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  • 1
    Digitale Medien
    Digitale Medien
    Formation and stability of interstrand cross-links induced by cis- and trans-diamminedichloroplatinum (II) in the DNA of Saccharomyces cerevisiae strains differing in repair capacity (1989)
    Springer
    Current genetics 16 (1989), S. 331-338 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Platinum compounds ; Yeast ; Repair mutants ; Interstrand cross-links ; DNA degradation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Four haploid yeast strains differing in proficiency for DNA repair were treated with cis- or transDDP. The wild type was least sensitive while the excision-deficient mutants rad1, rad2 and snm1exhibited higher sensitivities to either platinum compound. In all four strains tested cisDDP showed a two- to five-fold higher cytotoxicity than equimolar concentrations of transDDP. DNA interstrand cross-linking was caused by both agents in all strains. However, transDDP introduced more DNA cross-links at exposure times up to 6 h while cisDDP was the more active cross-linking agent at longer times. There was no clear-cut correlation of the number of DNA interstrand cross-links with survival. Formaldehyde-treated cells showed DNA with lower buoyant density due to proteinase K sensitive DNA-protein cross-linking; this effect was not observed after treatment with either platinum compound. Post-treatment incubation of wild-type cells exposed to cisDDP led to degradation of DNA by single and double-strand breaks, parallel with further increase of DNA interstrand cross-linking. DNA from transDDP-treated cells did not show extensive degradation although interstrand cross-links were lost during liquid holding.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00340711
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  • 2
    Digitale Medien
    Digitale Medien
    A recessive mutant allele of the HNM1 gene of Saccharomyces cerevisiae is responsible for hyper-resistance to nitrogen mustard (1990)
    Springer
    Current genetics 18 (1990), S. 187-192 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Mutagen hyper-resistance ; Nitrogen mustard ; Saccharomyces cerevisiae
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A screening of haploid yeast strains for enhanced resistance to nitrogen mustard (HN2) yielded a recessive mutant allele, hnm1, that conferred hyper-resistance (HYR) to HN2. Diploids, homo- or heterozygous for the HNM1 locus, exhibit normal wild-type like resistance while homozygosity for hnm1 leads to the phenotype HYR to HN2. The hnm1 mutation could be found in yeast strains proficient or deficient in different DNA repair systems. In these mostly HN2-sensitive haploid repair-deficient mutants, hnm1 acted as a partial suppressor of HN2 sensitivity. All isolated recessive mutations conferring hyper-resistance belonged to a single complementations group. The HYR to HN2 phenotype was maximally expressed in growing cells and was associated with reduced mutability by HN2. HNM1 most probably controls uptake of HN2 which would be impaired in the hnm1 mutants.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00318378
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  • 3
    Digitale Medien
    Digitale Medien
    The role of PSO and SNM genes in DNA repair of the yeast Saccharomyces cerevisiae (1990)
    Springer
    Current genetics 18 (1990), S. 387-393 
    Details
    ISSN: 1432-0983
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00309906
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  • 4
    Digitale Medien
    Digitale Medien
    Isolation and characterization of additional genes influencing resistance to various mutagens in the yeast Saccharomyces cerevisiae (1992)
    Springer
    Current genetics 21 (1992), S. 319-324 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Multi-copy plasmid ; Hyper-resistance ; 4-NQO ; MNNG ; Triaziquone
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Screening of a multi-copy vector-based yeast genomic library in haploid cells of wild-type Saccharomyces cerevisiae yielded transformants hyper-resistant to various chemical mutagens. Genetical analysis of the yeast insert DNAs revealed three genes SNG1, SNQ2, and SNQ3 that confer the phenotype hyper-resistance to MNNG, to 4-NQO and triaziquone, and to mutagens 4-NQO, MNNG, and triaziquone, respectively. Integration of the gene disruption-constructs into the haploid yeast genome yielded viable null-mutants with a mutagen-sensitive phenotype. Thus, copy number of these non-essential yeast genes determines the relative resistance to certain chemical mutagens, with zero copies yielding a phenotype of mutagen sensitivity and multiple copies one of mutagen hyper-resistance, respectively.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00351689
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  • 5
    Digitale Medien
    Digitale Medien
    Overexpression of the SNQ3/YAP1 gene confers hyper-resistance to nitrosoguanidine in Saccharomyces cerevisiae via a glutathione-independent mechanism (1994)
    Springer
    Current genetics 25 (1994), S. 469-471 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Yeast ; GSH ; DNA alkylation ; MNNG
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The MNNG hyper-resistance of yeast transformants containing multiple copies of the SNQ3/YAP1 yeast gene is not caused by lowered MNNG activation due to depleted pools of glutathione. On the contrary, the SNQ3/YAP1-encoded protein stimulates production of GSH, apparently by promoter activation due to the AP-1 recognition element. Expression of at least one further gene, encoding a protein with a strong detoxifying activity, must also be stimulated to explain the MNNG hyper-resistance phenotype.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00351788
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  • 6
    Digitale Medien
    Digitale Medien
    Interactions among genes controlling sensitivity to radiation (RAD) and to alkylation by nitrogen mustard (SNM) in yeast (1982)
    Springer
    Current genetics 5 (1982), S. 33-38 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Saccharomyces cerevisiae ; Multiple mutants of DNA repair ; Sensitivity to nitrogen mustard and to radiation ; Thermoconditional DNA repair
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Three haploid yeast mutants (snm) sensitive or thermoconditionally sensitive to the DNA cross-linking agent nitrogen mustard (HN2) were crossed with four rad strains representing mutations in the three pathways of DNA dark repair. The resulting haploid double and triple mutant strains were tested for their sensitivity to UV, HN2 and HN1. From the observed epistatic or synergistic interactions of the combinations of mutant alleles we could derive the relation of the SNM1 and SNM2 genes to the postulated repair pathways. Alleles snm1-1 and snml-2 ts were found epistatic to genes of the rad3 group, whereas snm2-1 ts was epistatic to rad6. The snm1 and snm2 mutant alleles interacted synergistically. From these data it is concluded that the SNM1 gene product plays a cross-link specific role in excision repair while the SNM2 gene product may be involved in a system of error-prone repair.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00445738
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  • 7
    Digitale Medien
    Digitale Medien
    Mutant Gene snm2-1 ts, conferring thermoconditional mutagen sensitivity in Saccharomyces cerevisiae, is allelic with RAD5 (1982)
    Springer
    Current genetics 5 (1982), S. 93-95 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Yeast ; Thermoconditional DNA repair ; Mutagenesis ; Allelism test
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Of two mutant genes (snm1-2 ts and snm2-1 ts) conferring thermoconditional mutagen sensitivity in Saccharomyces cerevisiae one (snm2-1 ts) is shown to be centromere-linked. At the restrictive temperature this allele reduces UV-induced back mutation frequency of the ochre allele hiss-2 but has no influence on forward mutation at the CAN1 locus. Complementation tests and recombination analysis revealed snm2 ts to be allelic with rad5 (rev2).
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00365699
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  • 8
    Digitale Medien
    Digitale Medien
    Isolation and characterization of yeast mutants with thermoconditional sensitivity to the bifunctional alkylating agent nitrogen mustard (1981)
    Springer
    Current genetics 4 (1981), S. 145-149 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Yeast mutants ; Nitrogen mustard ; Thermoconditional DNA repair
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Selection of mutants of Saccharomyces cerevisiae sensitive to the DNA cross-linking agent nitrogen mustard (HN2) at two temperatures (23 °C and 36 °C) yielded two isolates with thermoconditionally enhanced (ts) sensitivity to the mutagen. Both were due to single recessive nuclear genes. Mutant allele snm1–2 ts showed mainly ts-sensitivity to HN2, whereas mutant allele snm2-1 ts conferred ts-sensitivity to HN2, half mustard (HN1) and UV. In temperature-shift experiments it was determined that the functions of SNM1 and SNM2 are needed for recovery within 6 to 7 h. after mutagen exposure during incubation at 23 °C on YEPD when HN2 and UV are applied. After HN1 treatment the SNM2 coded function is required for recovery for about 14 hrs. This possibly indicates a handling of UV- and HN2-induced lesions different from that of HN1-induced lesions.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00365693
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  • 9
    Digitale Medien
    Digitale Medien
    Hyperresistance to formaldehyde of Saccharomyces cerevisiae seems not to be correlated with the formation and removal of DNA protein cross-links (1988)
    Springer
    Current genetics 13 (1988), S. 125-128 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Formaldehyde ; DNA-protein cross-links ; Repair ; Saccharomyces cerevisiae ; Hyperresistance
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The formation and removal of formaldehyde-mediated DNA protein cross-linking was measured by CsCI density gradient analysis in yeast strains of differing resistance to formaldehyde. Wild-type cells and transformants made hyperresistant to formaldehyde by a multi-copy vector containing the yeast SFA gene were specifically labeled in their DNA and incubated in the presence of formaldehyde. Treatment with formaldehyde lead to the formation of equal amounts of DNA protein cross-links; subsequent liquid holding of cells for 24 h resulted in the removal of nearly all DNA protein crosslinks regardless of the original formaldehyde resistance status of the strains.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00365646
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  • 10
    Digitale Medien
    Digitale Medien
    The DNA repair gene PSO3 of Saccharomyces cerevisiae belongs to the RAD3 epistasis group (1992)
    Springer
    Current genetics 21 (1992), S. 85-90 
    Details
    ISSN: 1432-0983
    Schlagwort(e): pso and rad mutants ; Repair ; S. cerevisiae ; 8-methoxypsoralen ; 3-carbethoxypsoralen
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The mutant allele pso3-1 of Saccharomyces cerevisiae confers sensitivity to treatment with UV365nm (UVA) light-activated mono- and bi-functional psoralens. When pso3-1 is combined in double mutants with selected rad and pso mutant alleles and subjected to 8-MOP+UVA treatment, epistatic interaction with regard to survival is observed with pso1, pso2, and rad3. With the same treatment the combination of pso3-1 with rad6 and rad52 leads to synergistic interaction. For the monofunctional agent 3-carbethoxypsoralen (3-CPs) the analysis of double mutants yields the same results as with the bifunctional 8-methoxypsoralen (8-MOP) with the exception of the pso1-1pso3-1 double mutant. Here we find an additive interaction, i.e., the sensitivities of both parental strains are summed in the double mutant, which indicates a different substrate specificity of the repair activity encoded by the PSO1 and PSO3 genes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00318660
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