrification (1992)
    Springer
    Plant cell reports 11 (1992), S. 433-437 
    Details
    ISSN: 1432-203X
    Keywords: Cryopreservation ; Vitrification ; Asparagus ; In vitro ; cultured bud clusters
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A culture line of asparagus forming green bulbous structures consisting of numerous multiple bud clusters designated “bud clusters” was induced from a meristem culture of asparagus (Asparagus officinalis L.cv. Hiroshimagreen, 2n=30). Small cubic segments (2 mm3) cut from bud clusters were cryopreserved using three different cryogenic protocols. Only vitrification produced very high levels of shoot formation after cooling to −196°C. Segments were treated with a vitrification solution (PVS2) at 25°C for 45 min or at 0°C for 120 min prior to a direct plunge into liquid nitrogen. After rapid warming, the segments were expelled into Murashige and Skoog medium containing 1.2 M sucrose for 10 min and then plated on agar shoot outgrowth medium. The average rate of shoot formation of vitrified segments producing normal shoots was near 90% without any preculture and/or cold-acclimation treatment. Revived segments resumed growth within 3 days and developed about three shoots per segment. In vitro-cultured bud clusters appear promising as material for cryopreserving asparagus germplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232685
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Cryopreservation of zygotic embryos of a Japanese terrestrial orchid (Bletilla striata) by vitrification (1997)
    Springer
    Plant cell reports 16 (1997), S. 754-757 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsBletilla striata ; Cryopreservation ; Embryo ; Orchid ; Vitrification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The seeds of a Japanese terrestrial orchid (Bletilla striata Rchb.f.) were germinated and cultured on solidified new Dogashima (ND) medium for 10 days. These embryos were then precultured on ND medium supplemented with 0.3 m sucrose for 3 days at 25°C in continuous dark. The embryos were then overlaid with a mixture of 2 m glycerol and 0.4 m sucrose for 15 min at 25°C and finally dehydrated with highly concentrated vitrification solution (PVS2) for 3 h at 0°C prior to immersion into liquid nitrogen for 30 min. After rapid warming, the embryos were washed with liquid ND medium supplemented with 1.2 m sucrose for 20 min and then plated on ND medium. Successfully vitrified and warmed embryos developed into normal plantlets. The rate of plant regeneration amounted to about 60%. This vitrification method appears to be a promising technique for cryopreservation of orchids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050314
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  • 5
    Electronic Resource
    Electronic Resource
    Cryopreservation of invitro-grown shoot tips of taro (Colocasia esculenta (L.) Schott) by vitrification. 1. Investigation of basic conditions of the vitrification procedure (1997)
    Springer
    Plant cell reports 16 (1997), S. 594-599 
    Details
    ISSN: 1432-203X
    Keywords: Shoot tips ; Cryopreservation ; Vitrification ; Taro [Colocasia esculenta (L.) Schott.] ; Tropical crops
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Invitro-grown shoot tips of taro (Colocasia esculenta (L.) Schott.) were successfully cryopreserved by vitrification. Excised shoot tips precultured on solidified MS supplemented with 0.3M sucrose and maintained under a 16 h phtoperiod at 25°C for 16 h were loaded with a mixture of 2M glycerol plus 0.4M sucrose for 20 min at 25°C. The shoot tips were then sufficiently dehydrated with a highly concentrated vitrification solution (PVS2) for 20 min at 25°C prior to immersion into liquid nitrogen. Successfully vitrified and warmed shoot tips resumed growth within 7 days and developed shoots directly without intermediate callus formation. The average rate of shoot recovery amounted to around 80%, and the vitrification protocol appeared to be very promising for the cryopreservation of taro germplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01275498
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  • 6
    Electronic Resource
    Electronic Resource
    Cryopreservation of encapsulated shoot primordia induced in horseradish (Armoracia rusticana) hairy root cultures (1997)
    Springer
    Plant cell reports 16 (1997), S. 469-473 
    Details
    ISSN: 1432-203X
    Keywords: Cryopreservation ; Encapsulation-dehydration ; Encapsulation-vitrification ; Hairy roots ; Horseradish shoot primordia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Shoot primordia induced inArmoracia rusticana Gaertn. Mey. et Scherb. (horseradish) hairy root cultures were successfully cryopreserved by two cryogenic procedures. Encapsulated shoot primordia were precultured on solidified Murashige-Skoog medium supplemented with 0.5M sucrose for 1 day and then dehydrated with a highly concentrated vitrification solution (PVS2) for 4 h at 0°C prior to a plunge into liquid nitrogen. The survival rate of encapsulated vitrified primordia amounted to 69%. In a revised encapsulation-dehydration technique, the encapsulated shoot primordia were precultured with a mixture of 0.5M sucrose and 1M or 1.5M glycerol for 1 day to induce dehydration tolerance and then subjected to air-drying prior to a plunge into liquid nitrogen. The survival rate of encapsulated dried primordia was more than 90%, and the revived primordia produced shoots within 2 weeks after plating. A long-term preservation of shoot primordia was also achieved by the technique. Thus, this revised encapsulation-dehydration technique appears promising as a routine method for the cryopreservation of shoot primordia of hairy roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01092768
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Cryopreservation of in vitro-grown shoot tips of taro (Colocasia esculenta (L.) Schott) by vitrification. 1. Investigation of basic conditions of the vitrification procedure (1997)
    Springer
    Plant cell reports 16 (1997), S. 594-599 
    Details
    ISSN: 1432-203X
    Keywords: Key words Shoot tips ; Cryopreservation ; Vitrification ; Taro [Colocasia esculenta (L.) Schott.] ; Tropical crops
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In vitro-grown shoot tips of taro (Colocasia esculenta (L.) Schott.) were successfully cryopreserved by vitrification. Excised shoot tips precultured on solidified MS supplemented with 0.3 M sucrose and maintained under a 16 h phtoperiod at 25°C for 16 h were loaded with a mixture of 2 M glycerol plus 0.4 M sucrose for 20 min at 25°C. The shoot tips were then sufficiently dehydrated with a highly concentrated vitrification solution (PVS2) for 20 min at 25°C prior to immersion into liquid nitrogen. Successfully vitrified and warmed shoot tips resumed growth within 7 days and developed shoots directly without intermediate callus formation. The average rate of shoot recovery amounted to around 80%, and the vitrification protocol appeared to be very promising for the cryopreservation of taro germplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050285
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    Paper (German National Licenses)
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