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  • Articles: DFG German National Licenses  (14)
Source
  • Articles: DFG German National Licenses  (14)
Material
Years
  • 11
    Electronic Resource
    Electronic Resource
    Getah virus inAedes vexans nipponii andCulex tritaeniorhynchus: Vector susceptibility and ability to transmit (1983)
    Springer
    Archives of virology 76 (1983), S. 299-305 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Vector competences ofAedes (Ae.) vexans nipponii (nip.) andCulex (Cx.) tritaeniorhynchus to Getah virus were assessed by using a membrane feeding technique. The Getah virus was present at high titer in both species of mosquitoes after 21 days of extrinsic incubation at 28° C. Infection rates on 21 post-feeding were 100 per cent (4/4) forAe. vexans nip. at a virus dosage of 105.3 PFU/ml and 60 per cent (3/5) forCx. tritaeniorhynchus at similar virus dosage. More than 103.5 PFU of virus was detected in salivary glands of both species of mosquitoes on day 21 of extrinsic incubation. Forty percent (2/5) ofAe. vexans nip. transmitted the virus into serum-agar after ingesting 104.3 PFU/ml of virus blood mixture. In experiments withCx. tritaeniorhynchus ingesting 107.5 PFU/ml of virus blood mixture, 57 per cent (4/7) were able to transmit the virus to suckling mice and 59 per cent (10/17) transmitted the virus into serum-agar.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01311197
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    Paper (German National Licenses)
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  • 12
    Electronic Resource
    Electronic Resource
    In vitro antiviral activity of lactoferrin and ribavirin upon hantavirus (2000)
    Springer
    Archives of virology 145 (2000), S. 1571-1582 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary.  Bovine lactoferrin (LF) and ribavirin (Rbv) were tested as antiviral agents against Seoul type hantavirus (SR-11 strain) in vitro. Hantaviral foci number in Vero E6 cells infected with SR-11 was reduced with LF treatment by 5 days post infection to obtain a 50% effective dose (ED50) of 2500 μg/ml, while pretreatment with LF was highly efficacious having an ED50 of 39 μg/ml. Conversely, 1 h pretreatment with Rbv revealed no inhibition of viral focus formation but could significantly reduce the number of viral foci (ED50: 10 μg/ml) when used from the time of viral infection. One hour pre-treatment of the cell monolayer with LF and subsequent addition of Rbv revealed a synergistic anti-hantaviral effect against SR-11, 〈20 FFU/ml as compared to 105 foci/ml in the control. One hour treatment of SR-11 with LF prior to cell inoculation gave an ED50 of 312.5 μg/ml. Whereas, washing the LF-pretreated cell monolayer with PBS demonstrated minimal focus reduction, suggesting LF lightly adheres to cells. These results indicate that LF has anti-hantaviral activity in vitro and inhibition of virus adsorption to cells which play an important role in revealing the anti-hantaviral activity of LF. This paper reports for the first time the anti-hantaviral effect of LF.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007050070077
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    Paper (German National Licenses)
    Fulltext
  • 13
    Electronic Resource
    Electronic Resource
    Protective immunity of Hantaan virus nucleocapsid and envelope protein studied using baculovirus-expressed proteins (1993)
    Springer
    Archives of virology 130 (1993), S. 365-376 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Recombinant Hantaan virus nucleocapsid protein (rNP) and recombinant envelope (rEnv) proteins were prepared using a baculovirus expression system to examine the role of Hantaan virus structural proteins in protective immunity. Passive transfer of spleen cells from mice immunized with rNP conferred partial protection or prolongation of time to death from fatal Hantaan virus infection in suckling mice which were challenged with Hantaan virus at 40 LD50 (survival rate: 43%) or 4 LD50 (survival rate: 43%). The T cell-enriched fraction protected one mouse from lethal infection but the B cell-enriched fraction had no such effect on fatal HTN infection. The protective effects of the antibody against HTN challenge were examined by passive immunization. The monoclonal antibody ECO 2 directed to NP also conferred partial survival and significant difference in time to death. Although rEnv antigen failed to induce neutralizing antibody, both immune spleen cells and immune serum to rEnv conferred partial protection upon suckling mice. These results indicate that both nucleocapsid and envelope proteins of Hantaan virus were responsible for induction of cell mediated protective immunity. Vero E 6 cells infected with Hantaan virus expressed envelope protein on the surface, as determined by flow cytometry. However, there was only negligible expression of nucleocapsid protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01309667
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    Paper (German National Licenses)
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  • 14
    Electronic Resource
    Electronic Resource
    Antigenic and genetic characterization of H1 influenza viruses isolated from feral ducks and swine in Japan (1983)
    Springer
    Archives of virology 78 (1983), S. 19-27 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The strains of H1N4 influenza A virus isolated from feral ducks in Japan in 1977–78 were compared to swine-origin H1N1 viruses antigenically and genetically. Homologous characteristics were found among the H1N4 isolates from feral ducks in hemagglutination-inhibition (HI) tests, viral RNA patterns on polyacrylamide gel electrophoresis and oligonucleotide mapping. Although the hemagglutinins of duck-origin viruses employed in this study were identified as H1, the viruses were distinguishable from A/New Jersey/8/76 (H1N1), A/duck/Alberta/35/76 (H1N1) and the virus isolated from swine in Japan in the cross HI test. Also, the viral RNA patterns of the duck- and swine-origin H1 viruses were found to be quite different, indicating that genetic reassortment of HA genes between them is unlikely. After H1N4 virus of duck-origin was intranasally inoculated into pigs, a brief period of virus recovery with no serological response was observed; whereas swine-origin H1N1 virus produced seroconversion in the pigs inoculated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01310855
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    Paper (German National Licenses)
    Fulltext
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