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Digitale Medien

In vivo cloning of DNA into multicopy cosmids by mini-Mu-cosduction (1986)

Mendoza, Diego ; Gramajo, Hugo C. ; Rosa, Alberto L.

Springer

Molecular genetics and genomics 205 (1986), S. 546-549

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Details

ISSN: 1617-4623

Schlagwort(e): Transposition ; Genomic libraries ; Recombinant DNA

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Summary A general in vivo procedure for cloning Escherichia coli genes into cosmids has been developed. The method we describe here uses a deleted Mu phage (a mini-Mu) to transpose E. coli genes into cosmids during mini-Mu replication. The resulting cosmids clones are packaged in-vivo into λ phage particles. Plasmids carrying a particular DNA sequence can be selectively recovered after infection of a new host with the in vivo constructed genomic cosmid library. This system was used succesfully to clone several E. coli genes.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00338096

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