ISSN:
0138-4988
Keywords:
Life Sciences
;
Life Sciences (general)
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Potato starch hydrolysis products were analyzed for glucose, maltose, higher oligomeric carbohydrates (to a degree of polymerization, dp 11) and 5-hydroxymethylfurfural (HMF) using high-performance liquid chromatography.For quick qualitative and quantitative analyses short glass columns [12] of high efficiency were useful.The hydrolyses were carried out by means of enzymes (e.g. α- and β-amylase) or mineral acids. For the acid degradation procedures hydrochloric acid, sulfuric acid and phosphoric acid of different concentrations (0.1…2.0 N) during times ranging from 5 to 60 min at temperatures ranging from 100 to 140°C were used.Maximum glucose contents (163.4 g/l and 169.3 g/l) were found after 40 to 50 min of hydrolysis in 0.1 N hydrochloric acid heated to 120°C. These values are equivalent to 78.9% or 81.7% glucose yield referred to the initial potato starch amount, respectively. The calculated HMF concentrations were 140 and 180 mg/l.
Additional Material:
8 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/abio.370080417
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