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  • 1
    Digitale Medien
    Digitale Medien
    Control of Carbon and Nitrogen Metabolism in Bacillus Subtilis (1991)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Microbiology 45 (1991), S. 107-135 
    Details
    ISSN: 0066-4227
    Quelle: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Thema: Biologie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1146/annurev.mi.45.100191.000543
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  • 2
    Digitale Medien
    Digitale Medien
    A Bacillus subtilis dipeptide transport system expressed early during sporulation (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 5 (1991), S. 0 
    Details
    ISSN: 1365-2958
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie , Medizin
    Notizen: Two previously identified Bacillus subtilis DNA segments, dciA and dciB, whose transcripts accumulate very rapidly after induction of sporulation, were found in the same 6.2 kb transcription unit, now known as the dciA operon. Analysis of the sequence of the dciA operon showed that its putative products are homologous to bacterial peptide transport systems. The product of the fifth gene, DciAE, is similar to peptide-binding proteins from Escherichia coli and Salmonella typhimurium (DppA and OppA) and B. subtilis (OppA). A null mutation in dciAE abolished the ability of a proline auxotroph to grow in a medium containing the dipeptide Pro–Gly as sole proline source, suggesting that the dciA operon encodes a dipeptide transport system.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2958.1991.tb00814.x
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  • 3
    Digitale Medien
    Digitale Medien
    Transcriptional regulation of a Bacillus subtilis dipeptide transport operon (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 5 (1991), S. 0 
    Details
    ISSN: 1365-2958
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie , Medizin
    Notizen: The Bacillus subtilis dciA operon, which encodes a dipeptide transport system, was induced rapidly by several conditions that caused the cells to enter stationary phase and initiate sporulation. The in vivo start point of transcription was mapped precisely and shown to correspond to a site of transcription initiation in vitro by the major vegetative form of RNA polymerase. Post-exponential expression was prevented by a mutation in the spo0A gene (whose product is a known regulator of early sporulation genes) but was restored in a spo0A abrB double mutant. This implicated AbrB, another known regulator, as a repressor of dciA. In fact, purified AbrB protein bound to a portion of the dciA promoter region, protecting it against DNase I digestion. Expression of dciA in growing cells was also repressed independently by glucose and by a mixture of amino acids; neither of these effects was mediated by AbrB.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2958.1991.tb00815.x
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  • 4
    Digitale Medien
    Digitale Medien
    Identification of Bacillus subtilis genes expressed early during sporulation (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 3 (1989), S. 0 
    Details
    ISSN: 1365-2958
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie , Medizin
    Notizen: Labelled cDNA transcribed in vitro from early-sporulation RNA was enriched for sporulation-specific sequences by subtractive hybridization to an excess of vegetative RNA and used to probe libraries of Bacillus subtilis chromosomal DNA. From the initial collection of clones that coded for RNAs transcribed preferentially during sporutation, several were subcloned and studied in more detail. It was found that two clones contained sequences (dciA and dciB) that had an undetectable level of transcription during vegetative growth but had transcripts that started to appear no later than eight minutes after induction of sporulation. A third DNA segment (dciC) was expressed at a low level in vegetative cells and increased within four minutes after induction of sporulation.The effects of spo0 mutations, i.e. mutations that prevent cells from reaching stage I of the sporulation process, were tested. Induction of the dciA and dciB transcripts was significantly reduced in strains carrying mutations in the spo0A and spo0H genes but not in a spo0B mutant strain. In addition, a product of the abrB locus, a locus in which mutations are known to partially overcome the pleiotropic effect of spo0A and spo0B mutations, seemed to be required for dciA and dciB expression.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2958.1989.tb00257.x
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  • 5
    Digitale Medien
    Digitale Medien
    Nucleotide sequences that signal the initiation of transcription and translation inBacillus subtilis (1982)
    Springer
    Molecular genetics and genomics 186 (1982), S. 339-346 
    Details
    ISSN: 1617-4623
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary We have determined the nucleotide sequence of twoBacillus subtilis promoters (veg andtms) that are utilized by the principal form ofB. subtilis RNA polymerase found in vegetative cells (σ55-RNA polymerase) and have compared our sequences to those of several previously reportedBacillus promoters. Hexanucleotide sequences centered approximately 35 (the “-35” region) and 10 (the “-10” region) base pairs upstream from theveg andtms transcription startpoints (and separated by 17 base pairs) corresponded closely to the consensus hexanucleotides (TTGACA and TATAAT) attributed toEscherichia coli promoters. Conformity to the preferred -35 and -10 sequences may not be sufficient to promote efficient utilization byB. subtilis RNA polymerase, however, since three promoters (veg, tms andE. coli tac) that conform to these sequences and that are utilized efficiently byE. coli RNA polymerase were used with highly varied efficiencies byB. subtilis RNA polymerase. We have also analyzed mRNA sequences in DNA located downstream from eightB. subtilis chromosomal and phage promoters for nucleotide sequences that might signal the initiation of translation. In accordance with the rules of McLaughlin, Murray and Rabinowitz (1981), we observe mRNA nucleotide sequences with extensive complementarity to the 3′ terminal region ofB. subtilis 16S rRNA, followed by an initiation codon and an open reading frame.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00729452
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