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  • 1990-1994  (5)
  • GFAP  (3)
  • Ependymocytes  (1)
  • sulpiride-treated rats
  • 1
    Digitale Medien
    Digitale Medien
    Distribution and characteristics of the different astroglial cell types in the adult lizard (Lacerta lepida) spinal cord (1990)
    Springer
    Anatomy and embryology 181 (1990), S. 567-575 
    Details
    ISSN: 1432-0568
    Schlagwort(e): Astrocyte ; Radial astrocyte ; GFAP ; Electron microscopy ; Phylogeny
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The astroglial cells have been studied in the lizard spinal cord by means of metallic impregnations, immunohistochemical (glial fibrillary acidic protein) and ultrastructural methods. Three astroglial cell types have been immunohistochemically identified: ependymocytes, radial astrocytes and astrocytes. Transitional forms have also been observed. Scarce immunopositive ependymocytes were located in the dorsal and ventral regions of the ependyma. The radial astrocytic somata were located around the ependymal layer and their processes reached the subpial glia limitans. Typical astrocytes were the most abundant astroglial cell type; astrocytes located in the ventral horn showed a greater development than those of the dorsal horn. In the white matter, the astrocytes were large and their processes formed part of the subpial glia limitans; on some occasions, astrocytic cell bodies also formed part of this subpial limitans. Transitional elements between astrocytes and radial astrocytes were observed in both grey and white matter. The perivascular and subpial glia limitans were continuous and showed a strong immunoreactivity. The comparative analysis of our results in the lizard spinal cord with those in other vertebrate groups leads us to conclude that reptiles could represent the key group in the phylogenetic evolution of the astroglial cells in vertebrates.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00174628
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  • 2
    Digitale Medien
    Digitale Medien
    Similarity between the effects of suprachiasmatic nuclei lesions and of pinealectomy on gonadotropin release in ovariectomized, sulpiride-treated and melatonin-replaced rats (1993)
    Springer
    Cellular and molecular life sciences 49 (1993), S. 797-801 
    Details
    ISSN: 1420-9071
    Schlagwort(e): Pinealectomy ; suprachiasmatic nuclei lesions ; ovariectomy ; LH ; FSH ; melatonin ; sulpiride-treated rats
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract The aim of this study was to compare the effects of pineal indole treatments on LH and FSH release in pinealectomized and suprachiasmatic lesioned and ovariectomized rats rendered hyperprolactinemic by acute sulpiride treatment. pinealectomy or suprachiasmatic nuclei lesions in female rats both decreased plasma LH and FHS at 10, but not at 20 d after surgery, whereas the daily afternoon administration of melatonin effectively restored levels of both gonadotropins to control values. In ovariectomized rats, pinealectomy or suprachiasmatic nuclei lesions were ineffective in counteracting the high plasma levels of LH and FSH. However, sulpiride treatment in both pinealectomized and suprachiasmatic nuclei lesioned and castrated female rats significantly decreased the levels of LH and FSH, an effect which was counteracted by daily afternoon melatonin administration. Other pineal indoles tested, i.e., 5-hydroxy- and 5-methoxytryptophol, were ineffective in regulating gonadotropin levels. The results suggest that the pineal gland, through its hormone melatonin, can modulate gonadotropin secretion by acting on a dopamine mechanism independent of hypothalamic suprachiasmatic areas.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01923552
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  • 3
    Digitale Medien
    Digitale Medien
    Astroglial pattern in the spinal cord of the adult barbel (Barbus comiza) (1993)
    Springer
    Anatomy and embryology 187 (1993), S. 385-395 
    Details
    ISSN: 1432-0568
    Schlagwort(e): Astrocyte ; Ependymocyte ; Spinal cord Fish ; GFAP ; Vimentin
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The distribution and the structural, ultrastructural and immunohistochemical characteristics of the astroglial cells in the spinal cord of the adult barbel (Barbus comiza) have been studied by means of metallic impregnations (Golgi and gold-sublimate), immunohistochemical (GFAP and vimentin) and electron microscopic techniques. GFAP-positive cells were mainly distributed in the ependyma and in the periependymal region, but they have also been observed at subpial level in the anterior column. The ependymocytes were heterogeneous cells because they showed different immunohistochemical characteristics: GFAP-positive, vimentin-positive or non-immunoreactive cells. The radial astrocytes showed only GFAP immunoreactivity, and their processes ended at the subpial zone forming a continuous subpial glia limitans. Desmosomes and gap junctions between soniata and processes of radial astrocytes were numerous, and a relationship between radial astroglial processes and the nodes of Ranvier was also described. The perivascular glia limitans was poorly developed and it was not complete in the blood vessels of the periependymal zone; in this case, the basal lamina was highly developed. An important characteristic in the barbel spinal cord was the existence of a zone with an abundant extracellular space near the ependyma. The presence of radial astroglial somata at subpial level, the existence of vimentin-positive ependymocytes and the abundant extracellular space in the periependymal zone is discussed in relation to the regeneration capacity and the continuous growth showed by fish. Moreover, the abundance of gliofilaments and desmosomes leads us to suggest that mechanical support might be an important function for the astroglial cells in the barbel spinal cord.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00185897
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  • 4
    Digitale Medien
    Digitale Medien
    High ammonia diet: Its effect on the glial fibrillary acidic protein (GFAP) (1993)
    Springer
    Neurochemical research 18 (1993), S. 971-975 
    Details
    ISSN: 1573-6903
    Schlagwort(e): GFAP ; spinal cord ; ammonia ; hyperammonemia ; astroglia
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The effect of a recent hyperammonemic model, consisting of a high ammonia diet for 3, 7, 15, 45, and 90 days, on glial fibrillary acidic protein (GFAP) in the rat spinal cord and on blood ammonia levels has been studied. The high ammonia diet was prepared by mixing a standard diet with ammonium acetate (20% wt/wt); in addition, 5 mM of ammonium acetate was added to the water supply. GFAP contents were determined by means of immunoblotting analysis. The results demonstrated that this high ammonia diet model neither induces significant changes in GFAP immunoreactivity, nor modifies total protein concentration, and only induces significant blood hyperammonemic levels in the first days of treatment. An adaptative response to the diet is suggested and discussed to explain these results. A relation between ammonia and GFAP expression is suggested because transient hyperammonemia induces transient, although no significant, changes on GFAP expression.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00966755
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  • 5
    Digitale Medien
    Digitale Medien
    Radial astrocytes and ependymocytes in the spinal cord of the adult toad (Bufo bufo L.) (1990)
    Springer
    Cell & tissue research 260 (1990), S. 307-314 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Astroglial cells ; Ependymocytes ; Spinal cord ; Immunohistochemistry ; Bufo bufo L. (Anura)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Immunohistochemical and ultrastructural techniques have been used to demonstrate glial fibrillary acidic protein (GFAP) immuno-positive cells in the adult toad spinal cord. Two types of GFAP-immunoreactive cells were observed: ependymocytes and radial astrocytes. GFAP-positive ependymocytes were scarce and contained the immunoreactive product in their processes. They showed intermediate filaments in the basal pole and in their processes when studied with the electron microscope. These immuno-positive ependymocytes represent the tanycytic form of ependymal cells because their processes ended at the subpial zone. The radial astrocytes showed a more intensive immunoreactive product in somata and processes when they were located far away from the ependymal layer. Cell bodies and processes were also associated with blood vessels, but most of the processes ended at the subpial zone forming a continuous subpial glia limitans. The GFAP-positive processes, which form this subpial glia limitans in the toad spinal cord, belong to both tanycytic ependymocytes and radial astrocytes, whose somata are located in the grey matter. These findings lead us to suggest that both types of GFAP-immunopositive cells might be the functional equivalents of mammalian astrocytes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00318633
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