ISSN:
1600-0714
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Medizin
Notizen:
An immunoperoxidase staining technique was used for detecting alpha one-antichymotrypsin (αl-ACT), alpha one-antitrypsin (αl-AT), lactoferrin and transferrin in routine histological paraffin sections of 30 adenolymphomas, as well as in normal salivary gland tissue. Microscopically, the epithelial, component of adenolymphomas consisted of tall columnar luminal cells and irregularly shaped basal cells. αl-ACT was detected in the luminal layer of epithelium in 27 (90%) of 30 adenolymphomas, while the basal layer was positive in 4 cases (13%). Lactoferrin could be observed in the columnar cells of 21 cases (70%) and was positive in the basal cells of 2 cases (7%). In normal salivary gland tissue, αl-ACT and lactoferrin were observed in the intercalated duct and serous acinar cells. The staining pattern of αl-AT in adenolymphoma was similar to those of αl-ACT and lactoferrin, however, the number of positive cases for αl-AT was fewer than in the αl-ACT and lactoferrin. αl-AT was not found in the normal salivary gland. On the contrary, the localization of transferrin in the epithelial component of adenolymphomas was exclusively different from those of αl-ACT, αl-AT and lactoferrin. Transferrin was found more often in the basal cells than in the tall columnar apical cells. The staining pattern of transferrin in the normal salivary gland was different from αl-ACT and lactoferrin, and transferrin was positive in the cytoplasm of intercalated ducts, serous acinar and myoepithelial cells. Therefore, concomitant use of these 4 immunohistochemical stains may offer a way to study the functional condition of two-layer epithelium of adenolymphoma. These results would indicate an intimate iinmunohistochemical expression between the normal intercalated duct and serous acinar cells and the epithelial component of adenolymphomas.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1111/j.1600-0714.1988.tb01538.x
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