Digitale Medien
Oxford, U.K. and Cambridge, USA
:
Blackwell Science Ltd
Scandinavian journal of immunology
52 (2000), S. 0
ISSN:
1365-3083
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Medizin
Notizen:
The γ/δ T cells play important roles as the first line of defence in mucosal immunity. Vδ1 bearing T cells comprise the major population of γ/δ T cells in the human nasal mucosa. Recently, it was reported that certain receptors are predominant at given anatomical locations. The purpose of this study was to elucidate the γ/δ T-cell receptor repertoire in human nasal mucosa. Nasal epithelia were scraped and peripheral blood were obtained from three healthy volunteers. We performed reverse-transcription polymerase chain reaction (RT-PCR) analysis and characterized the junctional sequences of Vδ1 T-cell receptor (TCR) transcripts. Vγ and Vδ gene usage in intraepithelial lymphocytes (IELs) in right and left nasal mucosa were almost the same, but they were different from those in peripheral blood lymphocytes (PBLs). The VDJ sequence of nasal Vδ1 T-cell receptor transcripts showed diversity and were different for each individual. Vδ1 in the IELs were rearranged to Jδ1 and Vδ1 in the PBLs were rearranged not only to Jδ1 but also to Jδ3. Vδ1 sequences in the IELs in the nasal mucosa showed no overlap with those in the PBLs. These results indicate that γ/δ T cells in the nose are organ specific lymphocytes.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1046/j.1365-3083.2000.00784.x
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