ISSN:
1432-2307
Keywords:
Indirect immunoperoxidase staining
;
Tissue section
;
Fungi
;
Immunohistochemistry
;
Polyclonal antibody
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract This study was performed to identify pathological fungi of eight species [Aspergillus fumigatus, Candida albicans, Torulopsis (Candida) glabrata, Cryptococcus neoformans, Fusarium anthophilum, Rhizopus oryzae, Sporothrix schenckii and Trichosporon beigelii] in formalin-fixed, paraffin-embedded tissue sections by indirect immunoperoxidase staining. Mature albino rabbits were immunized with formalin-killed organisms. Antibodies were prepared by precipitation. Immunoperoxidase staining was applied to the paraffin-embedded tissue sections of experimentally infected mice and human autopsy and surgical specimens. Although the cell walls of each fungus stained clearly, many cross-reactivities appeared. However, it was possible to obtain specificity for the eight species by absorption and dilution of the antisera.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00199390
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