ISSN:
1440-1681
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Medizin
Notizen:
1. The purpose of the present study was to investigate the changes in superoxide dismutase (SOD) isoenzyme (Mn2+-SOD and Cu2+, Zn2+-SOD) activities, contents and mRNA expressions in rat skeletal muscle during endurance training and a single bout of exercise.2. Thirty-eight male Wistar rats were divided into untrained (U) and trained (T) groups. The T group rats were treadmill-trained for 9 weeks. The activity, content and mRNA expression of Mn2+-SOD and Cu2+, Zn2+-SOD were determined in the soleus muscle of each rat.3. Mn2+-SOD activity and content in the T group were significantly higher than in the U group, both at rest (22 and 21%, respectively) and after exercise (24 and 46%, respectively), while a single bout of exercise affected neither the activity nor content of Mn2+-SOD in either group.4. The content of Cu2+, Zn2+-SOD in both groups was not different at rest and after exercise, although its activity at rest was significantly higher in the T group than in the U group (by 29%).5. After exercise, the expression of Mn2+-SOD mRNA was markedly attenuated only in the U group (49%); the expression of Cu2+, Zn2+-SOD mRNA was not influenced by exercise.6. Our results suggest that adequate endurance training increases both the activity and content of Mn2+-SOD and that untrained rats are rather susceptible to oxidative stress during physical exercise. It thus appears that Mn2+-SOD provides a reliable index of physical training.7. The results obtained in the present study also suggest that muscle has the capacity of responding to training in such a manner as to reduce the potential harm arising from the accumulation of oxygen free radicals resulting from enhanced metabolic activity.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1111/j.1440-1681.1997.tb01196.x
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