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  • 1995-1999  (2)
  • Human heart  (1)
  • Key words thermal modeling  (1)
  • 1
    ISSN: 1432-1440
    Keywords: L-type calcium channel ; β Subunit isoforms ; Human heart ; Cardiac hypertrophy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We investigated the expression of α1 and β subunits of the L-type Ca2+ channel on the protein level in cardiac preparations from normal human heart ventricles and from the hypertrophied septum of patients with hypertrophic obstructive cardiomyopathy (HOCM). 1,4-Dihydropyridine (DHP) binding and immunorecognition by polyclonal antibodies directed against the C-terminal amino acid sequences of the β2 and β3 subunits were used for detection and quantification of α1, β2, and β3 subunits. Bmax of high-affinity DHP binding was 35±2 fmol/mg protein in HOCM and 20±2 fmol/mg protein in normal human hearts (P〈0.05). In rabbit hearts the anti-β2 subunit antibody immunoprecipitated 80% of the total amount of DHP-labeled Ca2+ channels present in the assay. Under identical experimental conditions 25% of labeled Ca2+ channels were recovered in the immunoprecipitates of both normal and HOCM ventricles. A similar partial immunoprecipitation was observed in pig hearts. Immunoblot analysis demonstrated that the β2 subunit was associated with the DHP receptor/Ca2+ channel in cardiac muscle of rabbit, pig, and human heart. In neither of these purified cardiac Ca2+ channels was the β3 subunit isoform detected. Our results suggest that both α1 and β2 subunit expression is upregulated in HOCM in a coordinate manner.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Geologische Rundschau 86 (1997), S. S87 
    ISSN: 0016-7835
    Keywords: Key words thermal modeling ; intrusion depth ; melt temperature ; KTB ; Bohemian Massif ; variscides ; Falkenberg granite
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract  The Late Carboniferous Falkenberg granite, exposed 2 km to the east of the German Continental Deep Drilling (KTB) drill site, has solidified at a depth of approximately 9–12 km. The initial temperature of the intrusion was 780–800 °C. The shape of the pluton is approximately that of a horizontal plate with an assumed original thickness of approximately 9 km, 3 km of which having now been removed by erosion. The results of simple one-dimensional thermal modeling, based on conductive heat transfer, suggest cooling to 400 and 350 °C over approximately 6 and 15 m.y., respectively. With respect to the cooling ages of micas, this suggests that the intrusion is somewhat older than previously assumed. The lack of thermal influence in the nearby crustal section recovered by KTB, compared with the width of the contact aureole inferred from the model, is explained by considerable convergence between contact and drilling site. The initial (synemplacement) distance between the granite/ wall-rock contact and the KTB location was at least twice the recent value. This is consistent with Mesozoic crustal shortening, which has resulted in the antiformal stack geometry of the supracrustal slices drilled by KTB.
    Type of Medium: Electronic Resource
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