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  • 1
    Electronic Resource
    Electronic Resource
    Dopamine Release via Protein Kinase C Activation in the Fish Retina (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 54 (1990), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Calcium-dependent phospholipid-sensitive protein kinase [protein kinase C (PKC)] was partially purified from the carp (Cyprinus carpio) retina through DE 52 ion exchange and Cellulofine gel filtration chromatography. The phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA) activated PKC in the nanomolar range. A major 38-kDa protein in the retinal supernatants (105,000 g) was phosphorylated in vitro by PKC during a short period (3 min). Other phosphoproteins also appeared during a further prolonged period (〉15 min). Rod-bipolar and dopamine (DA) interplexiform cells in the fish retina were immunoreactive to a monoclonal antibody to PKC (α/β-subtype). The PKC antibody recognized a 78-kDa native PKC enzyme by means of an immunoblotting method. Subsequently, the effects of two kinds of PKC activators were investigated on [3H]DA release from retinal cell fractions containing DA cells that had been preloaded with [3H]DA. A phorbol ester (TPA) induced a calcium- and dose-dependent [3H]DA release during a short period (2 min), with the minimal effective dose being 1 nM. Other phorbols having no tumor-promoting activity, such as 4β-phorbol and 4α-phorbol 12, 13-didecanoate, were ineffective on [3H]DA release. A synthetic diacylglycerol [1-oleoyl-2-acetylglycerol (OAG)], which is an endogenous PKC activator, was also able to induce a significant release of [3H]DA. Furthermore, TPA was found to release endogenous DA from isolated fish retina by a highly sensitive HPLC with electrochemical detection method. The OAG- or TPA-induced [3H]DA or DA release was completely blocked by inhibitors of PKC, such as 1-(5-isoquinolinesulfonyl)-2-methylpiperazine (H7) and staurosporine. In contrast, release of [3H]glycine, γ-[3H]aminobutyric acid, and D-[3H]aspartic acid was never induced by TPA. Taken together, the present data indicate that TPA or OAG triggers a release of DA synaptically and that the induction is due to a PKC activation specifically in the membrane of DA cells in the fish retina. The functional role of PKC in rod-bipolar cells is unknown at present.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1990.tb04914.x
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  • 2
    Electronic Resource
    Electronic Resource
    Design of an instrument for far-infrared microspectroscopy using a synchrotron radiation source : Proceedings of the 4th international conference on synchrotron radiation instrumentation (1992)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 63 (1992), S. 1551-1554 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: A design of a microspectrophotometric system using a synchrotron radiation (SR) source is described. The system covers a wide spectral range of 50–13000 cm−1, being under construction at the UVSOR BL6B beamline in the Institute for Molecular Science. Preliminary experiments in the mid-infrared region (500–5000 cm−1) have qualitatively confirmed the theoretical calculation that the synchrotron radiation is more intense than a blackbody (T=1200 K) when a microspectrophotomeric technique is applied, which is due to natural collimation and high brilliance of SR source. The SR as an infrared source exhibits its advantage on measuring the spectra of small single crystals especially in the far-infrared region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1143017
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Wave breaking and absorption efficiency for short pulse p-polarized laser light in a very steep density gradient (1993)
    New York, NY : American Institute of Physics (AIP)
    Physics of Fluids 5 (1993), S. 564-570 
    Details
    ISSN: 1089-7666
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The nonlinear interaction of an ultrashort-pulse laser with a solid target is investigated by using a newly developed particle simulation code, which enables the analysis of laser–plasma interaction in the very steep density gradient in which the laser light penetrates into an overdense plasma. For an ultrashort laser pulse, the plasma density scale length Ld satisfies Ld≤r0 for an electron excursion length r0. Laser absorption and electron acceleration processes are proposed for the above situation. It is found that the wave breaking is the main mechanism of the electron acceleration and laser absorption, as is discussed in Brunel's paper [Phys. Rev. Lett. 59, 52 (1987)].
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.860542
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  • 4
    Electronic Resource
    Electronic Resource
    Mitochondrial DNA Restriction Fragment Length Polymorphisms in Malus Species (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 111 (1993), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Three apple (Malus×domestica) cultivars and 11 Malus accessions have been investigated by the probe hybridization method on their mitochondrial DNA (mtDNA). The gene probes used were: coxI, coxII, atpA, atp6, and atp9. Our results revealed enough variation to characterize ten mtDNA haplotypes among the Malus genotypes examined. The taxonomic and phylogenetic implications of mtDNA polymorphism are also discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1993.tb00623.x
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  • 5
    Electronic Resource
    Electronic Resource
    Eicosapentaenoic Acid and Apoplexy Influence of EPA Loading on SHRSP (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 598 (1990), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1990.tb42303.x
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  • 6
    Electronic Resource
    Electronic Resource
    Ambient pressure stimulates immortalized human aortic endothelial cells to increase DNA synthesis and matrix metalloproteinase 1 (tissue collagenase) production (1994)
    Springer
    Virchows Archiv 425 (1994), S. 385-390 
    Details
    ISSN: 1432-2307
    Keywords: Pressure ; Human aortic endothelial cells Matrix metalloproteinase 1 ; Angiogenesis Atherosclerosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In the present study, we investigated the effect of ambient pressure on [3H]-thymidine incorporation and on the production of matrix metalloproteinase 1 (tissue collagenase/proMMP-1) using human aortic endothelial cells immortalized with simian virus 40 (SE-1). Incubation of cells at ambient pressures of 50 and 100 mmHg for 24 h slightly increased [3H]-thymidine incorporation when directly compared with normal culture conditions. The amount of [3H]-thymidine incorporated in SE-1 reached a maximum at 150 mmHg, while a further increase in pressure to 200 mmHg decreased incorporation. The same ambient pressure slightly stimulated human aortic intimal smooth muscle cells (SMC) to increase [3H]-thymidine incorporation but not medial SMC. Immunoblot analysis also showed that ambient pressure, ranging from 50 to 200 mmHg, like 12-O-tetradecanoyl-phorbol-13-acetate stimulated SE-1 to produce proMMP-1, an effect not seen with either intimal or medial SMC. The amount of proMMP-1 produced also reached a maximum level at 150 mmHg. We postulate that human endothelial cells are ambient pressure sensitive and that relatively lower ambient pressures play an important role in the growth of endothelial cells, while higher pressures injure endothelial cells, resulting in the initiation of atherosclerosis. This cell line may prove useful in the investigation of both the physiological and pathological roles of blood pressure on endothelial cell function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00189576
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  • 7
    Electronic Resource
    Electronic Resource
    Organelle DNA polymorphism in apple cultivars and rootstocks (1992)
    Springer
    Theoretical and applied genetics 83 (1992), S. 963-967 
    Details
    ISSN: 1432-2242
    Keywords: Apple ; Chloroplast DNA ; Mitochondrial DNA ; RFLP ; Cytoplasmic diversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Restriction fragment length polymorphisms (RFLPs) have been used to detect chloroplast (cp) and mitochondrial (mt) DNA variation among 18 apple cultivars and three rootstocks. The distribution of RFLP patterns allowed the assignment of these genotypes into three groups of cytoplasmic relatedness. Our results also demonstrate maternal inheritance of cp- and mtDNAs in apple. Thus, the organelle DNA assay provides a convenient and reliable method to assess cytoplasmic diversity within the apple germ-plasm collection and to trace the maternal lineages involved in the evolution of apple.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232957
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  • 8
    Electronic Resource
    Electronic Resource
    Presence of two different fibril subtypes in the Pick body: an immunoelectron microscopic study (1990)
    Springer
    Acta neuropathologica 81 (1990), S. 125-129 
    Details
    ISSN: 1432-0533
    Keywords: Pick body ; Tau protein ; Straight fibrils ; Constricted fibrils ; Immunoelectron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A study of Pick bodies (PBs) was performed using immunohistochemical, ultrastructural and immunoelectron microscopic techniques. Ultrastructurally, the PBs in the granular neurons of the dentate fascia of Ammon's horn were composed of randomly distributed straight fibrils (14.5±2.1 nm in diameter), intermingled with a few constricted fibrils. The constricted fibrils had a periodicity of 157±11 nm, and were 28.7±3.3 nm wide at their widest point midway between the constrictions and 15.3±3.2 nm wide at the point constriction. A few straight fibrils approximately 15 nm in diameter appeared to morphologically transform into fibrils with constrictions at approximately 160-nm intervals. Immunoelectron microscopy revealed that the straight fibrils, the constricted fibrils, and the transitional form (straight fibrils linked with constricted fibrils) had the same immunoreactivity to anti-tau antiserum. The two different subtypes of Pick fibrils, i.e., the straight fibrils and the constricted fibrils, have interchangeable appearances and a common pathomechanism is suggested to underlie the formation of these two subtypes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00334500
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  • 9
    Electronic Resource
    Electronic Resource
    Demonstration of neurofibrillary tangles and neuropil thread-like structures in spinal cord white matter in parkinsonism-dementia complex on Guam and in Guamanian amyotrophic lateral sclerosis (1994)
    Springer
    Acta neuropathologica 88 (1994), S. 180-184 
    Details
    ISSN: 1432-0533
    Keywords: Neurofibrillary tangle ; Neuropil thread ; Spinal cord white matter ; Parkinsonism-dementia complex on Guam ; Guamanian amyotrophic lateral sclerosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This report concerns the demonstration and distribution of neurofibrillary tangles (NFTs), immunoreactive neuropil thread-like structures and dots in the spinal cord gray and white matter of six patients with parkinsonism-dementia complex on Guam and five patients with Guamanian amyotrophic lateral sclerosis (ALS). A monoclonal antibody to Alzheimer NFTs was used. NFTs were found in the spinal cord gray matter and white matter and all patients had immunoreactive neuropil thread-like structures and dots in the gray matter as well as in the white matter. They were particularly numerous in the lateral funiculus of patients with Guamanian ALS.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00294512
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  • 10
    Electronic Resource
    Electronic Resource
    Divergent effects of hyperosmolality on stress-response (heat shock) protein expression in cultured human tumor cells: an immunocytochemical study (1994)
    Springer
    Cellular and molecular life sciences 50 (1994), S. 479-482 
    Details
    ISSN: 1420-9071
    Keywords: Stress-response proteins ; heat shock proteins ; hyperosmolality ; human tumor cells ; MCF-7 ; HeLa S3 ; immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Exposing cells to adverse conditions usually elicits expression of stress-response (heat shock) proteins (srp). Here we show that hyperosmolar growth conditions do not uniformly affect srp expression in MCF-7 and HeLa S3 cells, derived from carcinoma of the breast and cervix, respectively. Thus, whereas srp 27 expression was increased in MCF-7, but not in HeLa S3, the opposite was the case with srp 72. On the other hand, hyperosmolality did not induce αB-crystallin or ubiquitin in either cell line. These findings show that srp expression by the human tumor cells studied is non-coordinate, suggesting that each srp is independently modulated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01920751
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