Digitale Medien
Oxford, UK
:
Blackwell Publishing Ltd
Scandinavian journal of immunology
34 (1991), S. 0
ISSN:
1365-3083
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Medizin
Notizen:
IgG1 is cleaved in vitro by granulocyte elastase into Fc and Fab fragments. The elastase-specific Fe fragment has been previously detected in vivo. Biological activity of the fragments has been described in modulating neutrophil oxidative metabolism and enzyme release. To investigate further effects granulocyte chemotaxis(CT) was tested. The CT was assayed in Boyden chambers and the chemotactic index (CI) was calculated which represents the mean distance travelled by the activated cells.Stimulation of leucocyte CT by casein, activated scrum and FMLP gives maximal values of Δ CI = 46.7, 26.4 and 7.1 μm, respectively. Native IgG1 and the elastase-produced IgG fragments do not stimulate leucocyte CT.FMLP-stimulated CT is specifically inhibited by the elastase-produced Fc fragments. Addition of 7 nmol Fc to stimulus concentrations of 16 to 125 nM FMLP results in total inhibition of chemotaxis demonstrated by CI values which are lower than those for unstitmulated cells. The inhibition of CT is concentration dependent in the range of 2 to 7 nmol Fc/106PMN.Number and affinity of FMLP receptors are not incipience by Fc fragments, so Fc binds neither to FMLP nor the FMLP receptor.CT stimulated by casein shows a large portion of chemokinesis. Only at suboptimal casein concentrations do Fc and IgG have an Inhibitory effect on CT (0.63 mg casein /ml. 10 nmol peptide/106 PMN).C5a-stimulaicd CT is not influenced by IgG or IgG fragments which indicates that the samples are not cytotoxic. So the FMLP and casein-stimulated CT is specifically inhibited by the elastase-produced Fc fragments in a low concentration range.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1111/j.1365-3083.1991.tb01557.x
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