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  • 1
    Digitale Medien
    Digitale Medien
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 68 (1996), S. 3719-3721 
    ISSN: 1077-3118
    Quelle: AIP Digital Archive
    Thema: Physik
    Notizen: Experimental results on pulsed corona and dielectric-barrier discharge processing of very dilute concentrations of NO in N2 are presented. These NO reduction experiments measure the G value for electron-impact dissociation of N2 and are used to infer the effective electron mean energy in an N2 discharge plasma at atmospheric pressure. The data have been obtained from three different laboratories using widely differing electrode structures, voltage wave forms, power measurements, and chemical analyses. The NO reduction yields from the discharge reactors tested are all similar, corresponding to an electron mean energy of 4.0±0.5 eV. © 1996 American Institute of Physics.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 150 (2004), S. 0 
    ISSN: 1365-2133
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Background  It is well accepted that ultraviolet radiation from the sun can induce and promote growth of skin tumours. Skin cancer develops as a consequence of multiple genetic hits, where an initial, important step includes proliferation of cells susceptible to malignant transformation. Foci of morphologically normal epidermal keratinocytes overexpressing p53 protein are common in chronically sun-exposed skin. Such foci have previously been shown to represent expanding clones of p53-mutated keratinocytes. Although several characteristics concerning epidermal p53 clones remain to be resolved, an important role in skin carcinogenesis is anticipated. The density of epidermal p53 clones in human skin is largely unknown.Objectives  To compare the occurrence of epidermal p53 clones in skin surrounding cancers with that in skin surrounding benign melanocytic naevi. To assess the influence of age on frequency and size of epidermal p53 clones in human facial skin.Methods  We have analysed the number and sizes of epidermal p53 clones in skin specimens from patients with squamous cell carcinoma (SCC), basal cell carcinoma (BCC) and benign melanocytic naevi. Cases included normal facial skin from four different age groups. Tissue sections were immunohistochemically stained and the presence of p53 clones was recorded. Approximately 1·4 m of epidermis from a total of 112 biopsies was analysed.Results  We found 128 epidermal p53 clones in biopsy specimens from 112 patients. The results showed that the number and size of p53 clones increase with age. In normal skin adjacent to SCC p53 clones were significantly more numerous and greater in size in comparison with those in normal skin both adjacent to benign naevi and adjacent to BCC. Interestingly, normal skin in the close vicinity of BCC and melanocytic naevi showed similar results regarding both number and size of epidermal p53 clones.Conclusions  Our findings suggest a connection between development of epidermal p53 clones and SCC.
    Materialart: Digitale Medien
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  • 3
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 733 (1994), S. 0 
    ISSN: 1749-6632
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Allgemeine Naturwissenschaft
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 343 (1980), S. 0 
    ISSN: 1749-6632
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Allgemeine Naturwissenschaft
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 78 (1977), S. 1383-1389 
    ISSN: 0006-291X
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie , Chemie und Pharmazie , Physik
    Materialart: Digitale Medien
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  • 6
    ISSN: 1432-2277
    Schlagwort(e): Glioblastoma, liver transplantation ; Liver transplantation, glioblastoma ; Malignancy, donor related, liver transplantation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The transmission of donor-related malignancies by organ transplantation is a rather rare event. There has only been one report on the development of a brain tumor metastasis in liver transplantation. From September 1988 to January 1993, 342 donor hepatectomies with subsequent transplantation were performed at our center. The main donor diagnoses included subarachnoidal bleeding (n=128; 37.4%), isolated head injury (n=114; 33.3%), multiple injuries (n=55; 16.1%), primary cerebral neoplasia (n=13; 3.8%), and other (n=32; 9.4%). Primary cerebral neoplasia included glioblastoma (n=4), meningioma (n=3), astrocytoma (n=2), angioma (n=2), neurocytoma (n=1), and ependymoma (n=1). In the group of donors suffering from primary cerebral neoplasia, procured organs other than the liver included kidneys (n=20), combined kidneys and pancreata (n=1), pancreata (n=2) hearts (n=8), combined hearts and lungs (n=1), and single lungs (n=1). Follow-up of the respective graft recipients ranged from 28 to 68 months (median 43 months). Recurrent malignancy was observed once, in a liver graft recipient. The donor, a 48-year-old female, had undergone surgical resection of an intracerebral multiform glioblastoma and died 4 months later of a relapse in the brain stem. The 28-year-old female recipient had undergone transplantation for an autoimmune-hepatitic cirrhosis. Four months later, histopathological examination of an intraperitoneal and intrahepatic mass revealed a poorly differentiated, small-cell pleomorphic cancer, identified as a glioma metastasis by S100-and glial fibrillary acidic protein immunohistochemical staining. The patient died 6 months post-transplantation. On autopsy, no further neoplastic lesions were detected. Our review adds a second reported case of a liver graft-transmitted brain tumor to the literature and the fourth donor-related malignancy after hepatic transplantation in general.
    Materialart: Digitale Medien
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  • 7
    ISSN: 1432-2323
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract. In gallbladder carcinoma, studies on the prime target of genetic alterations and gene therapy in human gallbladder malignancies, the p53 tumor suppressor gene, have been focusing on this gene’s immunohistochemical detection. From November 1991 to October 1993, seven patients suffering from gallbladder carcinoma underwent surgical resection. Cancerous and normal liver tissues were obtained immediately after surgery, snap-frozen in liquid nitrogen, and stored at −80°C for immunohistochemistry and DNA isolation. Exons 5, 6, 7, and 8 of the p53 gene were completely sequenced following polymerase chain reaction (PCR) amplification of a 1574-bp fragment. Missense mutations were detected in the cancerous tissues of two patients: one transition each on codons 134 (Phe→Leu) and 146 (Trp→Arg). Immunohistochemical p53 staining was positive in the latter patient only. This is the first report on sequence analysis and mutagenesis of the p53 gene in Caucasian patients with gallbladder cancer. Both mutations were transitions and seem to represent a rather rare event. The possible impact of p53 mutagenesis on gallbladder tumorigenesis requires evaluation in larger studies.
    Materialart: Digitale Medien
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  • 8
    ISSN: 1432-2323
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Résumé Pour traiter le cancer de la partie supérieure de l'arbre biliaire la stratégie actuelle des auteurs est de procéder à l'exérèse radicale de la tumeur ou de pratiquer une transplantation lorsque la tumeur ne peut Être réséquée dès lors qu'il n'y a pas d'extension extra-hépatique du processus tumoral. L'exérèse de la tumeur est effectuée par résection isolée du hile biliaire ou résection associée de la lésion et d'un segment du foie; cette dernière méthode qui s'applique aux cancers plus étendus est recommandée car plus radicale. Leur conception repose sur leur expérience concernant 108 cas opérés de février 1975 à octobre 1986. Chez 10 malades aucune intervention radicale ou palliative ne put Être pratiquée en raison du stade avancé de la tumeur. Chez 30 patients: différentes opérations de drainage furent pratiquées. En revanche, 52 sujets subirent une exérèse: 25 une résection biliaire, 27 une résection du hile associée à une hépatectomie partielle; 28 de ces résections étant considérées comme opération palliative, 24 comme palliative. Seize malades qui présentaient une lésion inacessible à l'exérèse ont été traités par une transplantation hépatique mais 7 d'entre eux accusèrent ultérieurement une extension extra-hépatique du processus tumoral. Les temps de survie furent de 1 mois après laparotomie, 5 mois après intervention de drainage, 15 mois après résection, 23 mois après opération dite curative, 7 mois après opération dite palliative, 21 mois après transplantation chez 7 malades. En raison des résultats favorables chez les derniers malades, la transplantation hépatique constitue pour les auteurs l'ultime chance de traitement radical des patients qui relèveraient autrement d'une opération palliative de drainage du fait de l'importance de la tumeur.
    Kurzfassung: Resumen Nuestra estrategia actual en el tratamiento del adenocarcinoma de la porción proximal del canal biliar es la resección radical del tumor y, para los pacientes con tumores no resecables, la posibilidad de trasplante hepático si se ha demostrado que no hay crecimiento tumoral extrahepático. La resección tumoral es realizada mediante la resección del hilio solamente o combinada con hepatectomía parcial. Este Último procedimiento, que hace posible el tratamiento radical de los estados tumorales más avanzados y que eventualmente logra un mayor grado de radicalidad, es el recomendado. El concepto se fundamenta en la experiencia con 108 pacientes con carcinoma del canal biliar proximal operados entre febrero de 1975 y octubre de 1986. En 10 pacientes no fue posible realizar procedimiento alguno de tipo terapéutico o paliativo durante la laparotomía debido al avanzado estado del tumor. Diversos procedimientos de drenaje fueron ejecutados en 30 pacientes. Cincuenta y dos pacientes fueron sometidos a resección, 25 con resección del hilio solamente, 27 con resección combinada con resección parcial del hígado; 28 de las resecciones fueron clasificadas como curativas y 24 como paliativas; 16 pacientes con tumores no resecables reciberion trasplante hepático, y en 7 de ellos había crecimiento tumoral extrahepático en el momento del trasplante hepático. Las supervivencias medias fueron: laparotomía, 1 mes; procedimientos de drenaje, 5 meses; resección total, 15 meses; resección curativa, 23 meses; resección paliativa, 7 meses; trasplante hepático, 16 meses. Siete pacientes se hallan vivos a los 21 meses posttrasplante. Con base en los resultados favorables en el grupo más reciente de nuestros pacientes, el trasplante de hígado como la Última posibilidad de remoción del tumor en pacientes que no podrían ser tratados sino mediante procedimientos paliativos de drenaje, puede estar justificado.
    Notizen: Abstract In the treatment of adenocarcinoma of the proximal bile duct, our current strategy is to resect the tumor radically and to offer patients with unresectable tumors the chance of hepatic transplantation, if extrahepatic tumor growth is exluded. Tumor resection is performed by resection of the hilum alone or combined with partial hepatectomy. The latter procedure enables radical treatment of more advanced tumor stages and, eventually, a higher degree of radically is achieved, and is recommended. This concept is based on our experience with 108 patients with proximal bile duct carcinoma operated on between February, 1975 and October, 1986. In 10 patients, no therapeutic or palliative surgical procedure could be performed during laparotomy because of advanced tumor stage. In 30 patients, various drainage procedures were performed. Fifty-two patients underwent resection: 25 underwent resection of the hilum only, and 27 underwent resection of the hilum combined with partial liver resection. Twenty-eight of these resections were classified as curative and 24 as palliative. Sixteen patients with unresectable tumors had hepatic transplantation. In 7 of these patients, extrahepatic tumor growth was already present at the time of liver transplantation. Median survival times were: laparotomy only, 1 month; drainage procedures, 5 months; total resection, 15 months; curative resection, 23 months; palliative resection, 7 months; liver grafting, 16 months. Seven patients are alive up to 21 months posttransplantation. On the basis of favorable results in our more recent group of patients, liver grafting as the ultimate chance for tumor removal in patients otherwise treatable only by palliative drainage procedures may be justified.
    Materialart: Digitale Medien
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  • 9
    ISSN: 1432-2277
    Schlagwort(e): Key words Doppler sonography ; Acute rejection ; Chronic rejection ; Tacrolimus ; Kidney transplantation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The aim of the present study was to differentiate acute rejection, chronic rejection, and tacrolimus nephrotoxicity with color and power Doppler imaging of renal transplants. One hundred examinations were obtained from 45 patients. Pulsatility and resistive indices were calculated from color Doppler images. The grade of renal vascularization was quantified using computer-assisted pixel analysis in a rectangular region-of-interest. The percentage of vessel-covered renal parenchyma (POV) was calculated using a histogram that discriminated renal vessels from renal parenchyma via power Doppler images. Furthermore, the distance from the most peripherally located vessels to the renal capsule (PVD) was measured. A reduced POV K 55 % proved to be the best discriminator when chronic rejection was suspected (sensitivity 79 %, specificity 87 %). Tacrolimus nephrotoxicity showed not only a moderate elevation of the Doppler signal but also an increased PVD L 3.9 mm and a normal POV. We conclude that the evaluation of renal vessels by power Doppler images improves diagnostic accuracy for patients with renal allografts.
    Materialart: Digitale Medien
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  • 10
    ISSN: 1432-2277
    Schlagwort(e): Key words Liver transplantation ; Hepatitis B reinfection ; Portal vein thrombosis ; Recombinant tissue plasminogen activator lysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Portal vein thrombosis (PVT) is an infrequent complication following hepatic transplantation. However, deterioration of liver function and accompanying complications may be life threatening. Several attempts of surgical or percutaneous transhepatic procedures have been described. In some cases high dose fibrinolytic regimens have been successful. We describe the case of a male liver recipient with recurrent liver fibrosis due to hepatitis B reinfection and late portal vein thrombosis 45 months after transplantation. Complete recanalization was achieved using systemic low dose recombinant tissue plasminogen activator (rt-PA).
    Materialart: Digitale Medien
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