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  • 1
    Electronic Resource
    Electronic Resource
    Wash out kinetics of Na from the transport pool to the epithelial and corium side of the frog skin (1972)
    Springer
    Pflügers Archiv 337 (1972), S. 285-297 
    Details
    ISSN: 1432-2013
    Keywords: Frog Skin ; Na Transport ; Transport Pool ; Tracer Wash Out ; Ouabain
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The wash out kinetics of Na-24 out of the transport pool of the frog skin were followed simultaneously to the epithelial and corium side in control experiments and after inhibition of the Na transport with ouabain. A perfusion system was used which allowed changes of the perfusion chambers within 5 sec between labelling and tracer wash out. In the control, the Na of the transport pool is washed out with a half time of 2.9±0.3 min and 3.2±0.7 min to the corium and the epithelial side, respectively. After ouabain, the half times increased to 8.9±0.9 min and 8.6±1.1 min for the corium and the epithelial wash out. From this observation it is concluded that the transport Na is distributed in an intracellular compartment. The ratio of the two tracer effluxes from the transport pool to the corium and the epithelial sidej 23 * /j 21 * is 30±8 in the control and 4.9±2.0 after ouabain. The wash out to the epithelial side shows an additional fast component (half times 0.70±0.08 min in the control and 0.68±0.13 after ouabain) which is interpreted as wash out of an extracellular compartment. This view is supported by measurements of the wash out of Inulin C-14.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00586646
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Analysis of sodium compartments and fluxes in frog skin (1974)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 281 (1974), S. 281-294 
    Details
    ISSN: 1432-1912
    Keywords: Sodium Transport ; Frog Skin ; Compartment Analysis ; Transport Pool
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The path of the transepithelial Na transport through the frog skin was investigated by the use of compartment analysis. Therefore tracer wash-out kinetics from the preloaded epithelium to both sides of the skin and time course of tracer uptake at the epithelial side were measured under control conditions and after alteration of transport parameter by amiloride, ouabain and incubation at 0°C. In all experiments the skins were short-circuited throughout and the SCC registered. The results indicate that the major part of the Na involved in transport is already transported and located in an extracellular compartment, possibly the intercellular space between the epithelial cells. The decisive step of the transepithelial transport seems to be located at the outer epithelial border of the cell layer since entrance of Na into the skin is similarly altered by all inhibitors of transport. Two additional compartments which are labelled from the epithelial side are detectable. The Na content of the compartment with the lowest exchange rate is increased by ouabain and 0° C but remains unchanged after amiloride. It might be located intracellularly but is not responsible for transepithelial transport. The actual transport pool must be very small.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00500597
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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