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  • 1
    Electronic Resource
    Electronic Resource
    Somaclonal variation versus chemically induced mutagenesis in tomato (Lycopersicon esculentum L.) (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 733-738 
    Details
    ISSN: 1432-2242
    Keywords: Tomato ; Somaclonal variation ; Chemical mutagenesis ; Mutants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A comparison was made of the type and frequency of mutational events found in the progeny of tomato plants regenerated after one passage in vitro with those induced by chemical mutagenesis with ethyl methane sulphonate. Several mutants were recovered in the progeny of regenerated and mutagenized plants of two cultivars of tomato. They can be grouped into the following categories: seedling lethality, male sterility, resistance to Verticillium, short stature, change in number of lateral shoots or in leaf shape. The results indicate that the two sources of variability differ in their effect, changing the spectrum and frequency of the mutants as well as, at least in some cases, their pattern of segregation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00247550
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Nuclear DNA amplification in cultured cells of Oryza sativa L. (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 65-70 
    Details
    ISSN: 1432-2242
    Keywords: DNA amplification ; Cultured cells ; Dot hybridization ; Oryza sativa L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Highly repeated nuclear DNA sequences from suspension cultured cells of Oryza sativa L. cv. ‘Roncarolo’ have been cloned in pBR322. Ten clones with specific digestion patterns have been randomly selected. Nine sequences appear to be organized in a clustered tandem array while one is interpersed in the rice genome. The clones have been used to gather information on: (a) their modulation in cultured cells as compared to whole plant and (b) their distribution in different rice cultivars belonging to the Japonica or Indica subspecies of Oryza sativa L. Hybridization with nuclear DNA isolated either from suspension or from seedlings of the ‘Roncarolo’ cultivar revealed extensive quantitative variations, with most cloned sequences showing amplification (up to 75-fold) in cultured cells. Hybridization with nuclear DNA isolated from seedlings or suspension cultured cells from different cultivars belonging to the Japonica or to the Indica sub-species of O. sativa have shown that (a) amplification also occurs in a similar pattern in the case of DNA from the other tested suspension cultured cell types but not in the case of DNA from seedlings; (b) in some cases the tested sequences show minor but significant variations in different rice accessions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00290085
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The histogenesis of somaclones from tomato (Lycopersicon esculentum Mill.) cotyledons (1988)
    Springer
    Protoplasma 142 (1988), S. 156-163 
    Details
    ISSN: 1615-6102
    Keywords: Tomato ; Lycopersicon esculentum ; Somaclonal variation ; Plant regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A histological study ofin vitro cultured cotyledonary expiants of tomato (Lycopersicon esculentum) was performed in order to determine the site (differentiated tissue or developing callus) and the mode of plant regeneration. Results have shown that callus develops at the excision sites of cotyledonary expiants and that shoots are formed exclusively within the unorganized callus: excision areas are the only morphogenetic sites and the proximal excision is the preferred site for plant regeneration. Shoots differentiate by organogenesis within the superficial region of the callus. Few neocambial cells cooperate in the neoformation. Origin from a single cell is highly unlikely since rarely observed single activated cells never developed into shoots. Regenerated plants may be chimeras if invitro culture induces genetic diversity in the initial cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01290872
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  • 4
    Electronic Resource
    Electronic Resource
    A repeated chromosomal DNA sequence is amplified as a circular extrachromosomal molecule in rice (Oryza sativa L.) (1990)
    Springer
    Molecular genetics and genomics 222 (1990), S. 58-64 
    Details
    ISSN: 1617-4623
    Keywords: Bal31 ; DNA amplification ; Extrachromosomal DNA ; Pulsed field gel electrophoresis ; Rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The plasmid pE10 is a pBR322-derived plasmid carrying a 4.5 kb rice (Oryza sativa L.) repeated DNA sequence. The cloned sequence has been shown to be amplified in cultured rice cells. The analysis of practically intact chromosomal rice DNA molecules by pulsed field gel electrophoresis has now shown that the amplification is associated with the appearance of extrachromosomal molecules. In fact, pE10 hybridizes exclusively with unfractionated DNA from leaf protoplasts, while it recognizes predominantly an extrachromosomal DNA molecule (ECD) of about 45 kb and its multiples in the case of protoplasts from cultured cells. Insensitivity to the action of the exonuclease Bal31 suggests that the molecule is circular. Analysis of restriction endonuclease products with both standard horizontal and pulsed field gel electrophoresis suggest that the extrachromosomal DNA, and its chromosomal counterpart, is composed of tandemly repeated units of about 7 kb. Thus, the smaller extrachromosomal circle should contain 6–7 repeats, while the sequence cloned in pE10 is a subset of this repeat. The extrachromosomal DNA represents about 1 % of total rice DNA and its level of amplification is not affected by the different phases of growth in culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00283023
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    Paper (German National Licenses)
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