Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 11
    Electronic Resource
    Electronic Resource
    Combined protein and DNA measurements by the ninhydrin-Schiff and Feulgen techniques (1979)
    Springer
    Histochemistry and cell biology 63 (1979), S. 35-45 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Feulgen nuclear staining with pararosanilin-SO2 was combined with the ninhydrin-Schiff technique. The aldehyde groups converted from primary amino groups are stained with an acriflavine-Schiff reaction. This results in a red nuclear fluorescence and a bright yellow cytoplasmic and nuclear fluorescence. The combined fluorescence staining facilitates cytofluorometric determination of total protein and DNA in the same cell. The ninhydrin-Schiff reaction is affected by the fixation procedure and the duration of the ninhydrin reaction. Investigations with a model system showed that proportionality beween the fluorescence intensity of acriflavine and the amount of protein stained by the procedure was obtained after fixation with a fixation mixture suggested by Böhm et al. (1968) and a reaction with ninhydrin at 37° C for 10 h. The ninhydrin-Schiff reaction has no effect on the fluorescence intensity of cells previously treated with pararosanilin-Feulgen staining and it is not affected itself by this previous procedure. Testing this double fluorescence staining on cytology specimens taken from patients with gastric carcinoma and uterine cervial carcinoma, cancer cells were shown to have markedly increased protein and DNA contents compared with those of normal cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00508010
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 12
    Electronic Resource
    Electronic Resource
    Altersabhängigkeit des Toluidinblau-Dichroismus und der Anisotropie der Fasern in der Wachstumszone menschlicher Rippen (1978)
    Springer
    Histochemistry and cell biology 59 (1978), S. 45-53 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die parallel zu den Säulenknorpelzellen in der Knorpelmatrix der Wachstumszone menschlicher Rippen verlaufenden Faserbündel untersuchten wir nach standardisierter Toluidinblau-Färbung absorptionsoptisch mit dem Leitz-Mikrospektrographen und polarisationsoptisch mit einem Berek- und einem Brace-Köhler-Kompensator. Bei 35 Autopsiefällen zwischen 0 und 22 Jahren wurde der Gangunterschied der Doppelbrechung an ungefärbten und an gefärbten Schnitten und der Dichroismus der Faserbündel an gefärbten Präparaten bestimmt. Der metachromatische Index wurde berechnet. Für alle Parameter ergab sich eine charakteristische altersabhängige Verteilung der Meßwerte, die darauf hinweist, daß die höchste molekulare und strukturelle Ordnung der Faserbündel bereits im 8. Lebensjahr erreicht wird. Der mit dem Lebensalter kintinuierlich zunehmende Gangunterschied kann durch den progredienten Wasserverlust der Fasern erklärt werden.
    Notes: Summary The chondral fibers running parallel to the chondrocyte columns within die costo-chondral junction of human ribs were investigated quantitatively following standardized Toluidine blue staining by using the Leitz-Mikrospektrograph for observing the absorption spectrum, and by employing both a Berek- and a Brace-Köhler compensator for polarization measurements. Autopsy material was obtained from the ribs of 35 children and adults at the age of 0 to 22 years. Refractivity was measured in stained and unstained paraffin sections, whereas dichroism and metachromatic index (MI) of the fibers were determined in stained specimens. All three parameters showed characteristic, quantitative age-dependent variations indicating that the highest degree of molecular and structural adjustment and alignment of the fibers is already being reached at the age of 8 years. The continuously higher refractivity of the fibers with increasing age and bone maturity may be explained by a progressive loss of water by the fibers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00506476
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 13
    Electronic Resource
    Electronic Resource
    Proportionalitätsfehler bei der Feulgen-Hydrolyse (1968)
    Springer
    Histochemistry and cell biology 15 (1968), S. 194-203 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Beim Vergleich des Feulgen-Farbgehaltes verschiedener Zellkerne (Leberzellen, Lymphozyten, Granulozyten und Spermien) traten nach Alkoholfixierung Abweichungen der gemessenen Feulgen-Werte von den nach dem Gesetz von der DNS- Konstanz zu erwartenden DNS-Gehalt dieser Kerne auf. Verglichen mit den Feulgen-Werten der diploiden Leberzellkerne ergaben Lympho- und Granulozyten bei allen Hydrolysezeiten zu niedrige (bis zu minus 20%), haploide Spermien im postmaximalen Hydrolysebereich zu hohe Feulgen-Werte (z. T. sogar höhere Werte als die diploiden Zellkerne). Innerhalb desselben Zelltypes wurden dagegen, auch beim Vergleich der verschiedenen Ploidiestufen der Leberkerne, keine Differenzen festgestellt. Da die an Leukozyten und Spermien beobachteten Abweichungen nach Methanol-Formalin-Eisessig-Fixierung nicht mehr auftraten und auch durch UV-absorptionscytophotometrische Messungen nicht bestätigt werden konnten, muß man annehmen, daß es sich um Proportionalitätsfehler handelt, die auf Hydrolyseunterschieden beruhen. Für die quantitative Feulgen-Cytophotometrie scheint daher die Methanol-Formalin-Eisessig-Fixierung besser geeignet zu sein als die Alkoholfixierung, bei deren Verwendung es leicht zu Proportionalitätsfehlern während der Feulgen-Hydrolyse kommen kann.
    Notes: Summary Comparing the Feulgen dye-content of different nuclei (liver cells, lymphocytes, granulocytes and sperms) after alcohol-fixation deviations were found between the measured Feulgen values and the DNA-content to be expected from the DNA-constancy law. The Feulgen values of lymphocytes and granulocytes proved to be lower (up to minus 20%) than those of diploid liver nuclei at all hydrolysis times, while in the postmaximal range of hydrolysis the values of the haploid sperms were too high (even higher than those of the diploid nuclei). Such differences did not appear when nuclei of the same cell type in different DNA- ploidy classes (liver nuclei) were compared. Those deviations of leucocytes and sperms were no longer found after fixation in methanol-formalin-glacial acetic acid and, in addition, could not be confirmed by UV-absorption measurements. For that reason we suppose them to be due to proportionality errors caused by variations in the hydrolytic behaviour of the different nucleoproteins. Thus fixation in methanol-formalin-glacial acetic acid seems to be more suitable for quantitative Feulgen measurements than alcohol-fixation, which may easily give rise to proportionality errors during Feulgen hydrolysis. Moreover, to avoid any false interpretation of Feulgen data we should suggest controll measurements using another independent method (f. e. UV-absorption), or — if this is impossible — to check the Feulgen values after different fixations and variant hydrolysis times (premaximal, maximal, postmaximal).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00305883
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 14
    Electronic Resource
    Electronic Resource
    Einfluß der Fixierung und der Säurekonzentration auf die Feulgen-Hydrolyse bei 28° C (1968)
    Springer
    Histochemistry and cell biology 14 (1968), S. 201-211 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Es wurde eine für Routinezwecke geeignete Feulgenreaktion für die cytophotometrische DNS-Bestimmung ausgearbeitet, die gegen geringe Schwankungen der Temperatur, der Säurekonzentration und der Zeitdauer der HCl-Hydrolyse weniger empfindlich ist als die 60°-Standardhydrolyse. Dazu wurden die Feulgen-Hydrolysekurven von alkohol-, formalin- und methanol-formalin-eisessigfixierten Hühnererythrocyten nach Behandlung mit 5 N, 4 N und 2 N HCl bei 28° C, bzw. mit 1 N HCl bei 60° C geprüft und miteinander verglichen. Als besonders brauchbar erwies sich die Fixierung mit 70%igem Isopropylalkohol (20 min Hydrolyse in 5 N HCl oder 45 min in 4 N HCl) und die MethanolFormalin-Eisessig-Fixierung (105 min Hydrolyse in 4 N HCl). Reine Formalinfixierung erwies sich als ungeeignet, da eine starke Kernschrumpfung mit Extinktionen größer als 0,75 beobachtet wurden. An alkoholfixierten Leberzellausstrichen wurde ein gleichartiger Verlauf der Hydrolysekurven von di-, tetra- und oktoploiden Leberzellkernen festgestellt. Die relativen Farbstoffmengen (AE-Werte) dieser Kerne verhielten sich bei allen geprüften Hydrolysezeiten wie 1∶2∶4.
    Notes: Summary A routine Feulgen procedure for quantitative cytophotometric absorption measurements of DNA at the integrating microdensitometer should be established, which is less alterable by minor deviations in temperature, acid concentration and in duration of hydrolysis than is the 60° C standard treatment. Feulgen hydrolysis curves of alcohol-, formalin- and methanol-formalin-glacialacidicacid-fixed fowl erythrocytes have been examined after hydrolysis in 5 N, 4 N and 2 N HCl at 28° C, as well as in 1 N HCl at 60° C. Fixation in 70% isopropylalcohol and hydrolysis in 5 N HCl for 20 minutes or in 4 N HCl for 45 minutes proved to be particularly useful. Fixation in a mixture of 85% methanol, 10% formalin and 5% glacialacidicacid gave good results, too, but hydrolysis time had to be chosen considerably longer for maximum staining (105 minutes in 4 N HCl). Formalin fixation proved not to be suitable because of a considerable shrinkage of the nuclei resulting in extinctions well above 0.75. Identical hydrolysis curves have been obtained for di-, tetra- and octoploid liver cell nuclei from alcohol-fixed liver smears. The relative dye contents (AE-values) of these nuclei were in the ratio 1∶2∶4 at all hydrolysis times examined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306341
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 15
    Electronic Resource
    Electronic Resource
    Fluoreszenzzytophotometrische Bestimmung der Zellkern-DNS (1972)
    Springer
    Histochemistry and cell biology 30 (1972), S. 255-267 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Akriflavin-Feulgen-Färbung (Böhm und Sprenger, 1968) und die Ethidiumbromid-Fluorochromierung nach vorausgehender Pepsinbehandlung (Göhde et al., 1971) ergeben an Leberzellausstrich-Präparaten weitgehend gleichartige DNS-Häufigkeitsverteilungen. Bei der zeitlich aufwendigen (220 min) Akriflavin-Färbung ist eine optimale Darstellung der Zellmorphologie und eine jahrelange Archivierung der Ausstrichpräparate möglich. Die rasch durchführbare Ethidiumbromid-Färbung (70 min) bietet nur nach schwerer Alteration der Zellmorphologie durch die vorausgehende Pepsinbehandlung eine selektive Darstellung der DNS. Eine Archivierung von Ausstrichpräparaten ist nur für 2 Tage möglich. Die schwere Alteration der Zellmorphologie ist besonders bei durchflußphotometrischen Prescreeninguntersuchungen in der gynäkologischen Krebsvorsorge nachteilig. Zellpopulationen, die zu einer zytophotometrischen Verdachtsdiagnose geführt haben, sind durch Verlust des Zytoplasmas und der Kernstruktur einer konventionellen morphologischen Diagnostik nicht mehr zugängig.
    Notes: Summary Acriflavine-Feulgen (Böhm und Sprenger, 1968) and ethidiumbromide fluorescence after previous pepsin digestion (Göhde et al., 1971) yield corresponding DNA distribution patterns when applied to liver smears. With the time-consuming acriflavine staining (220 min) the cellular morphology is best preserved and the stained specimen may be stored for long periods. The rapidly obtainable ethidiumbromide staining (70 min) results in a selective fluorescence of DNA only after heavy alteration of the cellular morphology by pepsin digestion. Storage of the material is only possible for two days. The heavily altered cellular morphology, however, is rather unfavorable for prescreening in automated cytology. Specimens that were found to be suspicious are no longer suitable for conventional cytological diagnosis, because they have lost their cytoplasm and nuclear chromatin structure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00277596
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 16
    Electronic Resource
    Electronic Resource
    Zur Bestimmung der Parameter der Bateman-Funktion bei der Auswertung von Feulgen-Hydrolysekurven (1966)
    Springer
    Histochemistry and cell biology 6 (1966), S. 260-266 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Im Zusammenhang mit der Auswertung von Feulgen-Hydrolysekurven wird ein Regressionsverfahren dargestellt, bei dem die Bateman-Funktion $$y = \frac{{y_0 \cdot k_1 }}{{k_2 - k_1 }}[\exp ( - k_1 t)] - \exp ( - k_2 t)]$$ zugrunde gelegt wird. Das Schätzen der Parameter erfolgt durch Lösen eines nichtlinearen Gleichungssystems. Diese geschieht durch näherungsweise Bestimmung der Nullstellen einer Funktion mittels eines modifizierten Newton-Raphson-Verfahrens. Das Regressionsverfahren wurde programmiert im Freiburger Code für die elektronische Rechenanlage Zuse Z 23 und im Fortran II für die Anlage IBM 7090. Für die praktische Auswertung mit Hilfe dieser Programme werden Beispiele angegeben.
    Notes: Summary In connection with the evaluation of Feulgen hydrolysis curves a regression procedure is described which is based on the Bateman function $$y = \frac{{y_0 \cdot k_1 }}{{k_2 - k_1 }}[\exp ( - k_1 t)] - \exp ( - k_2 t)]$$ . The parameters are estimated by solving an equation which is not linear. This is done by approximately determining the zero position of a function by means of a modified Newton-Raphson-Procedure. The regression procedure programme was set up in the Freiburg code for the computer Zuse Z 23 and in the Fortran II for the IBM 7090 computer. Examples are given for the practical evaluation with these programs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01257323
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 17
    Electronic Resource
    Electronic Resource
    Excessive reversible phenobarbital induced nuclear DNA-polyploidization in the growing mouse liver (1981)
    Springer
    Histochemistry and cell biology 72 (1981), S. 63-74 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Phenobarbital was injected intraperitoneally into male white NMRI mice aged 0.5, 1, 1.5, 3, 6 and 12 months at a dose of 120 mg/kg body weight for 10 consecutive days. The 0.5 month-old mice did not tolerate the phenobarbital dose and died. The experimental animals and one of the controls were sacrificed 1, 3, 5, 10, 15 and 20 days after phenobarbital administration was started. Liver weights were recorded and liver cells were isolated. The number of nuclei per cell was determined and the DNA-content of each single nucleus was measured by Feulgen fluorescence cytophotometry. Liver weights showed an increase of 25–30% during phenobarbital treatment and returned slowly to lower values after cessation of drug application. The hepatic enlargement was accompanied by an excessive and likewise reversible nuclear and whole cell DNA-polyploidization, i.e. polyploidization beyond the physiological age-dependent ploidy level; for example, mean values of 7.7 c per nucleus (versus 4.2 c in the controls) and 14.3 c for whole liver cells (versus 7.5 c in the controls) were found in 3 months-old animals after 5 days of treatment. As with the induction of microsomal enzymes, the augmentation of smooth endoplasmic reticulum, and the increase of RNA- and protein-synthesis, excessive DNA-polyploidization of liver cell nuclei appears to be an expression of hepatocellular hypertrophy due to the functional or metabolic stress imposed upon the liver by large quantities of phenobarbital. After cessation of drug administration the abnormally high ploidy cells are eliminated-probably by necrobiosis — and the liver cells return to their normal age-dependent DNA-ploidy level. The liver cells of the one-month-old animals revealed the physiological polyploidization to be slightly inhibited. This is probably due to some toxic effect of phenobarbital. Phenobarbital did not alter the number of nuclei per liver cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00496780
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 18
    Electronic Resource
    Electronic Resource
    Development of binuclearity and DNA-polyploidization in the growing mouse liver (1981)
    Springer
    Histochemistry and cell biology 72 (1981), S. 55-61 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Suspensions of intact liver cells were prepared from 36 male NMRI mice of different age after perfusion of the liver with ice-cold calcium- and magnesium-free phosphate buffer (CMF). The suspensions of the isolated hepatocytes were smeared on slides, fixed, hydrolized and stained by fluorescent acriflavine-Schiff-Feulgen reaction. The number of nuclei per cell was determined in a phase-contrast microscope. Quantitative fluorescent cytophotometric measurements of nuclear Feulgen-DNA were performed on individual nuclei. At the age of 0.5 month, 55% of the hepatocytes were found to be mononuclear, 45% binuclear. In the animal groups aged 1 month, 1.5 months, 3 months, 6 months and 12 months, the percentage of binuclear hepatocytes remained constant at about 80%. Very few liver cells with 3 or 4 nuclei were detected. Feulgen-DNA-measurements revealed a predominance of 2c and 4c nuclei at ages 1 month and 1.5 months with logarithmic increase of 8c nuclei and a decrease of the 2c nuclei. From 1.5 months on 16c nuclei were found, which never exceeded 8%. When total DNA-ploidy of the hepatocytes was calculated similar kinetics at a higher ploidy level were observed. 2c hepatocytes existed in small percentages at very young ages up to 1.5 months, but were also occasionally found in older animals. With increasing age the number of 16c hepatocytes increased logarithmically with a concomitant decrease of the 4c hepatocytes. The percentage of 8c liver cells remained more or less constant. Few hepatocytes with a 32c total DNA content were found in mice aged 3 months and older. In one-year-old mice the mean DNA-ploidy was calculated to be 5.8c per liver nucleus and 10.0c per whole hepatocyte.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00496779
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 19
    Electronic Resource
    Electronic Resource
    Alveoläres Adenom der Lunge Immunhistochemische Charakterisierung von Pneumozyten Typ II (1996)
    Springer
    Der Pathologe 17 (1996), S. 150-153 
    Details
    ISSN: 1432-1963
    Keywords: Schlüsselwörter Alveoläres Adenom ; Immunhistochemie ; Surfactantapoproteine B und C ; Alveozyten Typ II ; Key words Alveolar adenoma ; Immunohistochemistry ; Surfactant apoproteins B and C ; Alveocytes type II
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The alveolar adenoma of the lung is a rare benign tumor in which the normal parenchymal architectur is imitated by a proliferation of both the alveolar epithelial cells and the mesenchymal septal cells. The first description, based on six cases, was published in 1986 by Yousem and Hochholzer. From their ultrastructural findings they presumed a type II pneumocytes differentiation of the epithelial cells. We investigated an alveolar adenoma of the lung immunhistochemical by means of antibodies against apoprotein B and C of human surfactant. Both the lining cells and the macrophages in the alveolar-like spaces were stained. The septal connection tissue cells did not react. These findings confirm the expression of surfactant constituants and, hence, the differentiation into type II pneumocytes of the epithelial cells of the alveolar adenoma.
    Notes: Zusammenfassung Das alveoläre Adenom der Lunge ist ein seltener gutartiger Tumor, der als kombinierte Proliferation alveolärer epihelialer Zellen und septalen Mesenchyms angesehen wird und normale Lungenparenchymarchitektur nachahmt. Es wurde 1986 zum ersten Mal anhand von 6 Fällen von Yousem u. Hochholzer [11] beschrieben. Die von den Autoren an 2 Fällen durchgeführten ultrastrukturellen Untersuchungen wiesen auf eine Differenzierung der epithelialen Komponente in Richtung Alveozyten Typ II hin. Wir konnten an einem Fall eines alveolären Adenoms immunhistochemische Untersuchungen mit einem Antikörper gegen die Apoproteine B und C des humanen Surfactant durchführen (zur Verfügung gestellt von der Firma Thomae). Dabei zeigten die Epithelzellen, die alveolenähnliche Hohlräume auskleiden sowie in diesen Hohlräumen liegende Makrophagen eine positive Reaktion, wohingegen die Zellen der Bindegewebssepten negativ reagierten. Der immunhistochemische Nachweis der Surfactantapoproteine B und C im Zytoplasma der epithelialen Tumorzellen bestätigen deren Differenzierung in Richtung Alveozyten Typ II.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002920050149
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 20
    Electronic Resource
    Electronic Resource
    Focal necroses, fatty degeneration and subendocardial nuclear polyploidization of the myocardium in newborns after β-sympathicomimetic suppression of premature labor (1981)
    Springer
    European journal of pediatrics 136 (1981), S. 149-157 
    Details
    ISSN: 1432-1076
    Keywords: β-Sympathicomimetics ; Tocolysis ; Myocardial lesions ; Cytophotometry ; Nuclear DNA poly-ploidization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract It has been well documented in laboratory animals that β-sympathicomimetics, such as isoprenalin, can cause myocardial lesions. Other so called “β2-selective” symphaticomimetic drugs, which nevertheless induced β1-cardiostimulatory side effects, are now widely used for suppression of premature labor. We examined the hearts of 25 newborns whose mothers had been treated with β-sympathicomimetics for various lengths of time (24 h to 8 weeks). Three types of lesions were detected: (1) focal subendocardial necroses (3 cases), similar to isoprenalin-induced myocardial necroses in animal experiments, (2) diffuse fatty degeneration of myocardial cells (3 cases), and (3) nuclear polyploidization in the subendocardial layer of the right ventricular wall (14 cases). However, the immediate causes of death could not be directly related to the tocolytic treatment in any of the cases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00441917
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...