Electronic Resource
Springer
Plant cell reports
12 (1993), S. 347-351
ISSN:
1432-203X
Keywords:
Agrobacterium-mediated transformation
;
Cyphomandra betacea
;
pKIWI110
;
Regeneration
;
Tamarillo
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Media were developed to regenerate shoots from leaf pieces of tamarillo (Cyphomandra betacea (Cav.) Sendtner). Shoots were derived via organogenesis and could be easily rooted and transferred to the growth chamber. Transgenic tamarillo plants were produced using the binary vector pKIWI110 in the avirulent Agrobacterium strain LBA4404. All transgenic plants were kanamycin resistant and some plants expressed the β D-glucuronidase (gusA) reporter gene and were chlorsulfuron resistant. Molecular evidence for transformation was obtained using PCR (polymerase chain reaction) and Southern hybridization. Inheritance of the transgenic phenotypes was demonstrated in seedling progeny.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00237433
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