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  • 11
    Electronic Resource
    Electronic Resource
    Purification and characterization of 4-hydroxybenzoate 3-hydroxylase from a Klebsiella pneumoniae mutant strain (1995)
    Springer
    Archives of microbiology 164 (1995), S. 70-77 
    Details
    ISSN: 1432-072X
    Keywords: Key words 4-Hydroxybenzoate ; Monooxygenase ; Hydroxylase ; Klebsiella pneumoniae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Unlike the parent wild-type strain, the Klebsiella pneumoniae mutant strain MAO4 has a 4-HBA+ phenotype. The capacity of this mutant to take up and metabolize 4-hydroxybenzoate (4-HBA) relies on the expression of a permease and an NADPH-linked monooxygenase (4-HBA-3-hydroxylase). Both enzymes are normally expressed at basal levels, and only the presence of 4-HBA in the media enhances their activities. Strikingly, when the Acinetobacter calcoaceticus pobA gene encoding 4-hydroxybenzoate-3-hydroxylase was expressed in hydroxybenzoate K. pneumoniae wild-type, the bacteria were unable to grow on 4-HBA, suggesting that the main difference between the wild-type and the mutant strain is the capability of the latter to take up 4-HBA. 4-HBA-3-hydroxylase was purified to homogeneity by affinity, gel-filtration, and anion-exchange chromatography. The native enzyme, which appeared to be a dimer of identical subunits, had an apparent molecular mass of 80 kDa and a pI of 4.6. Steady-state kinetics were analyzed; the initial velocity patterns were consistent with a concerted substitution mechanism. The purified enzyme had 362 amino acid residues, and a tyrosine seemed to be involved in substrate activation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050237
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  • 12
    Electronic Resource
    Electronic Resource
    An open comparision of the diabetogenic effect of deflazacort and prednisone at a dosage ratio of 1.5 mg:1 mg (1999)
    Springer
    European journal of clinical pharmacology 55 (1999), S. 105-109 
    Details
    ISSN: 1432-1041
    Keywords: Key words Deflazacort ; Prednisone ; Glucose tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Abstract Objective: To compare the diabetogenic effects of deflazacort (D) versus prednisone (PN) using a dosage ratio of 1.5 mg deflazacort:1 mg prednisone. Methods: Thirty-three patients suffering from various active connective tissue or chronic inflammatory diseases were randomized to be treated with D or PN, assuming a therapeutic equipotency ratio of 1.5 mg D:1 mg PN. Neither dosage nor glucocorticoid employed were modified during the study. Patients had not received steroid treatment during the month prior to their inclusion date. Fasting glucose, glycosilated haemoglobin and fructosamine were determined before and after 1 month of treatment. Non-diabetic patients were also submitted to an oral glucose tolerance test (OGTT) at entry and after 1 month. Results were compared by univariate, and multivariate tests to correct the effects of age, body mass index and diagnosis. Results: After 1 month of treatment there were no differences between D and PN in fasting glucose, glycosilated haemoglobin, or fructosamine. OGTT performed after treatment showed similar glucose values for both treatment groups. Patients treated with D had insulin levels at min 60 of the post-treatment OGTT which were higher than those treated with PN [114.1 (62.8) mcUI · ml−1 versus 73.5 (32.7) mcUI · ml−1, P = 0.049], but the difference lost its statistical significance in the multivariate analysis. Conclusion: D and PN have similar effects on glucose tolerance when an equipotency ratio of 1.5 mg D:1 mg PN is employed. Previous studies employing a ratio of 1.2:1 mg may have understimated the adverse effects of D on glucose metabolism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002280050603
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  • 13
    Electronic Resource
    Electronic Resource
    Evidence for the role of the diabatic heating in synoptic scale processes: a case study example (1997)
    Springer
    Annales geophysicae 15 (1997), S. 487-493 
    Details
    ISSN: 0992-7689
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract The quasigeostrophic theory is used to address the role of diabatic forcing in synoptic scale processes over Iberia. A parametrization of diabatic heating is obtained in terms of a thermodynamic variable called the ice-liquid water potential temperature which is conservative under all phase changes of water. A case study objectively selected by means of a rotated principal component analysis over the diabatic field is analyzed to test the proposed parametrization. This study highlights the fact that the magnitudes of diabatic forcing and dynamic forcing are very nearly the same throughout the troposphere. The results also show that the composite diabatic heating is a better representation for both cloudiness and precipitation fields than the dynamic forcing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s00585-997-0487-2
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  • 14
    Electronic Resource
    Electronic Resource
    An overview of a heavy rain event in southeastern Iberia: the role of large-scale meteorological conditions (1997)
    Springer
    Annales geophysicae 15 (1997), S. 494-502 
    Details
    ISSN: 0992-7689
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract The heavy rain event of 30 January to 4 February 1993 in the Segura Basin in SE Iberia is investigated. The study emphasizes the atmospheric processes that led to thunderstorm development. The meteorological scenario has been separated into preconvective and convective periods and into large-scale and subsynoptic settings. The warm, moist air now from the Mediterranean Sea, channelled by a high-over-low blocking pattern with a cold front over southeastern Iberia appears to be the main reason that helped trigger a severe weather occurrence. The study of the mesoscale environment parameters showed an enhanced conditional unstability through a deep troposphere layer with a moderate to strong vertical wind shear that promoted conditions to allow organization of long-lived convective structures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s00585-997-0494-3
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  • 15
    Electronic Resource
    Electronic Resource
    Scleromyxoedema and thrombotic thrombocytopaenic purpura: two rare conditions both responding to plasma exchange (1998)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of the European Academy of Dermatology and Venereology 11 (1998), S. 0 
    Details
    ISSN: 1468-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: We report the case of a 66-year-old female who over an 18-month period developed severe, disabling scleromyxoedema with pulmonary fibrosis. Treatment with oral prednisolone and melphalan had failed to prevent disease progression. Treatment with a 5-day course of plasma exchange, intravenous cyclophosphamide (500 mg) and methyl-prednisolone (1 g on 3 consecutive days) was unfortunately followed by the development of thrombotic thrombocytopaenic purpura (TTP). After 17 extra plasma exchanges, she recovered and there has been a dramatic improvement in her skin signs. We postulate that the extra plasma exchanges which she received as a consequence of developing TTP have contributed to this result. To our knowledge, TTP has never been associated with scleromyxoedema, but it is likely to be a coincidence in this case.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1468-3083.1998.tb00956.x
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  • 16
    Electronic Resource
    Electronic Resource
    COMMUNITY RESPONSE TO STORMWATER POLLUTION IN AN URBANIZED WATERSHED (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of the American Water Resources Association 31 (1995), S. 0 
    Details
    ISSN: 1752-1688
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Architecture, Civil Engineering, Surveying , Geography
    Notes: : A model is developed and tested to identify the factors influencing variation in community response to stormwater pollution. Basic constructs of the model use environmental hazard theory, integrated with analysis of community demographic and political forces affecting local government decision processes. Community stormwater response is measured by an index comprised of 12 response indicators. Selection criteria for the response indicators include their effectiveness, variability, and general representation of the spectrum of stormwater response activity. Observable activities are scored progressively based upon their relative ecosystem impacts. Weighted and unweighted indicator scores are computed, with a survey of stormwater experts providing the basis for the weights. Results indicate a wide variation in response levels among the selected communities. Regression analysis identifies four significant predictors of response at the watershed scale: the mean age of householder over 25, the total tax rate, community type (city or township), and the amount of green staff (presence of recycling coordinators, foresters, or environmental planners). This mix of predictors demonstrates the applicability of hazard theory to stormwater response, as community stormwater response is influenced by the capacity and economic means to effect change - two central constructs of hazard theory.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1752-1688.1995.tb04036.x
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  • 17
    Electronic Resource
    Electronic Resource
    QUALITY STUDY OF GRAYWATER TREATMENT SYSTEMS (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of the American Water Resources Association 31 (1995), S. 0 
    Details
    ISSN: 1752-1688
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Architecture, Civil Engineering, Surveying , Geography
    Notes: : A residential single family dwelling was retrofitted to recycle graywater for landscape irrigation and toilet flushing. The objective of this study was to determine improvements in graywater quality by evaluating five simple graywater treatment systems that were easily adapted to the household plumbing. The treatment systems consisted of (1) water hyacinths and sand filtration, (2) water hyacinths, copper ion disinfection, and sand filtration, (3) copper ion disinfection and sand filtration, (4) copper/silver ion disinfection and sand filtration, and (5) 20–μm cartridge filtration. Water quality parameters measured were fecal and total coliform indicator bacteria, nitrates, suspended solids, and turbidity. Reductions in bacterial concentration, suspended solids and turbidity were achieved by all systems tested. Treatment reduced nitrate concentrations to an average of 2.6 mg/liter. Reductions in suspended solids, and turbidity were influenced more by the quality of the graywater entering the treatment system than the efficiency of the systems themselves. The water hyacinths and sand filtration system provided the best graywater quality in terms of the concentrations of fecal indicator bacteria. The system providing the best water quality in regard to average suspended solids after treatment was the water hyacinths, copper ion, and sand filtration system, and the best average turbidity was achieved by the copper/silver ion generating unit with sand filtration. All systems were capable of significant reductions in fecal indicator bacteria, suspended solids, and turbidity; however, additional treatment or disinfection would be necessary to further reduce the level of coliform and fecal coliform bacteria to achieve regulatory standards in the State of Arizona.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1752-1688.1995.tb03368.x
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  • 18
    Electronic Resource
    Electronic Resource
    Aggressive polyfibromatosis: A 10 year follow-up (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Australasian journal of dermatology 37 (1996), S. 0 
    Details
    ISSN: 1440-0960
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Polyfibromatosis syndrome is a condition characterized by the occurrence of several cutaneous fibrotic conditions including Dupuytren's contracture and keloid formation. A 10 year follow-up of a patient with an aggressive type of polyfibromatosis associated with erosive arthropathy is presented. The underlying pathogenesis and management of this uncommon condition is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1440-0960.1996.tb01056.x
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  • 19
    Electronic Resource
    Electronic Resource
    Analysis of current fluctuations in silicon pn+ and p+n homojunctions (1996)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 79 (1996), S. 6975-6981 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: A detailed study of bipolar transport under forward-bias conditions is evaluated by Ensemble Monte Carlo simulation in two structures (p+n and pn+ junctions). The static characteristics of both structures are presented. In particular, this study focuses on a microscopic analysis of current fluctuations. A decomposition of the autocorrelation function of the total current fluctuations in electron, hole and crossed contributions is performed. In this way, the importance of each type of carrier in the spectral density of current fluctuations in both structures for a wide frequency range is determined. In the low frequency range, the presence of shot, thermal and excess noise was found. The Ensemble Monte Carlo method also permits ready evaluation of the noise equivalent temperature in both structures. © 1996 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.361462
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  • 20
    Electronic Resource
    Electronic Resource
    Purification and Characterization of Guanine Nucleotide-Exchange Factor, eIF-2B, and p37 Calmodulin-Binding Protein from Calf Brain (1995)
    Oxford, UK : Blackwell Science Ltd
    Journal of neurochemistry 65 (1995), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Eukaryotic initiation factor 2B, or guanine nucleotide-exchange factor, has been purified for the first time from the brain by a novel procedure that allows the purification of initiation factor 2 as well and uses a salt wash postmicrosomal supernatant as starting material. The procedure includes a three-part chromatographic step in heparin-Sepharose and in SP-5PW and diethylaminoethyl-5PW ion-exchange high-performance chromatographies. The purification of the factors was followed by measuring activity in the guanine nucleotide-exchange assay and the capacity of initiation factor 2 to form a ternary complex with the initiation form of methionyl-tRNA and GTP. The method yields guanine nucleotide-exchange factor (75%) and highly purified initiation factor 2 (〉95%), which are separated in the last step. The exchange factor from the brain is a multimeric protein with five subunits of molecular masses of 82, 65, 52, 42, and 30 kDa; it stimulates ternary complex formation in the presence of GDP, and this activity is inhibited by N-ethylmaleimide. A 37-kDa protein that copurifies with initiation factors is characterized in this study as a new calmodulin-binding protein (p37); it is highly phosphorylated by casein kinase activities and can comigrate with the α subunit of initiation factor 2 under standard sodium dodecyl sulfate electrophoresis conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1471-4159.1995.65020754.x
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