ISSN:
1432-072X
Schlagwort(e):
Methanothrix
;
Archaebacteria
;
Gluconeogenesis
;
Glycogen
;
Glycogen synthase
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract An intracellular glycogen was purified and characterized from the acetoclastic bacteria Methanothrix str. FE, its average chain length was about 13 glucose residues. Acetyl-CoA was shown to be synthesized by the action of acetate thiokinase; in addition pyruvate synthase, phosphoenolpyruvate synthetase and enzymes of gluconeogenesis were detected in cell extracts. For glycogen synthase activity, both adenosine diphosphate glucose and uridine diphosphate glucose were used as glycosyl donors, apparent K m were, respectively, 8 μM for ADPGlc and 625 μM for UDPGLe, at the opposite the V m were the same for both precursors. This was in accordance with competition experiments and strongly suggested that only one glucosyl transferase was involved and that ADPGlc was the physiological glycosyl donor in Methanothrix str. FE. In addition branching enzyme activity (α1-4-glucan-6-glucosyl transferase) was detected in cell extracts.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00410939
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