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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell reports 12 (1993), S. 347-351 
    ISSN: 1432-203X
    Keywords: Agrobacterium-mediated transformation ; Cyphomandra betacea ; pKIWI110 ; Regeneration ; Tamarillo
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Media were developed to regenerate shoots from leaf pieces of tamarillo (Cyphomandra betacea (Cav.) Sendtner). Shoots were derived via organogenesis and could be easily rooted and transferred to the growth chamber. Transgenic tamarillo plants were produced using the binary vector pKIWI110 in the avirulent Agrobacterium strain LBA4404. All transgenic plants were kanamycin resistant and some plants expressed the β D-glucuronidase (gusA) reporter gene and were chlorsulfuron resistant. Molecular evidence for transformation was obtained using PCR (polymerase chain reaction) and Southern hybridization. Inheritance of the transgenic phenotypes was demonstrated in seedling progeny.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant systematics and evolution 205 (1997), S. 111-124 
    ISSN: 1615-6110
    Keywords: Ericales ; Actinidiaceae ; Actinidia ; A. deliciosa ; A. chinensis ; A. chrysantha ; A. arguta ; Kiwifruit ; allopolyploid ; phylogeny ; polygalacturonase (PG)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The genetic origin of kiwifruit (Actinidia deliciosa var.deliciosa) was studied using phylogenetic analysis of DNA sequences derived from the polygalacturonase gene. Results indicate that hexaploid kiwifruit had an allopolyploid origin with the diploidA. chinensis contributing one genome (genome A) and another (as yet unidentified) diploid species contributing a second genome (genome B). The results leave open the question of whether a third, distinct species contributed to the hexaploid kiwifruit genome. A tetraploid race ofA. chinensis is also suggested to be allopolyploid containing genomes A and B.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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