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  • 2015-2019
  • 1990-1994
  • 1975-1979
  • 1970-1974  (114)
  • 1930-1934
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  • 1830-1839
  • 1974  (114)
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  • Electron microscopy  (114)
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  • 2015-2019
  • 1990-1994
  • 1975-1979
  • 1970-1974  (114)
  • 1930-1934
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  • 101
    Electronic Resource
    Electronic Resource
    Similarities between Hassall's corpuscles of the human thymus and the epidermis (1974)
    Springer
    Cell & tissue research 151 (1974), S. 347-368 
    Details
    ISSN: 1432-0878
    Keywords: Human thymus ; Hassall's corpuscles ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Substrate-histochemical, enzyme-hystochemical and ultrastructural investigations were performed on thymic tissue from children, obtained in heart operations. β-Amylaseresistant, PAS-positive and Hale-positive substrates presumably neutral and acid mucosubstances, can be demonstrated in the central concentric lamellae of Hassall's corpuscles (HC). These lamellae also give positive reactions for sulphydryl groups and disulphide groups. Some flattened cell elements gave strong reactions for phospholipids, and small sudanophilic droplets, presumably neutral fats, are scattered throughout the HC. All investigated hydrolases and dehydrogenases either give no or only very weak reactions in the central part of progressive HC, but react strongly positive in their peripheral hypertrophic epithelial cells. In the central part of regressive HC, positive reactions for acid phosphatase and β-D-glucuronidase were recognized. These lysosomal enzymes may indicate degenerative processes. By electron microscopy progressive HC show central concentric lamellae with an amorphous matrix tightly filled with tonofilaments. They are surrounded by a thickened plasma membrane (200 Å), and do not contain nuclei. These central lamellae resemble the horny cells of the epidermis. The peripheral hypertrophic epithelial cells have pale nuclei with one or two nucleoli. Their cytoplasm contains numerous tonofibrils. These cells resemble stratum spinosum cells of the epidermis. In regressive HC the central concentric lamellae loose their intercellular contacts. The widened intercellular spaces are filled with cellular debris, and are invaded by macrophages. Similarities between the ultrastructure and the patterns of the histochemically investigated substrates and enzymes in human HC and epidermis are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224546
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  • 102
    Electronic Resource
    Electronic Resource
    Studies on the juxtaglomerular apparatus (1974)
    Springer
    Cell & tissue research 151 (1974), S. 471-480 
    Details
    ISSN: 1432-0878
    Keywords: Juxtaglomerular apparatus ; Rat ; Influence of deoxycorticosterone (DOC) ; Electron microscopy ; Morphometric analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die quantitativ-morphologische Analyse des juxtaglomerulären Apparates nach DOC-Behandlung zeigte, daß mit der Hemmung der Reninbildung eine Hypoplasie der epitheloiden Zellen des juxtaglomerulären Apparates einhergeht. Der Granulagehalt der juxtaglomerulären Zellen nahm ab; die Kern-Plasma-Relation änderte sich und die Leiomyofibrillen in den Epitheloidzellen nahmen zu. An den übrigen Anteilen des juxtaglomerulären Apparates, insbesondere der Macula densa, wurden keine morphologischen Veränderungen beobachtet. Die DOC-Behandlung führte darüber hinaus zu einer Bildung von Einschlüssen in den Podocyten, den Mesangiumzellen und den Zellen der Bowman-Membran. Die verschiedenen Möglichkeiten, über welche Faktoren die juxtaglomerulären Zellen nach DOC-Behandlung degranuliert werden, werden diskutiert. Das ultrastrukturelle Bild der Epitheloidzellen nach DOC-Behandlung entspricht deutlich einer gehemmten Sekretion, da das Ergastoplasma und der Golgi-Apparat ganz besonders stark zurückgebildet werden.
    Notes: Summary Quantitative morphological analysis of the Juxtaglomerular apparatus of rats treated with deoxycorticosterone (DOC) and salt revealed hypoplasia of the epithelioid cells as a concomitant of the inhibition of renin synthesis. The granular content of the Juxtaglomerular cells was significantly decreased, while the nucleus-to-cytoplasm ratio and the volume density of leiomyofilaments in the epithelioid cells increased. No morphological alterations were observed in the remaining constituents of the Juxtaglomerular apparatus, including the macula densa. DOC-treatment also induced the appearance of inclusions in the podocytes in the cells of the mesangium, and in the cells of Bowman's capsule. The possible mechanisms of degranulation of the Juxtaglomerular cells after treatment with DOC are discussed. The ultrastructural appearance of the epithelioid cells after treatment with DOC corresponds to an inhibition of secretion, since the rough endoplasmic reticulum and the Golgi apparatus undergo marked regressive changes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222992
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  • 103
    Electronic Resource
    Electronic Resource
    The reaggregation of neurons and their satellite cells in cultures of trypsin-dissociated spinal ganglia (1974)
    Springer
    Cell & tissue research 152 (1974), S. 175-183 
    Details
    ISSN: 1432-0878
    Keywords: Cell aggregation ; Spinal ganglia ; Cell cultures ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Dorsal root spinal ganglia of chick embryos were trypsinized and the cellular components isolated. Suspensions of these cells reaggregate. During this process satellite glial cells and neurons established intimate contacts. The reconstructed glial envelopes around the neurons are morphologically similar to the ones formed in the intact animal during development. The formation of the characteristic glial-neuronal relation is an example of specific cell recognition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224693
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  • 104
    Electronic Resource
    Electronic Resource
    The interaction of phagocytes and the large-sized parasite Cryptococcus neoformans: Cytochemical and ultrastructural study (1974)
    Springer
    Cell & tissue research 152 (1974), S. 165-174 
    Details
    ISSN: 1432-0878
    Keywords: Phagocytosis ; Large foreign bodies ; Cryptococcus neoformans ; Tissue culture ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The yeast Cryptococcus neoformans may develop under certain conditions a large polysaccharide capsule 50–100 μM in diameter and therefore cannot be phagocytosed by either polymorphonuclear cells (PMN's) or mononuclear phagocytes (MN's). The cellular defense mechanism — in various animals — against the yeast is composed by formation of ringlike structure of PMN's or MN's cells which surround the C. neoformans. Ring structures develop either in vivo or in vitro in tissue culture; destruction of the yeast occurs within 36–72 hours. Several hydrolases, such as acid phosphatase, β-glucuronidase and non-specific esterase were found to be released from the phagocytic cells into the enclosed yeast. Considerable reduction of NBT used as a marker for oxidative activity was observed in MN rings at contact regions of the MN cells and the yeast. Electron microscopic studies indicate that the phagocytic cells in the ring structure have many pseudopodes penetrating into the polysaccharide capsule of the yeast. Disintegration of the capsule was observed as well as phagocytosis of its material. A possible analogy between normal phagocytosis of small-sized bodies and the ring structure obtained when large bodies are involved is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224692
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  • 105
    Electronic Resource
    Electronic Resource
    Involution of the caudal musculature during metamorphosis in the ascidian, Botryllus schlosseri (1974)
    Springer
    Cell & tissue research 153 (1974), S. 293-305 
    Details
    ISSN: 1432-0878
    Keywords: Ascidia (Botryllus schlosseri) ; Metamorphosis ; Muscle tissue involution ; Phagocytes ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The caudal musculature of the free-swimming tadpole of the ascidian, B. schlosseri consists of cylindrical mononucleated cells connected in longitudinal rows flanking the axial notochord. During resorption of the larval tail, which is apparently induced by the contraction of the epidermis, muscle cells are dissociated and pushed into the body cavity where most of them are rapidly engulfed by phagocytes. In the initial stages of tail withdrawal muscle cells display surface alterations due to the disruption of intercellular junctions and disarrangement of myofibrils. Extensive degenerative changes, with shrinkage of mitochondria and disintegration of the contractile material are subsequently observed. Lysosomes and autophagic vacuoles are rarely seen and appear to play a secondary role in the degradation of the muscle cells, which occurs predominantly within the phagocytes. Myofilaments and myofibrils have never been observed within autophagic vacuoles. Clumps of muscle fragments and degenerated phagocytes undergo eventual dissolution in the blood lacunae, concomitantly with the differentiation of the young oozooid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00229160
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  • 106
    Electronic Resource
    Electronic Resource
    Adhesion plaques associated with the production of a daughter cell in Euglypha (Testacea; Potozoa) (1974)
    Springer
    Cell & tissue research 153 (1974), S. 261-268 
    Details
    ISSN: 1432-0878
    Keywords: Amoebae (Testacea) ; Siliceous plates ; Production ; Role of microtubules in fission ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Euglypha acanthophora and Euglypha strigosa, testate amoebae with siliceous shells, undergo binary fission producing daughter cells. The siliceous plates from which the shells are constructed are produced in the Golgi and perinuclear regions of the parent. At binary fission these pass along microtubule pathways and are manoeuvered into position in the daughter-cell by microtubule (20–25 nm) and microfilament (7–9 nm) systems. The latter in the form of adhesion plaques are instrumental in the coordination and deployment of the shell-plates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226614
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  • 107
    Electronic Resource
    Electronic Resource
    Transformations of hypothalamic arcuate neurons (1974)
    Springer
    Cell & tissue research 153 (1974), S. 497-515 
    Details
    ISSN: 1432-0878
    Keywords: Arcuate nucleus ; Hypothalamus ; Sexual cycle ; Ribbon-rolls ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Changes in subcellular structures of arcuate neurons correlated in a consistent way with stages of the estrous cycle of the rat. Associated with the rough endoplasmic reticulum short ribbons of moderately electron dense material appeared at metestrus and circular or elliptical bodies termed “ribbon-rolls” at diestrus and proestrus. Although present in proestrus, the ribbon-rolls were smaller at this stage. In a few neurons in diestrous females and in ovariectomized animals one to seven months before perfusion multiple large ribbon-rolls occupied much of the cytoplasm. Also, frequency of dense granules and lysosomes increased in diestrus. The significance of the ribbon-rolls and changes in other structures during the estrous cycle are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231543
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  • 108
    Electronic Resource
    Electronic Resource
    The ventricular system in neuroendocrine mechanisms (1974)
    Springer
    Cell & tissue research 154 (1974), S. 1-16 
    Details
    ISSN: 1432-0878
    Keywords: Ventricle ; Tanycytes ; Monoamine transport ; Autoradiography ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary This investigation has dealt with a light and electron microscopic autoradiographic analysis of the median eminence of the rat following intraventricular infusion of 3H-dopamine. This study has demonstrated that 3H-dopamine, once infused into the mammalian cerebral ventricular system, is rapidly and selectively absorbed by tanycytes and transported to the contact zone within 5 minutes after ventricular infusion. Certain axon terminals in the ependymal, hypendymal, and palisade-contact zones selectively sequester 3H-dopamine as do subpopulations of arcuate neurons. This picture of intense labelling of neurons and axon terminals is discussed with respect to monoaminergic re-uptake mechanisms. Uptake and transport of 3H-DA by tanycytes is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221067
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  • 109
    Electronic Resource
    Electronic Resource
    A new membrane organelle in developing amphibian neurones (1974)
    Springer
    Cell & tissue research 154 (1974), S. 103-108 
    Details
    ISSN: 1432-0878
    Keywords: Plasma membrane specialisations ; Developing neurons ; Xenopus laevis ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Membrane specialisations have been found on neurones in embryos of the clawed toad Xenopus laevis. The specialisations have been called dense membrane knobs and consist of an outpushing of the plasma membrane with a slight increase in its density. The out-pushing forms a spherical knob with an amorphous dense core and a total diameter of 500 to 600 Å. The knobs are found on axons and dendrites both in the spinal cord and peripherally.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221074
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  • 110
    Electronic Resource
    Electronic Resource
    Gap junctions in the heart of teleost fish (1974)
    Springer
    Cell & tissue research 154 (1974), S. 131-134 
    Details
    ISSN: 1432-0878
    Keywords: Heart musculature ; Teleost ; Gap junctions ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An examination of cell contacts was made in the atrial and ventricular muscle of Teleost fish. The intercalated discs consist of two types of junction resembling fascia and macula adherens. Small focal areas of gap junctions were shown to be numerous away from the regions of intercalated disc between the myocardial cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221077
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  • 111
    Electronic Resource
    Electronic Resource
    Ultrastructure of the neurosecretory system of the Colorado potato beetle, Leptinotarsa decemlineata (Say) (1974)
    Springer
    Cell & tissue research 154 (1974), S. 275-288 
    Details
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Insect ; Leptinotarsa decemlineata ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of seven types of neurosecretory cells (NSC) in the medial and lateral groups of the protocerebrum is described. The differences among cell types established earlier by light microscopy parallel differences in size and appearance of the neurosecretory particles observed in electron micrographs. No relationship was found between the affinity for Gomori's paraldehyde fuchsin stain and the nature of the particles. The secretions of the A-, A1-, and C-types of NSC of the medial group are characterized by electron-dense neurosecretory granules of 1250 Å dia., medium-dense granules of 2100 Å, and electron-lucent vesicles of 1700 Å, respectively. The L-type NSCof the lateral group contain smaller (1300 Å) or larger (1700 Å) neurosecretory granules. The medial B- and E-types of NSC and the lateral LB-type contain granulated vesicles (1200 Å) of the same appearance. These cell types differ in other respects and most likely have separate functions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223726
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  • 112
    Electronic Resource
    Electronic Resource
    Myofibroblasts in the rat testicular capsule (1974)
    Springer
    Cell & tissue research 154 (1974), S. 533-541 
    Details
    ISSN: 1432-0878
    Keywords: Myofibroblasts ; Testicular capsule (rat) ; Connective tissue ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A specialized type of fibroblast occurs in the testicular capsule of adult rats. The flattened cells are characterized by bundles of cytoplasmic filaments. Filament bundles run parallel to the cell surface and insert in plaques of granular, electron dense material which is attached to the inner surface of the plasma membrane (attachment zones). Cytoplasmic filaments measure 60–80 Å in diameter. Sporadically plaques of basal lamina-like material are found, especially in the region of attachment zones. These specialized fibroblasts are interpreted as myofibroblasts. It is supposed that contractility of the testicular capsule in rats is caused by myofibroblasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219672
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  • 113
    Electronic Resource
    Electronic Resource
    The ultrastructure of the sinus gland of Carcinus maenas (Crustacea: Decapoda) (1974)
    Springer
    Cell & tissue research 155 (1974), S. 117-125 
    Details
    ISSN: 1432-0878
    Keywords: Sinus gland ; Carcinus maenas (Crustacea, Decapoda) ; Nerve fibre cell types ; Neurosecretion ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The sinus gland of Carcinus maenas consists of the swollen axonal endings of the neurosecretory cells of the major ganglia and acts as a storage release centre for the membrane bound neurosecretory material. These neurosecretory granules fall into five different types based on size and electron density. Their contents are released by exocytosis of the primary granules or smaller units budded from the primary granules.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220288
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  • 114
    Electronic Resource
    Electronic Resource
    Fine structural and enzyme histochemical observations on the dorsal tail tubercle of Mertensiella caucasica (Urodela, Amphibia) (1974)
    Springer
    Cell & tissue research 156 (1974), S. 103-112 
    Details
    ISSN: 1432-0878
    Keywords: Dorsal tail tubercle ; Urodela ; Skin ; Hedonic glands ; Electron microscopy ; Enzyme histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Dorsal tubercle and skin of Mertensiella caucasica have been investigated with the electron microscope and enzyme histochemical methods. The epidermis of the tubercle consists of 8–9 cell layers, that of normal dorsal skin of 5–6. The tubercle is filled with large mucous glands which are surrounded by an almost complete layer of smooth muscle cells (myoepithelial cells). Their glandular cells undergo cyclical changes and are characterized by specific secretory granules, which differ from those of the relatively small mucous glands of the normal dorsal skin. In the connective tissue of the tubercle a relatively rich supply of nerve fibres has been found, which in part contain synaptic and dense core vesicles or accumulations of mitochondria. In the normal dorsal skin nerve fibres occur less frequently. The following enzymes have been demonstrated in the mucous glands of the tubercle: SDH, acid phosphatase, unspecific esterases, E 600 resistant esterase. The tubercle seems to stimulate the female cloaca chemically and mechanically.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220104
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