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  • 1
    Electronic Resource
    Electronic Resource
    047 
Allogeneic Transplantation of Genetically Modified Primate Embryonic Stem Cells (2004)
    Oxford, UK; Malden, USA : Blackwell Science Inc
    Wound repair and regeneration 12 (2004), S. 0 
    Details
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Aim: To examine the efficacy and safety of human embryonic stem (ES) cell-based therapies, allogeneic transplantation of monkey ES cells would be useful. We transplanted genetically marked monkey ES cells into the allogeneic fetus. Methods and Results: Cynomolgus ES cells were transduced once using a simian immunodeficiency virus-based lentivirus vector encoding the GFP gene driven by the CMV promoter at 1, 10 and 100 transducing units per cell. Five days posttransduction, 60, 80 and 90% of the cells expressed GFP, respectively, and the expression levels were stable for 5 months. GFP expression was still observed after embryoid-body formation. The gene-marked ES cells were transplanted into the cynomolgus fetus in the abdominal cavity (n = 2) or liver (n = 1) after the first trimester. The fetuses were delivered 1 month posttransplantation. Transplanted cell progeny were detected (∼1%) in multiple tissues by quantitative PCR and in situ PCR of the GFP sequence. No teratoma was found in the tissues. Conclusions: Cynomolgus ES cells can be engrafted in the allogeneic fetus. We are now trying to transplant cynomolgus ES cells differentiated to neural or hematopoietic lineage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1067-1927.2004.abstractbi.x
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  • 2
    Electronic Resource
    Electronic Resource
    HA1077: A Novel Intracellular Calcium Antagonist. 1. Pharmacology (1996)
    Oxford, UK : Blackwell Publishing Ltd
    CNS drug reviews 2 (1996), S. 0 
    Details
    ISSN: 1527-3458
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1527-3458.1996.tb00295.x
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  • 3
    Electronic Resource
    Electronic Resource
    Open Wound Integrated Regenerative Therapy with Topical bFGF Trafermin for Deep Soft Tissue Defects with Bone Exposure (2005)
    Oxford, UK; Malden, USA : Blackwell Science Inc
    Wound repair and regeneration 13 (2005), S. 0 
    Details
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Aim:  Recently advocated concept of wound bed preparation (WBP) has systematically concreted the critical components of wound care, including debridement, bacterial balance, and management of exudate. We succeed in experiences by topical pharmaceutical therapy combined with WBP. We aimed to demonstrate availability of the wound management.Methods:  Since 1999, 18 exteriorized-bone patients with deep soft tissue defects were treated with this treatment. Our procedure was composed of four steps; 1) Resection or debridement, 2) Wound cleansing (soaking foot and hand in the hot bath with antibacterial carbonated agent), 3) Topical medication: basic fibroblast growth factor (Trafermin; KAKEN, Tokyo) and bucladecine sodium (Actocin ointment; Daiichi Pharmaceu. Co. Ltd, Tokyo), and 4) Dressing.Results:  The average time for whole coverage of the exposed-bone area with healthy granulation was 23 ±  13( median;19, range; 7–47) days after surgical treatment (the 1st step). The average time for complete epithelization was 72 ± 42 (55, 26–150) days in the patients who had not undergone skin graft. For minor amputations of diabetic feet the healing time until complete epithelization was 65 ± 46 (44, 26–150) days on an average.Conclusions:  We concluded that the integrated approach mobilizing commercial available products for wound healing under optimally prepared environment offers advantage of facilitating the efficacy of exogenous therapeutic measures on condition that endogenous regeneration has been accelerated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1067-1927.2005.130116al.x
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  • 4
    Electronic Resource
    Electronic Resource
    Kinetics of reactions in solutions under pressure. XXXI. Ionization constants and volumes of highly hindered pyridines in methanol (1975)
    s.l. : American Chemical Society
    The @journal of organic chemistry 40 (1975), S. 1179-1181 
    Details
    ISSN: 1520-6904
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/jo00896a044
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  • 5
    Electronic Resource
    Electronic Resource
    Interface analysis of Y-Ba-Cu-O films on Al-coated Si substrates (1989)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 54 (1989), S. 1275-1277 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Y-Ba-Cu-O films were deposited on Al-coated Si substrates by the plasma-spray method. The Al buffer layer appears to be effective in yielding crack-free adhesive Y-Ba-Cu-O films. Resistance measurements indicate that the films exhibit a superconducting phase below 90 K. Results of x-ray microanalysis and x-ray photoelectron spectroscopy confirm that the Al buffer forms an Al2O3 layer and prevents precipitation of Cu at the film/substrate interface.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.101489
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  • 6
    Electronic Resource
    Electronic Resource
    Structural characterization of Si1−xGex alloy layers grown by molecular beam epitaxy on Si(001) substrates (2000)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 87 (2000), S. 8759-8765 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Residual strain, surface roughness, and dislocations of Si1−xGex alloy layers grown by molecular beam epitaxy on Si(001) substrates at 550 °C have been characterized by x-ray diffraction, atomic force microscopy, and cross-sectional transmission electron microscopy (XTEM). Two kinds of samples were grown. One is a series of Si1−xGex alloy layers with x≤0.3 and a thickness of 5000 Å directly grown on Si(001) substrates, and the other is a series of Si0.7Ge0.3 alloy layers with a thickness of 2000 Å grown on Si(001) via compositionally graded Si1−yGey buffer layers (0≤y≤x). The Ge grading rate gr in the buffer layer, defined by gr% Ge/μm, ranged from 22 to 76. In the case of direct growth, the surface morphology changes from a wavy ripple pattern to a cross-hatch pattern with increase in x, and islandlike patterns appear at x=0.3. The residual strain decreases with increase in x, whereas the surface roughness increases with x. In the case of Si0.7Ge0.3 alloy layers grown with buffer layers, the surfaces of all samples display cross-hatch pattern. The surface roughness is highest for a grading rate of about 35, and it decreases for both lower and higher grading rates. The residual strain also shows a similar dependence on the grading rate. XTEM images are correlated to the residual strain and surface roughness. © 2000 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.373607
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  • 7
    Electronic Resource
    Electronic Resource
    CYCLIC NUCLEOTIDE PHOSPHODIESTERASE OF HUMAN CEREBROSPINAL FLUID (1975)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 25 (1975), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract— Cyclic 3′,5′-AMP (cAMP) and cyclic 3′,5′–GMP (cGMP) phosphodiesterase activities were found in human cerebrospinal fluid (CSF) using low substrate concentration (0.4μM). More rapid hydrolysis of cGMP than that of cAMP was observed in human CSF. However, cGMP hydrolytic activity of CSF was very much lower (0.3 pmol/min/ml CSF) than that of human cerebral cortex (33.7 nmol/min/g wet cortex). The pH optimum was found to be 8.0 (cGMP phosphodiesterase) and 7.5 (cAMP phosphodiesterase). The maximum stimulation of both cAMP and cGMP phosphodiesterase was achieved at 4 mM-MgCl2. Cyclic AMP had relatively little effect on the hydrolysis of cGMP in CSF and the cortex, while cGMP inhibited hydrolysis of cAMP in both tissues. Snake venom was found to stimulate cAMP and cGMP phosphodiesterase activity of CSF, by 60% and 110% respectively. This stimulation by snake venom was also observed in the cortex phosphodiesterase, but was not observed in human plasma or thyroid phosphodiesterase. When CSF was applied to Sepharose 6B column, cGMP phosphodiesterase was separated into three different molecular forms. A plot of activity against substrate concentration using peak I (largest molecular size) revealed a high affinity (Km= 2.6μM) and a low affinity (Km= 100μM) for cAMP suggesting the existence of at least two molecular forms of the enzyme. On the other hand, using a cGMP as substrate the only one Km value (1.90 μm) was obtained. These Km values of CSF enzymes described above were close to those obtained from human cerebral cortex preparations. The enzyme under peak I corresponded to the cortex enzyme when judged from its molecular size and stimulation by snake venom. It seems likely from our results that at least a part of CSF phosphodiesterase originates from the central nervous system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1975.tb07692.x
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  • 8
    Electronic Resource
    Electronic Resource
    Development of high-strength, high-conductivity Cu–Ag alloys for high-field pulsed magnet use (1991)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 59 (1991), S. 2965-2967 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: A wire-conductor fabrication method has been developed for Cu–Ag alloys containing 2–60 at. % Ag where high strength and high conductivity conductors are obtained by cold working combined with intermediate heat treatment. The intermediate heat treatment is repeated 3–4 times at 350–450 °C for 1–2 h at appropriate stages of reduction of area. The optimized Cu-16 at. % Ag alloy wire with 99% reduction of area showed a tensile strength of 1000 MPa and an electrical conductivity of 80% IACS at room temperature. This suggests that the wires fabricated may be very promising for high-field pulsed magnet use.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.105813
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  • 9
    Electronic Resource
    Electronic Resource
    Observation of ultrafast all-optical modulation based on intersubband transition in n-doped quantum wells by using free electron laser (1996)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 69 (1996), S. 4136-4138 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: A picosecond-range all-optical modulation based on the nonlinear interaction between interband- and intersubband-resonant light in GaAs/AlGaAs multiple quantum wells was demonstrated. A free electron laser (FEL) with a very short pulse width (〈10 ps) was used for the intersubband excitation. The dependence of the modulation depth on the wavelength of the intersubband-resonant light was investigated by utilizing the wide tunability of the FEL. The result is in good agreement with the intersubband absorption spectrum of the multiple quantum wells measured by FT-IR spectroscopy, which indicates that the observed modulation is indeed based on the intersubband transition induced by the FEL. © 1996 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.117838
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  • 10
    Electronic Resource
    Electronic Resource
    Pump-probe measurement of ultrafast all-optical modulation based on intersubband transition in n-doped quantum wells (2000)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 77 (2000), S. 19-21 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Modulation of interband-resonant light (∼800 nm) by intersubband-resonant light (5–7 μm) was investigated in n-doped AlGaAs/GaAs multiple quantum wells by a two-color femtosecond pump-probe technique. Modulation with a recovery time of ∼1 ps is observed in a plainer-type modulation device at room temperature. The modulation of interband absorption coefficient is ∼1000 cm−1 when the energy density of the intersubband light pulse is ∼4 fJ/μm2. The modulation efficiency indicates that 99% modulation can be achieved with a control pulse energy of ∼1 pJ when a conventional waveguide-type device structure is utilized. The mechanism which determines the modulation speed is discussed in terms of carrier relaxation process. It is shown that the modulation speed is mainly determined by the inter- and intrasubband relaxation times, where the latter is influenced by hot phonon effects. © 2000 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.126863
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