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  • 1
    Electronic Resource
    Electronic Resource
    Errors in genomics and proteomics (2002)
    [s.l.] : Nature Publishing Group
    Nature biotechnology 20 (2002), S. 19-19 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] To the editor Large-scale studies of gene expression on the RNA, protein, and/or metabolite level should greatly help to understand complex biological processes. However, it now becomes apparent that the correlation between mRNA and protein levels is remarkably and unexpectedly low; for example, ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt0102-19b
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  • 2
    Electronic Resource
    Electronic Resource
    The roots of nodulins (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 79 (1990), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Nodulin gene expression is an integral and highly specific part of the formation of nitrogen-fixing nodules on the roots of leguminous plants. Dependent on the time of expression during root nodule development, nodulin genes can be divided into early and late nodulin genes. A brief overview of the functions assigned to early and late nodulins is presented. We hypothesize that nodulin genes originate from regular plant genes that evolved to fit the regulatory and/or physiological constraints of symbiotic nitrogen fixation. Data on nodulins and nodulin genes, nodulation taxonomy and nodule development are evaluated in the light of this hypothesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1990.tb06760.x
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  • 3
    Electronic Resource
    Electronic Resource
    Isolation of High Quality DNA and RNA from Leaves of the Carnivorous Plant Drosera rotundifolia (1999)
    Springer
    Plant molecular biology reporter 17 (1999), S. 269-277 
    Details
    ISSN: 1572-9818
    Keywords: carnivorous plants ; CTAB (hexadecyltrimethylammoniumbromide) ; DNA isolation ; Drosera ; RNA isolation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Drosera rotundifolia belongs to the family of the sundews, a large group of carnivorous plants that carry stalked glands on the upper leaf surface to attract, trap and digest insects for food. Therefore, such plants can live in relatively poor ecosystems. They are frequently used as medicinal herbs and have various other interesting characteristics associated with them. In attempts to evaluate the gene pool of these plants, we experienced that many published protocols for nucleic acid isolation failed to yield DNA and RNA of sufficient quality for analysis. Therefore, we have developed CTAB (hexadecyltrimethylammoniumbromide)-based extraction protocols for the routine isolation of high-quality DNA and RNA from small amounts of in vitro-grown Drosera rotundifolia leaves. The methods developed are simple, fast and effective. The obtained DNA could be analyzed by PCR, restriction endonucleases and DNA gel blotting, and the obtained RNA was of sufficient quality for RT-PCR and RNA gel blotting.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007627509824
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  • 4
    Electronic Resource
    Electronic Resource
    High efficiency Agrobacterium-mediated gene transfer to flax (1994)
    Springer
    Plant cell reports 13 (1994), S. 282-285 
    Details
    ISSN: 1432-203X
    Keywords: Flax (Linum usitatissimum L.) ; Transformation ; Agrobacterium tumefaciens ; ß-Glucuronidase ; Transgene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using an Agrobacterium tumefaciens binary vector (pAL4404, pBI131), we have demonstrated the transfer of the β-glucuronidase gene into the flax (Linum usitatissimum L.) cultivar Glenelg after selection for kanamycin resistance. The transformed lines were obtained by inoculation and subsequent regeneration of hypocotyl segments. The callus that formed on the cut surfaces of the hypocotyl segments was isolated three weeks after infection and was subsequently subcultured to yield shoots. This procedure generated a large number of transgenic shoots over a relatively short period of time. The transformation efficiencies obtained were the highest reported so far for this plant species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233320
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  • 5
    Electronic Resource
    Electronic Resource
    Activity of the promoter of the Lhca3.St.1 gene, encoding the potato apoprotein 2 of the light-harvesting complex of Photosystem I, in transgenic potato and tobacco plants (1993)
    Springer
    Plant molecular biology 23 (1993), S. 605-612 
    Details
    ISSN: 1573-5028
    Keywords: chlorophylla/b-binding proteins ; light-harvesting complex ; Photosystem I ; promoter analysis ; transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have isolated cDNA and genomic clones for the potato (Solanum tuberosum) apoprotein 2 of the light harvesting complex of Photosystem I, designated Lhca3.St.1. The protein shows all characteristics of the family of chlorophyll a/b-binding proteins. Potato Lhca3.1 gene expression occurs predominantly in leaves, and is transcriptionally regulated by light. One gene copy is present per haploid genome. The sequence of the 5′ upstream region was determined. Most boxes identified in the promoter sequences of genes whose expression is light-regulated recur in the Lhca3.St.1 sequence. Functional analyses of the Lhca3.St.1 promoter and two deletion derivatives in transgenic potato transformed with a promoter-GUS fusion show high promoter activity in leaves and other green parts of the plant, which depends on light. Activity is absent in roots and potato tubers. The 500 bp promoter fragment is as active as the full 2.0 kb sequence, showing that all regulatory elements are present on the smallest deletion derivative. In transgenic tobacco (Nicotiana tabacum) plants carrying the largest promoter derivative a similar distribution of activity is found. Promoter activity is not restricted to the phloem, but also prominent in the xylem of the young stem, which contrasts with promoters of other photosynthesis-associated genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00019307
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  • 6
    Electronic Resource
    Electronic Resource
    Isolation and analysis of cDNA encoding the 33 kDa precursor protein of the oxygen-evolving complex of potato (1991)
    Springer
    Plant molecular biology 17 (1991), S. 157-160 
    Details
    ISSN: 1573-5028
    Keywords: gene expression ; photosystem II ; Solanum tuberosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00036821
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  • 7
    Electronic Resource
    Electronic Resource
    Analysis of the region in between two closely linked patatin genes: class II promoter activity in tuber, root and leaf (1992)
    Springer
    Plant molecular biology 20 (1992), S. 683-694 
    Details
    ISSN: 1573-5028
    Keywords: patatin class II gene ; Solanum tuberosum ; transformation ; transgenic potato ; tuber-specific gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract From a potato genomic library a phage lambda clone was isolated that carried nucleotide sequences of two patatin genes, thus demonstrating a close physical linkage between these two members of the patatin gene family. Sequence and restriction analysis showed the genes to be oriented in tandem. The more upstream gene was a pseudogene truncated at the 3′ end, whereas the downstream gene was a class II patatin gene. In addition to a 208 bp fragment also present in patatin class I promoters, the region in between both genes contained various direct repeats also found in other patatin genes. To study the promoter activity of this intergenic region, a 2.78 kb fragment was transcriptionally fused to the β-glucuronidase gene and reintroduced into potato cultivar Bintje. Histochemical analysis revealed expression in the outermost layer of cells of the cortex, in the tuber phellogen, in or around the root vascular system, and also in the abaxial phloem layer of the vascular bundle in leaves.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00046453
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  • 8
    Electronic Resource
    Electronic Resource
    pBINPLUS: An improved plant transformation vector based on pBIN19 (1995)
    Springer
    Transgenic research 4 (1995), S. 288-290 
    Details
    ISSN: 1573-9368
    Keywords: transformation vector ; pBIN19 ; transgenic plants ; T-DNA ; Agrobacterium tumefaciens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We describe the construction of a new plant transformation vector, pBINPLUS, based on the popular pBIN19 vector. Improvements over pBIN19 include location of the selectable marker gene at the left T-DNA border, a higher copy number inE. coli, and two rare restriction sites around the multiple cloning site for easier cloning and analysis of T-DNA insertions in plant genomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01969123
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  • 9
    Electronic Resource
    Electronic Resource
    Review: Biosafety of E. coli β-glucuronidase (GUS) in plants (1998)
    Springer
    Transgenic research 7 (1998), S. 157-163 
    Details
    ISSN: 1573-9368
    Keywords: biosafety ; Escherichia coli ; familiarity ; genetic modification ; β-glucuronidase (GUS) ; substantial equivalence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The β-glucuronidase (GUS) gene is to date the most frequently used reporter gene in plants. Marketing of crops containing this gene requires prior evaluation of their biosafety. To aid such evaluations of the GUS gene, irrespective of the plant into which the gene has been introduced, the ecological and toxicological aspects of the gene and gene product have been examined. GUS activity is found in many bacterial species, is common in all tissues of vertebrates and is also present in organisms of various invertebrate taxa. The transgenic GUS originates from the enterobacterial species Escherichia coli that is widespread in the vertebrate intestine, and in soil and water ecosystems. Any GUS activity added to the ecosystem through genetically modified plants will be of no or minor influence. Selective advantages to genetically modified plants that posses and express the E. coli GUS transgene are unlikely. No increase of weediness of E. coli GUS expressing crop plants, or wild relatives that might have received the transgene through outcrossing, is expected. Since E. coli GUS naturally occurs ubiquitously in the digestive tract of consumers, its presence in food and feed from genetically modified plants is unlikely to cause any harm. E. coli GUS in genetically modified plants and their products can be regarded as safe for the environment and consumers
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008832711805
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  • 10
    Electronic Resource
    Electronic Resource
    Hybridization of radish (Raphanus sativus L.) and oilseed rape (Brassica napus L.) through a flower-culture method (1995)
    Springer
    Euphytica 83 (1995), S. 159-168 
    Details
    ISSN: 1573-5060
    Keywords: Brassica napus ; flower-culture method ; intergeneric cross ; oilseed rape ; radish ; Raphanus sativus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Hybridization between radish and oilseed rape has been cumbersome, requiring elaborate embryo rescue techniques. With a modified flower culture method, we have achieved successful hybridization between radish and (transgenic) oilseed rape without the laborious and technically demandingin vitro ovule or embryo rescue techniques. The hybrid nature of the intergeneric hybrids was demonstrated using morphological traits, and DNA analyses. The described method will facilitate the generation ofRaphanobrassica hybrids useful for biosafety studies of the potential for transgenes to spread in weedyCruciferae as well as for breeding programs aimed at introducing useful radish genes, e.g. nematode resistance genes, into oilseed rape.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01678044
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