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  • 1
    Electronic Resource
    Electronic Resource
    Carboxin resistance transformation of the homobasidiomycete fungus Pleurotus ostreatus (2000)
    Springer
    Current genetics 37 (2000), S. 209-212 
    Details
    ISSN: 1432-0983
    Keywords: Key words Basidiomycete ; Transformation ; White-rot fungi ; Carboxin ; Succinate:ubiquinone oxidoreductase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A novel selection marker gene for transformation of the white-rot basidiomycete Pleurotus ostreatus was developed by introducing a point mutation in a gene which encodes the iron-sulfur protein (Ip) subunit of succinate dehydrogenase. The mutant gene, Cbx R, encodes a modified Ip subunit with an amino-acid substitution (His239 to Leu) and confers resistance to the systemic fungicide, carboxin. The DNA sequence was integrated ectopically in the chromosome of the transformants. This is the first report of a homologous marker gene which is available for the molecular breeding of an edible mushroom.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050521
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Visualization of microtubules in living cells of transgenicArabidopsis thaliana (1999)
    Springer
    Protoplasma 206 (1999), S. 201-206 
    Details
    ISSN: 1615-6102
    Keywords: Cytoskeleton ; Epidermal cell ; GFP-TUA6 fusion protein ; Microtubule ; Transgenic plants ; Tubulin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Microtubules (MTs) were visualized in living cells of several tissues in transgenicArabidopsis thaliana. The transformed Arabidopsis plant was obtained by infecting it withAgrobacterium tumefaciens carrying the GFP-TUA6 plasmid. The fluorescence of the MTs was due to the fluorescence of GFP-TUA6 that was polymerized into the MTs. The distribution patterns of the visualized MTs in the living epidermal cells of leaves was similar to that in fixed epidermal cells. The actual destruction of MTs by oryzalin was observed in a living cell. Cytochalasin B exerts no effect on the distribution pattern of MTs. The fluorescence intensity of MTs was different among cells in different tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279267
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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