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  • Artikel: DFG Deutsche Nationallizenzen  (18)
  • Analytical Chemistry and Spectroscopy  (11)
  • Physical Chemistry  (4)
  • Gene expression  (3)
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  • Artikel: DFG Deutsche Nationallizenzen  (18)
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  • 1
    Digitale Medien
    Digitale Medien
    Plasma-membrane H+-ATPase gene expression is regulated by NaCl in cells of the halophyte Atriplex nummularia L. (1993)
    Springer
    Planta 190 (1993), S. 433-438 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Atriplex ; Gene expression ; NaCl regulation ; Halophyte ; Plasma-membrane H+-ATPase
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract An Atriplex nummularia L. cDNA probe encoding the partial sequence of an isoform of the plasma-membrane H+ -ATPase was isolated, and used to characterize the NaCl regulation of mRNA accumulation in cultured cells of this halophyte. The peptide (447 amino acids) translated from the open reading frame has the highest sequence homology to the Nicotiana plumbaginifolia plasma-membrane H+-ATPase isoform pma4 (greater than 80% identity) and detected a transcript of approximately 3.7 kb on Northern blots of both total and poly(A)+ RNA. The mRNA levels were comparable in unadapted cells, adapted cells (cells adapted to and growing in 342 mM NaCl) and deadapted cells (cells previously adapted to 342 mM NaCl that are now growing without salt). Increased mRNA abundance was detected in deadapted cells within 24 h after exposure to NaCl but not in unadapted cells with similar salt treatments. The NaCl up-regulation of message abundance in deadapted cells was subject to developmental control. Analogous to those reported for glycophytes, the plasma-membrane H+-ATPase are encoded by a multigene family in the halophyte.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00224780
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  • 2
    Digitale Medien
    Digitale Medien
    Induction of cardiac angiotensin I-converting enzyme with dietary NaCl-loading in genetically hypertensive and normotensive rats (1995)
    Springer
    Journal of molecular medicine 73 (1995), S. 243-248 
    Details
    ISSN: 1432-1440
    Schlagwort(e): Angiotensin I-converting enzyme ; Gene expression ; Sodium chloride ; Heart ; Inbred rats
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract We have recently shown that the angiotensin I converting enzyme (ACE) gene is linked to NaCl-loaded blood pressure in the stroke-prone spontaneously hypertensive rat (SHRSP), and that high-NaCl loading selectively stimulates ACE in the aorta of SHRSP but not in normotensive Wistar-Kyoto (WKY) rats. We therefore investigated the relationship between cardiac ACE and the development of hypertension and left ventricular hypertrophy in response to normal- and high-NaCl diet in these rats. ACE mRNA and ACE activity were measured in left ventricular tissue after completion of hemodynamic characterization of the animals. While SHRSP rats increased blood pressure (P〈0.0001) and heart rate (P〈0.005) in response to high NaCl, blood pressure remained unchanged in WKY. Similarly, relative left ventricular weight increased only in SHRSP after high NaCl (P〈0.002). A significant two- to threefold increase of cardiac ACE mRNA and fourfold stimulation of ACE enzyme activity in response to high NaCl was found in both WKY and SHRSP rats (P〈0.005). The induction of ACE gene expression was significantly more pronounced in SHRSP compared to WKY (P〈0.02), whereas no significant strain differences in left ventricular ACE activity were found after either normal- or high-NaCl diet. Thus, arterial blood pressure and left ventricular weight remained unchanged in the WKY rats despite the activation of left ventricular ACE activity after high-NaCl exposure. These results demonstrate that left ventricular ACE activity is equally upregulated in response to high-NaCl in the normotensive and hypertensive strain, independently from the development of hypertension. We conclude that the pretranslational induction of left ventricular ACE with high-NaCl loading may be important both for the regulation of cardiac angiotensins and kinins and for local therapeutic ACE inhibition in the heart during high-salt status.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00189924
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  • 3
    Digitale Medien
    Digitale Medien
    Cold stress response in Archaea (2000)
    Springer
    Extremophiles 4 (2000), S. 321-331 
    Details
    ISSN: 1433-4909
    Schlagwort(e): Key words Cold shock ; Low-temperature adaptation ; Psychrophile ; Adaptive mechanisms ; Antarctic Archaea ; Gene expression ; Protein structure ; Review
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We live on a cold planet where more than 80% of the biosphere is permanently below 5°C, and yet comparatively little is known about the genetics and physiology of the microorganisms inhabiting these environments. Based on molecular probe and sequencing studies, it is clear that Archaea are numerically abundant in diverse low-temperature environments throughout the globe. In addition, non-low-temperature-adapted Archaea are commonly exposed to sudden decreases in temperature, as are other microorganisms, animals, and plants. Considering their ubiquity in nature, it is perhaps surprising to find that there is such a lack of knowledge regarding low-temperature adaptation mechanisms in Archaea, particularly in comparison to what is known about archaeal thermophiles and hyperthermophiles and responses to heat shock. This review covers what is presently known about adaptation to cold shock and growth at low temperature, with a particular focus on Antarctic Archaea. The review highlights the similarities and differences that exist between Archaea and Bacteria and eukaryotes, and addresses the potentially important role that protein synthesis plays in adaptation to the cold. By reviewing the present state of the field, a number of important areas for future research are identified.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s007920070001
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  • 4
    Digitale Medien
    Digitale Medien
    Automated probe mass spectrometry (1989)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 18 (1989), S. 733-737 
    Details
    ISSN: 0887-6134
    Schlagwort(e): Chemistry ; Analytical Chemistry and Spectroscopy
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: A method of fully automating the analysis of routine samples using probe introduction techniques has been designed and developed. Unattended 24 h operation has dramatically increased the ability to process large numbers of samples and has freed much of an operator's time to perform other tasks.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bms.1200180915
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  • 5
    Digitale Medien
    Digitale Medien
    Short Communications. Biphenyl-3,4′-Dinitrene (1991)
    Chichester : Wiley-Blackwell
    Journal of Physical Organic Chemistry 4 (1991), S. 459-462 
    Details
    ISSN: 0894-3230
    Schlagwort(e): Organic Chemistry ; Physical Chemistry
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Physik
    Notizen: Photolysis of biphenyl-3,4′ -diazide in a rigid glassy matrix at 77 K yields quintet state biphenyl-3,4′ -dinitrene with zero-field splitting parameters of |D/hc- = 0.153 cm-1 and |E/hc| = 0.019 cm-1 determined by electron spin resonance spectroscopic studies. Curie plot studies are consistent with assigning the quintet to be the ground state in this species. This finding confirms qualitative connectivity-based predictions for this general connectivity type of openshell system, and is in qualitative agreement with spectral INDO-CI computational predictions for both planar and twisted geometries of the dinitrene.
    Zusätzliches Material: 2 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/poc.610040708
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  • 6
    Digitale Medien
    Digitale Medien
    Zero-field splitting versus interelectronic distance in triplet electron spin resonance spectra of localized dinitrenes (1993)
    Chichester : Wiley-Blackwell
    Journal of Physical Organic Chemistry 6 (1993), S. 483-487 
    Details
    ISSN: 0894-3230
    Schlagwort(e): Organic Chemistry ; Physical Chemistry
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Physik
    Notizen: Triplet zero-field splitting parameters were obtained in 2-methyltetrahydrofuran glass at 77 K for phenylene-1,4-dinitrene (1), biphenyl-4,4′-dinitrene (2), (E)-1,2-bis(4′-nitrenophenyl)ethene (3), 1,4-bis(4′-nitrenophenyl)buta-1,3-diene (4) and 1,8-bis(4′-nitrenophenyl)octa-1,3,5,7-tetraene (5). The results were (1) |D/hc| = 0·169 cm-1 |E/hc| = 0·004 cm-1, (2) |D/hc| = 0·189 cm-1, |E/hc| = 0·00 cm-1, (3) |D/hc| = 0·122 cm-1, |E/hc| = 0·00 cm-1, (4) |D/hc| = 0·0865 cm-1, |E/hc| = 0·00 cm-1 and (5) |D/hc| = 0·0442 cm-1, |E/hc| = 0·00 cm-1. All these biradicals are ground-state singlets. Based on the observed decrease in triplet signal intensities as temperature decreases. The substantial magnitudes of |D/hc| for 3-5, despite the large distance between localized nitrene electrons, is much more than can be explained by a simple dipolar interaction between localized electrons, and is attributed at least partly to spin polarization effects on the π-electron clouds of these systems.
    Zusätzliches Material: 1 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/poc.610060807
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  • 7
    Digitale Medien
    Digitale Medien
    Intramolecular exchange coupling of arylnitrenes by OXYGEN (1994)
    Chichester : Wiley-Blackwell
    Journal of Physical Organic Chemistry 7 (1994), S. 495-502 
    Details
    ISSN: 0894-3230
    Schlagwort(e): Organic Chemistry ; Physical Chemistry
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Physik
    Notizen: A series of m,n′-diazidodiphenyl ethers (m ≤ n, m = 3,4; n = 3,4) was photolyzed at 77 K in frozen, glassy 2-methyltetrahydrofuran matrices to generate the corresponding diphenyl ether m,n′-dinitrenes for study by electron spin resonance (ESR) spectroscopy. 3,4′-Diazidodiphenyl ether gave an ESR spectrum dominated by a mononitrene peak with ∣D/hc∣ = 0·972 cm-1, and also showed a weak dinitrene quintet spectrum with ∣D/hc∣ = 0·162 cm-1 having ESR spectral intensity vs temperature dependence (Curie law) consistent with either a high-spin ground state or a very small singlet-quintet gap. Di(3-azidophenyl) ether gave a strong mononitrene peak with ∣D/hc∣ = 0·996 cm-1 and a quintet dinitrene ESR spectrum (∣D/hc∣ = 0·162 cm-1) which exhibited non-linear Curie law intensity behavior consistent with the quintet being a thermally populated excited state 40 cal mol-1 above a singlet ground state. Di(4-azidophenyl) ether gave a strong mononitrene peak with ∣D/hc∣ = 0·961 cm-1, but no observable spectrum related to a high-spin open-shell dinitrene. The results are consistent with oxygen being a weak exchange coupling linker in pi-conjugated open-shell molecules. The observed ground-state spin multiplicities are in accord with qualitative superexchange and connectivity models, despite any perturbations due to resonance effects between the oxygen linker and p-nitrene sites.
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/poc.610070905
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  • 8
    Digitale Medien
    Digitale Medien
    Enthalpies of micelle formation by hexadecyltrimethylammonium bromide in aqueous solution containing pentanol (1995)
    Chichester : Wiley-Blackwell
    Journal of Physical Organic Chemistry 8 (1995), S. 108-112 
    Details
    ISSN: 0894-3230
    Schlagwort(e): Organic Chemistry ; Physical Chemistry
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Physik
    Notizen: Titration calorimetric data show a dramatic change from endo- to exothermic deaggregation when pentanol-hexadecyltrimethylammonium bromide (CTAB) mixed solutions are injected into an aqueous solution containing pentanol. The results are interpreted in terms of a change in the structures of the aggregates in solution from simple CTAB micelles to mixed amphiphilic microheterogeneities when pentanol is added.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/poc.610080210
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  • 9
    Digitale Medien
    Digitale Medien
    Measurement of (C13)arginine incorporation into apolipoprotein B-100 in very low density lipoproteins and low density lipoproteins in normal subjects using (13C)sodium bicarbonate infusion and isotope ratio mass spectrometry (1990)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 19 (1990), S. 459-464 
    Details
    ISSN: 1052-9306
    Schlagwort(e): Chemistry ; Analytical Chemistry and Spectroscopy
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: We describe a new stable isotope technique for the in vivo study of hepatic plasma protein synthesis in humans. The method involves the infusion of (13C)sodium bicarbonate for 1 h and the measurement of the isotopic enrichment of (13C)arginine in newly synthesized apolipoprotein B of very low density lipoproteins (VLDL-apoB) and low density lipoproteins (LDL-apoB) in blood samples taken over a 5-6 h period from the commencement of the infusion. Isotope ratio mass spectrometry was utilized to measure 13CO2 enrichment following hydrolysis of these proteins and conversion of the guanidinium carbon of arginine in the hydrolysate to carbon dioxide by sequential incubation with arginase and urease. The method is capable of measuring isotopic enrichment as low as 0.001 at. % excess (APE) with a precision of 1.2%. In both subjects studied, the (13C)arginine of VLDL-apoB reached enrichments of 0.2 APE and that of the arginine of LDL-apoB, 0.03 APE. Incorporation of labeled arginine into LDL-apoB was demonstrable at 60-90 min. The new technique is safe and is applicable to the study of the hepatic biosynthesis of a wide range of plasma proteins.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bms.1200190803
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  • 10
    Digitale Medien
    Digitale Medien
    Routine clinical determination of carotene, vitamin E, vitamin A, 25-hydroxy vitamin D3 and trans-vitamin K1 in human serum by straight phase HPLC (1987)
    New York, NY : Wiley-Blackwell
    Biomedical Chromatography 2 (1987), S. 79-88 
    Details
    ISSN: 0269-3879
    Schlagwort(e): Chemistry ; Analytical Chemistry and Spectroscopy
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Notizen: A universal extraction procedure is described for fat-soluble vitamins in human serum. Methods are presented for routine quantitative analysis by isocratic straight phase HPLC with UV-detection of (α + β)-carotene, vitamin E. (α-tocopherol) and vitamin A (all-trans-retinol) in one single run, and of vitamin K1 (trans-phylloquinone) and 25-hydroxy vitamin D3 after sample clean-up using disposable reversed-phase cartridges. The limits of detection, precisions and selectivities of the developed assays are shown to be satisfactory after more than three years' experience. The routine clinical determination of fat-soluble vitamins can be performed in less than 5 mL of serum. Analyses of external quality control and randomly taken outpatient samples are shown to be of great value in assessing laboratory performance.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bmc.1130020209
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