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  • 1
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 120 (2001), S. 0 
    ISSN: 1439-0523
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Random amplified polymorphic DNA (RAPD) analysis was applied to eight commercial cultivars of pineapple, two intergroup hybrids and two wild species. Morphologically, pineapple is divided into the Cayenne, Queen, Spanish, Maipure and Abacaxi groups. Members of the first three groups have been analysed in this study. The cultivars ‘Tradsithong’, ‘Phuket’, ‘Sawee’ and ‘Tainan’, with spiny leaves, form the Queen group. In ‘Pattavia’, ‘Nanglae’ and ‘Petburi no. 2’ (Cayenne group), spines are confined to the leaf tips. ‘Intrachitdang’ is normally placed in the Spanish group, which is morphologically similar to the Queen group, but with inferior quality fruit. DNA amplification products were compared from 16 arbitrary 10-mer primers from which a dendrogram was constructed. The results confirmed morphological classifications for seven of the eight commercial cultivars, with the Queen and Cayenne groups as separate clusters. However, the cv. ‘Intrachitdang’ was more closely related to the Cayenne group. Two hybrids from reciprocal Cayenne × Queen group crosses, were more closely allied to the Queen group. The two wild species were outside the groups. RAPD analysis can be exploited to investigate relationships within pineapple germplasm.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    ISSN: 1432-203X
    Schlagwort(e): Pluronic F-68 ; Callus growth ; Protoplasts ; Solanum dulcamara
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The effects of the non-ionic surfactant, Pluronic F-68, on the growth of callus and protoplasts from Solanum dulcamara L. have been studied. Growth of callus was stimulated by addition of 0.1% (w/v) commercial grade Pluronic to culture medium, whereas lower concentrations (0.01% w/v) had no corresponding effect. In contrast, higher concentrations (1.0% w/v) of Pluronic inhibited callus growth. The mean plating efficiency of protoplasts grown at different densities (15 days after plating) was increased up to 26% following culture with 0.1% (w/v) Pluronic, while 0.01% (w/v) Pluronic was ineffective. Mean protoplast plating efficiency decreased by up to 32% following culture with 1.0% (w/v) Pluronic.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    ISSN: 1432-203X
    Schlagwort(e): Corchorus capsularis ; jute ; cotyledons ; surfactants ; Pluronic F-68 ; Triton X-100 ; Tween 20 ; shoot regeneration
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The effects have been studied of the non-ionic surfactants, Plutonic F-68, Tween 20 or Triton X-100, on shoot regeneration from cultured jute (Corchorus capsularis L.) cotyledons with attached petioles. This group of non-ionic surfactants was selected in order to determine a possible relationship between the physico-chemical properties of individual compounds and their observable effects on plant morphogenesis in cultured jute cotyledons. Supplementation of culture medium with 0.001–0.5% (w/v) Pluronic F-68 increased the mean percentage of cotyledons producing shoots and the mean number of shoots/cotyledon, with maximal responses at 0.5% (w/v). By contrast, Tween 20 produced maximal effects at 0.001% (v/v), with inhibition of shoot formation at 0.5% (v/v). In both cases, phenotypically normal plants were recovered which could be grown to maturity. Culture of cotyledons with 0.001% (v/v) Triton X-100 similarly increased both the percentage of cotyledons producing shoots and the number of shoots/cotyledon. However, these shoots did not develop into mature plants. Additionally, shoots did not regenerate from cotyledons cultured with Triton at 0.01–0.5% (v/v). These results demonstrate mat there is an apparent relationship between the hydrophilic-hydrophobic (HLB) balance of surfactants which determine their cell permeabilising properties and consequently, their effects on morphogenesis.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 4
    ISSN: 1432-203X
    Schlagwort(e): Petunia hybrida ; protoplasts ; oxygen delivery ; perfluorochemicals ; Pluronic F-68 ; surfactant ; cell division
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Cell suspension-derived protoplasts of albino Petunia hybrida were grown for 10 d at the interface between aqueous culture medium (KM8P) and an oxygenated (10 mbar for 15 min) perfluorocarbon liquid, perfluorodecalin. Protoplasts synthesised new cell walls and divided normally at the perfluorodecalin/culture medium interface, with a mean viability after 10 d of 〉 92.0%. The mean plating efficiency of protoplasts was elevated by 37% (P〈0.05) following culture at the perfluorodecalin/medium interface, but was unaltered by perfluorodecalin or oxygen separately. The mean plating efficiency of protoplasts cultured at the interface was further increased to a maximium of 52% above control, in the presence of oxygenated perfluorodecalin and KM8P medium supplemented with the non-ionic, co-polymer surfactant, Pluronic F-68 at 0.01% (w/v). These findings demonstrate the effectiveness of oxygenated perfluorodecalin for promoting protoplast growth, by facilitating oxygen delivery. The finding that Pluronic F-68 further increased the plating efficiency of protoplasts cultured at the perfluorocarbon/aqueous interface suggests that these agents improve growth through separate, but cumulative, mechanisms.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 5
    ISSN: 1432-203X
    Schlagwort(e): Key words Carbona dioxide ; Fluorocarbon ; Micropropagation ; Rosa chinesis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The inert perfluorochemical (PFC) liquid, perfluorodecalin (Flutec PP6), has been used to increase the CO2 supply to cultured shoots of Rosa chinensis Jacq. cv. Baby Love. Culture of shoots in semi-solid medium overlaying CO2-gassed PFC (2 mbar; 5 min repeated every 7 days) for up to 42 days, increased biomass as reflected by significant (P〈0.01) increases in shoot number, number of leaves per shoot and mean shoot fresh weight. Additionally, there were significant (P〈0.01) increases in the number of roots and their fresh and dry weights following a further 10 days of culture on rooting medium prior to transfer of plants to the glasshouse. Treatment of cultured rose shoots with CO2-gassed PFC also significantly reduced (P〈0.01) the accumulation of phenolic compounds in roots. The total chlorophyll of aerial parts was unaffected, although total protein in shoots and roots was significantly (P〈0.01) lower than in the control. The biotechnological implications of this novel cultural régime are discussed for the micropropagation of woody species.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 6
    ISSN: 1432-203X
    Schlagwort(e): Key wordsPetunia hybrida ; Oxygen delivery ; Erythrogen ; Haemoglobin ; Pluronic F-68
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Novel approaches, involving supplementation of aqueous culture medium with haemoglobin solution (Erythrogen), in the presence or absence of the copolymer surfactant, Pluronic F-68, have been evaluated to facilitate cellular oxygen availability to promote mitotic division. Cell-suspension-derived protoplasts of albino Petunia hybrida cv. Comanche were cultured for up to 45 days in KM8P medium containing 1:50–1:500 (vol:vol) Erythrogen. The mean initial protoplast plating efficiency after 9 days with 1:50 Erythrogen (18.5%) was significantly greater (P〈0.05) than in untreated controls (11.3%). Supplementation of culture medium with 1:50 Erythrogen, together with 0.01% (wt/vol) Pluronic F-68, increased the mean plating efficiency after 9 days (24.4%) by 92% (P〈0.05) over the control (12.7%). These treatments also produced increases in biomass of protoplast-derived cells up to 2.5-fold greater than control (P〈0.01) over 80 days. Gassing the medium, containing 1:50 Erythrogen, with carbon monoxide abolished the increase in plating efficiency. There was no additional benefit of gassing Erythrogen-supplemented medium with 100% oxygen. The synergistic, beneficial effect of Erythrogen and Pluronic F-68 on protoplast division has implications for plant biotechnology utilising protoplasts.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 7
    ISSN: 1432-203X
    Schlagwort(e): Key wordsAgrobacterium-mediated transformation ; Datura meteloides ; Preculture period ; Transgene expression
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A transformation system is described for Datura meteloides using the supervirulent Agrobacterium tumefaciens strain 1065, carrying both the β-glucuronidase (gusA) and neomycin phosphotransferase II (nptII) genes between the T-DNA border sequences of the binary vector. The importance of conditions such as the preculture period of the plant tissues, wounding, bacterial dilution and incubation time were evaluated in terms of transgenic plant production. A preculture period of 2–3 days, using a 1:20 or 1:10 (vol:vol) dilution of an overnight bacterial culture, resulted in optimum shoot regeneration, with 48% from a total of 576 explants regenerating transformed shoots. Expression of the gusA and nptII genes was confirmed by a GUS fluorometric assay and by NPTII ELISA. Southern analysis revealed the integration of both transgenes, which segregated as dominant Mendelian traits in seed progeny.
    Materialart: Digitale Medien
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  • 8
    Digitale Medien
    Digitale Medien
    Springer
    Journal of molecular medicine 63 (1985), S. 1028-1034 
    ISSN: 1432-1440
    Schlagwort(e): Perfluorocarbons ; Blood replacement ; Adverse reactions ; Leukocytes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The effects of near total blood replacement with the proprietary perfluorocarbon emulsion, Fluosol-DA, have been examined in conscious, chronically catheterized rats. Animals survived for up to 48 h with a haematocrit of 〈2%. No overt changes in cardiovascular or respiratory functions occurred during blood replacement but thereafter, animals showed a progressive deterioration of haemodynamic control. Changes in intravascular fluid composition during and following exchange-transfusion have been monitored and the extent to which these changes could have contributed to the eventual disruption of homeostasis is discussed.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 9
    Digitale Medien
    Digitale Medien
    Springer
    Cellular and molecular life sciences 40 (1984), S. 700-701 
    ISSN: 1420-9071
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The effects of exchange-transfusion with a proprietary gelatin-based plasma volume expander, Haemaccel, have been investigated in conscious, chronically-catheterized rats. No adverse changes in basic cardiovascular or respiratory functions occurred during the procedure although an increase in intravascular fluid sodium but not potassium concentration was observed.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 10
    Digitale Medien
    Digitale Medien
    Springer
    Cellular and molecular life sciences 42 (1986), S. 1228-1231 
    ISSN: 1420-9071
    Schlagwort(e): Blood replacement ; perfluorocarbon emulsion ; Fluosol-DA ; lactate dehydrogenase ; alkaline phosphatase ; rats
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The effects of near total blood replacement with the proprietary perfluorocarbon (PFC)-based emulsion, Fluosol-DA 20%, on plasma concentrations of 2 enzymes, lactate dehydrogeanse (LDH) and alkaline phosphatase (ALP), have been examined in conscious, chronically catheterized rats. A pronounced fall in both plasma LDH (p〈0.05) an ALP (p〈0.01) occurred in response to exchange-transfusion. However, at 6 h following blood replacement, plasma concentrations of both enzymes had risen to values significantly greater than those measured immediately before perfusion. The observed changes in plasma LDH and ALP after blood replacement with Fluosol-DA indicated alterations in normal functioning of tissues from which these enzymes originate.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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